Both Mating Types of Phaeosphaeria (anamorph Stagonospora) nodorum are present in Western Australia

Phaeosphaeria (anamorph Stagonospora) nodorum is the most serious fungal pathogen of wheat in the West Australian (WA) wheat belt and is a diallelic heterothallic loculoascomycete. Its population genetics has received considerable attention. A recent study, which sampled isolates from diverse locati...

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Main Authors: Solomon, P., Parker, K., Loughman, R., Oliver, Richard
Format: Journal Article
Published: 2004
Online Access:http://hdl.handle.net/20.500.11937/27032
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author Solomon, P.
Parker, K.
Loughman, R.
Oliver, Richard
author_facet Solomon, P.
Parker, K.
Loughman, R.
Oliver, Richard
author_sort Solomon, P.
building Curtin Institutional Repository
collection Online Access
description Phaeosphaeria (anamorph Stagonospora) nodorum is the most serious fungal pathogen of wheat in the West Australian (WA) wheat belt and is a diallelic heterothallic loculoascomycete. Its population genetics has received considerable attention. A recent study, which sampled isolates from diverse locations worldwide, has indicated that the mating-type idiomorph MAT1-1 is considerably more frequent than MAT1-2 in many populations. To investigate this, we developed PCR primers that amplify each idiomorph. In a sample of 23 isolates cultured from ascospores collected in the field, nine amplified DNA with the MAT1-1 primers and 14 amplified DNA with the MAT1-2 primers. The virulence of a MAT1-2 isolate was comparable with MAT1-1 isolates. Although these sample sizes are small, we suggest that this result is consistent with the presence of equal numbers of both mating types in populations of ascospores in WA.
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institution Curtin University Malaysia
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spelling curtin-20.500.11937-270322017-09-13T16:08:35Z Both Mating Types of Phaeosphaeria (anamorph Stagonospora) nodorum are present in Western Australia Solomon, P. Parker, K. Loughman, R. Oliver, Richard Phaeosphaeria (anamorph Stagonospora) nodorum is the most serious fungal pathogen of wheat in the West Australian (WA) wheat belt and is a diallelic heterothallic loculoascomycete. Its population genetics has received considerable attention. A recent study, which sampled isolates from diverse locations worldwide, has indicated that the mating-type idiomorph MAT1-1 is considerably more frequent than MAT1-2 in many populations. To investigate this, we developed PCR primers that amplify each idiomorph. In a sample of 23 isolates cultured from ascospores collected in the field, nine amplified DNA with the MAT1-1 primers and 14 amplified DNA with the MAT1-2 primers. The virulence of a MAT1-2 isolate was comparable with MAT1-1 isolates. Although these sample sizes are small, we suggest that this result is consistent with the presence of equal numbers of both mating types in populations of ascospores in WA. 2004 Journal Article http://hdl.handle.net/20.500.11937/27032 10.1023/B:EJPP.0000041565.42836.c1 restricted
spellingShingle Solomon, P.
Parker, K.
Loughman, R.
Oliver, Richard
Both Mating Types of Phaeosphaeria (anamorph Stagonospora) nodorum are present in Western Australia
title Both Mating Types of Phaeosphaeria (anamorph Stagonospora) nodorum are present in Western Australia
title_full Both Mating Types of Phaeosphaeria (anamorph Stagonospora) nodorum are present in Western Australia
title_fullStr Both Mating Types of Phaeosphaeria (anamorph Stagonospora) nodorum are present in Western Australia
title_full_unstemmed Both Mating Types of Phaeosphaeria (anamorph Stagonospora) nodorum are present in Western Australia
title_short Both Mating Types of Phaeosphaeria (anamorph Stagonospora) nodorum are present in Western Australia
title_sort both mating types of phaeosphaeria (anamorph stagonospora) nodorum are present in western australia
url http://hdl.handle.net/20.500.11937/27032