Archaea Appear to Dominate the Microbiome of Inflatella pellicula Deep Sea Sponges
Microbes associated with marine sponges play significant roles in host physiology. Remarkable levels of microbial diversity have been observed in sponges worldwide through both culture-dependent and culture-independent studies. Most studies have focused on the structure of the bacterial communities...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
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Public Library of Science
2013
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| Online Access: | http://hdl.handle.net/20.500.11937/26815 |
| _version_ | 1848752093268017152 |
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| author | Jackson, Stephen Flemer, Burkhardt McCann, Angela Kennedy, Jonathan Morrissey, John O'Gara, Fergal Dobson, Alan |
| author_facet | Jackson, Stephen Flemer, Burkhardt McCann, Angela Kennedy, Jonathan Morrissey, John O'Gara, Fergal Dobson, Alan |
| author_sort | Jackson, Stephen |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Microbes associated with marine sponges play significant roles in host physiology. Remarkable levels of microbial diversity have been observed in sponges worldwide through both culture-dependent and culture-independent studies. Most studies have focused on the structure of the bacterial communities in sponges and have involved sponges sampled from shallow waters. Here, we used pyrosequencing of 16S rRNA genes to compare the bacterial and archaeal communities associated with two individuals of the marine sponge Inflatella pellicula from the deep-sea, sampled from a depth of 2,900 m, a depth which far exceeds any previous sequence-based report of sponge-associated microbial communities. Sponge-microbial communities were also compared to the microbial community in the surrounding seawater. Sponge-associated microbial communities were dominated by archaeal sequencing reads with a single archaeal OTU, comprising ~60% and ~72% of sequences, being observed from Inflatella pellicula. Archaeal sequencing reads were less abundant in seawater (~11% of sequences). Sponge-associated microbial communities were less diverse and less even than any other sponge-microbial community investigated to date with just 210 and 273 OTUs (97% sequence identity) identified in sponges, with 4 and 6 dominant OTUs comprising ~88% and ~89% of sequences, respectively. Members of the candidate phyla, SAR406, NC10 and ZB3 are reported here from sponges for the first time, increasing the number of bacterial phyla or candidate divisions associated with sponges to 43. A minor cohort from both sponge samples (~0.2% and ~0.3% of sequences) were not classified to phylum level. A single OTU, common to both sponge individuals, dominates these unclassified reads and shares sequence homology with a sponge associated clone which itself has no known close relative and may represent a novel taxon. |
| first_indexed | 2025-11-14T08:03:08Z |
| format | Journal Article |
| id | curtin-20.500.11937-26815 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:03:08Z |
| publishDate | 2013 |
| publisher | Public Library of Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-268152017-09-13T15:28:48Z Archaea Appear to Dominate the Microbiome of Inflatella pellicula Deep Sea Sponges Jackson, Stephen Flemer, Burkhardt McCann, Angela Kennedy, Jonathan Morrissey, John O'Gara, Fergal Dobson, Alan Microbes associated with marine sponges play significant roles in host physiology. Remarkable levels of microbial diversity have been observed in sponges worldwide through both culture-dependent and culture-independent studies. Most studies have focused on the structure of the bacterial communities in sponges and have involved sponges sampled from shallow waters. Here, we used pyrosequencing of 16S rRNA genes to compare the bacterial and archaeal communities associated with two individuals of the marine sponge Inflatella pellicula from the deep-sea, sampled from a depth of 2,900 m, a depth which far exceeds any previous sequence-based report of sponge-associated microbial communities. Sponge-microbial communities were also compared to the microbial community in the surrounding seawater. Sponge-associated microbial communities were dominated by archaeal sequencing reads with a single archaeal OTU, comprising ~60% and ~72% of sequences, being observed from Inflatella pellicula. Archaeal sequencing reads were less abundant in seawater (~11% of sequences). Sponge-associated microbial communities were less diverse and less even than any other sponge-microbial community investigated to date with just 210 and 273 OTUs (97% sequence identity) identified in sponges, with 4 and 6 dominant OTUs comprising ~88% and ~89% of sequences, respectively. Members of the candidate phyla, SAR406, NC10 and ZB3 are reported here from sponges for the first time, increasing the number of bacterial phyla or candidate divisions associated with sponges to 43. A minor cohort from both sponge samples (~0.2% and ~0.3% of sequences) were not classified to phylum level. A single OTU, common to both sponge individuals, dominates these unclassified reads and shares sequence homology with a sponge associated clone which itself has no known close relative and may represent a novel taxon. 2013 Journal Article http://hdl.handle.net/20.500.11937/26815 10.1371/journal.pone.0084438 Public Library of Science unknown |
| spellingShingle | Jackson, Stephen Flemer, Burkhardt McCann, Angela Kennedy, Jonathan Morrissey, John O'Gara, Fergal Dobson, Alan Archaea Appear to Dominate the Microbiome of Inflatella pellicula Deep Sea Sponges |
| title | Archaea Appear to Dominate the Microbiome of Inflatella pellicula Deep Sea Sponges |
| title_full | Archaea Appear to Dominate the Microbiome of Inflatella pellicula Deep Sea Sponges |
| title_fullStr | Archaea Appear to Dominate the Microbiome of Inflatella pellicula Deep Sea Sponges |
| title_full_unstemmed | Archaea Appear to Dominate the Microbiome of Inflatella pellicula Deep Sea Sponges |
| title_short | Archaea Appear to Dominate the Microbiome of Inflatella pellicula Deep Sea Sponges |
| title_sort | archaea appear to dominate the microbiome of inflatella pellicula deep sea sponges |
| url | http://hdl.handle.net/20.500.11937/26815 |