Voltammetric behaviour of biological macromolecules at arrays of aqueous|organogel micro-interfaces
The behaviour of two biological macromolecules, bovine pancreatic insulin and hen-egg-whitelysozyme (HEWL), at aqueous-organogel interfaces confined within an array of solid-state membrane micropores was investigated via cyclic voltammetry (CV). The behaviour observed is discussed in terms of possib...
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| Format: | Journal Article |
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Royal Society of Chemistry
2010
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| Online Access: | http://hdl.handle.net/20.500.11937/26812 |
| _version_ | 1848752092445933568 |
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| author | Scanlon, M. Strutwolf, J. Arrigan, Damien |
| author_facet | Scanlon, M. Strutwolf, J. Arrigan, Damien |
| author_sort | Scanlon, M. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The behaviour of two biological macromolecules, bovine pancreatic insulin and hen-egg-whitelysozyme (HEWL), at aqueous-organogel interfaces confined within an array of solid-state membrane micropores was investigated via cyclic voltammetry (CV). The behaviour observed is discussed in terms of possible charge transferring species and mass transport in the interfacial reaction. Comparison of CV results for HEWL, insulin, and the well-characterised model ion tetraethylammonium cation (TEA+) revealed that the biomacromolecules undergo an interfacial reaction comprising biomacromolecular adsorption and facilitated transfer of electrolyte anions from the organic phase to a protein layer on the aqueous side of the interface, whereas TEA+ undergoes a simple ion transfer process. Evidence for biomacromolecular adsorption on the aqueous side of the micro-interfaces is provided by comparison of the CVs for TEA+ ion transfer in the presence and absence of the biomacromolecules. Similar experiments in the presence of the low generation polypropylenimine tetraamine dendrimer, (DAB-AM-4), a smaller synthetic molecule, revealed it to be non-adsorbing. The behaviour of biological macromolecules at miniaturised aqueous-organogel interfaces involves adsorption on the aqueous side of the interface and transfer of organic phase electrolyte anions across the interface to associate with the adsorbed biomacro molecule. The data presented support the previously suggested mechanism forbiomacromolecular voltammetry at liquid-liquid interfaces, involving adsorption and facilitated ion-transfer of organic electrolyte anions. |
| first_indexed | 2025-11-14T08:03:07Z |
| format | Journal Article |
| id | curtin-20.500.11937-26812 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:03:07Z |
| publishDate | 2010 |
| publisher | Royal Society of Chemistry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-268122017-10-02T02:28:22Z Voltammetric behaviour of biological macromolecules at arrays of aqueous|organogel micro-interfaces Scanlon, M. Strutwolf, J. Arrigan, Damien The behaviour of two biological macromolecules, bovine pancreatic insulin and hen-egg-whitelysozyme (HEWL), at aqueous-organogel interfaces confined within an array of solid-state membrane micropores was investigated via cyclic voltammetry (CV). The behaviour observed is discussed in terms of possible charge transferring species and mass transport in the interfacial reaction. Comparison of CV results for HEWL, insulin, and the well-characterised model ion tetraethylammonium cation (TEA+) revealed that the biomacromolecules undergo an interfacial reaction comprising biomacromolecular adsorption and facilitated transfer of electrolyte anions from the organic phase to a protein layer on the aqueous side of the interface, whereas TEA+ undergoes a simple ion transfer process. Evidence for biomacromolecular adsorption on the aqueous side of the micro-interfaces is provided by comparison of the CVs for TEA+ ion transfer in the presence and absence of the biomacromolecules. Similar experiments in the presence of the low generation polypropylenimine tetraamine dendrimer, (DAB-AM-4), a smaller synthetic molecule, revealed it to be non-adsorbing. The behaviour of biological macromolecules at miniaturised aqueous-organogel interfaces involves adsorption on the aqueous side of the interface and transfer of organic phase electrolyte anions across the interface to associate with the adsorbed biomacro molecule. The data presented support the previously suggested mechanism forbiomacromolecular voltammetry at liquid-liquid interfaces, involving adsorption and facilitated ion-transfer of organic electrolyte anions. 2010 Journal Article http://hdl.handle.net/20.500.11937/26812 10.1039/c003323e Royal Society of Chemistry fulltext |
| spellingShingle | Scanlon, M. Strutwolf, J. Arrigan, Damien Voltammetric behaviour of biological macromolecules at arrays of aqueous|organogel micro-interfaces |
| title | Voltammetric behaviour of biological macromolecules at arrays of aqueous|organogel micro-interfaces |
| title_full | Voltammetric behaviour of biological macromolecules at arrays of aqueous|organogel micro-interfaces |
| title_fullStr | Voltammetric behaviour of biological macromolecules at arrays of aqueous|organogel micro-interfaces |
| title_full_unstemmed | Voltammetric behaviour of biological macromolecules at arrays of aqueous|organogel micro-interfaces |
| title_short | Voltammetric behaviour of biological macromolecules at arrays of aqueous|organogel micro-interfaces |
| title_sort | voltammetric behaviour of biological macromolecules at arrays of aqueous|organogel micro-interfaces |
| url | http://hdl.handle.net/20.500.11937/26812 |