Validation of a suite of biomarkers of fish health in the tropical bioindicator species, tambaqui (Colossoma macropomum)
Here we explore the dose-dependent response of the tropical fish tambaqui (Colossoma macropomum) to intraperitoneal injection of benzo[a]pyrene (BaP) at doses of 0 (carrier control), 1, 10, 100 and 1000 µmolar BaP Kg-1 Hepatic ethoxyresorufin-O-deethylase (EROD) activity showed a bell-shaped dose-de...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Published: |
Elsevier
2017
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| Online Access: | http://hdl.handle.net/20.500.11937/26722 |
| _version_ | 1848752067054665728 |
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| author | Sadauskas-Henrique, H. Duarte, R. Gagnon, Marthe Monique Almeida-Val, V. |
| author_facet | Sadauskas-Henrique, H. Duarte, R. Gagnon, Marthe Monique Almeida-Val, V. |
| author_sort | Sadauskas-Henrique, H. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Here we explore the dose-dependent response of the tropical fish tambaqui (Colossoma macropomum) to intraperitoneal injection of benzo[a]pyrene (BaP) at doses of 0 (carrier control), 1, 10, 100 and 1000 µmolar BaP Kg-1 Hepatic ethoxyresorufin-O-deethylase (EROD) activity showed a bell-shaped dose-dependent response curve, where the highest injected BaP dose caused enzyme inactivation. Activities of hepatic catalase (CAT) and superoxide dismutase (SOD) increased at the highest dose relative to the carrier control group. Lipid peroxidation (LPO), serum-sorbitol dehydrogenase (s-SDH) and DNA damage in blood cells were higher for all BaP doses when compared to the carrier control group. At high dosage, the production of BaP metabolites was paralleled by induced activity of the antioxidant enzyme SOD, and high levels of DNA damage in blood cells. In a similar way, high LPO was concomitant to elevated s-SDH in the bloodstream, suggesting that lipid peroxidation caused the loss of membrane integrity and leakage of s-SDH from hepatocytes into the bloodstream. These biomarkers were also positively co-correlated. The results demonstrate the potential use of a suite of biomarkers for tambaqui living in contaminated tropical aquatic environments. In particular, we recommend the analysis of DNA damage in blood cells, as this was highly correlated with all other biomarkers. © 2016 Elsevier Ltd |
| first_indexed | 2025-11-14T08:02:43Z |
| format | Journal Article |
| id | curtin-20.500.11937-26722 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:02:43Z |
| publishDate | 2017 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-267222018-10-26T03:26:55Z Validation of a suite of biomarkers of fish health in the tropical bioindicator species, tambaqui (Colossoma macropomum) Sadauskas-Henrique, H. Duarte, R. Gagnon, Marthe Monique Almeida-Val, V. Here we explore the dose-dependent response of the tropical fish tambaqui (Colossoma macropomum) to intraperitoneal injection of benzo[a]pyrene (BaP) at doses of 0 (carrier control), 1, 10, 100 and 1000 µmolar BaP Kg-1 Hepatic ethoxyresorufin-O-deethylase (EROD) activity showed a bell-shaped dose-dependent response curve, where the highest injected BaP dose caused enzyme inactivation. Activities of hepatic catalase (CAT) and superoxide dismutase (SOD) increased at the highest dose relative to the carrier control group. Lipid peroxidation (LPO), serum-sorbitol dehydrogenase (s-SDH) and DNA damage in blood cells were higher for all BaP doses when compared to the carrier control group. At high dosage, the production of BaP metabolites was paralleled by induced activity of the antioxidant enzyme SOD, and high levels of DNA damage in blood cells. In a similar way, high LPO was concomitant to elevated s-SDH in the bloodstream, suggesting that lipid peroxidation caused the loss of membrane integrity and leakage of s-SDH from hepatocytes into the bloodstream. These biomarkers were also positively co-correlated. The results demonstrate the potential use of a suite of biomarkers for tambaqui living in contaminated tropical aquatic environments. In particular, we recommend the analysis of DNA damage in blood cells, as this was highly correlated with all other biomarkers. © 2016 Elsevier Ltd 2017 Journal Article http://hdl.handle.net/20.500.11937/26722 10.1016/j.ecolind.2016.10.010 Elsevier fulltext |
| spellingShingle | Sadauskas-Henrique, H. Duarte, R. Gagnon, Marthe Monique Almeida-Val, V. Validation of a suite of biomarkers of fish health in the tropical bioindicator species, tambaqui (Colossoma macropomum) |
| title | Validation of a suite of biomarkers of fish health in the tropical bioindicator species, tambaqui (Colossoma macropomum) |
| title_full | Validation of a suite of biomarkers of fish health in the tropical bioindicator species, tambaqui (Colossoma macropomum) |
| title_fullStr | Validation of a suite of biomarkers of fish health in the tropical bioindicator species, tambaqui (Colossoma macropomum) |
| title_full_unstemmed | Validation of a suite of biomarkers of fish health in the tropical bioindicator species, tambaqui (Colossoma macropomum) |
| title_short | Validation of a suite of biomarkers of fish health in the tropical bioindicator species, tambaqui (Colossoma macropomum) |
| title_sort | validation of a suite of biomarkers of fish health in the tropical bioindicator species, tambaqui (colossoma macropomum) |
| url | http://hdl.handle.net/20.500.11937/26722 |