Overexpression of the malate-aspartate NADH shuttle member Aralar1 in the clonal ß-cell line BRIN-BD11 enhances amino-acid-stimulated insulin secretion and cell metabolism
In the present study, we have investigated the effects of the transduction with recombinant adenovirus AdCA-Aralar1 (aspartate-glutamate carrier 1) on the metabolism, function and secretory properties of the glucose- and amino-acid-responsive clonal insulin-secreting cell line BRIN-BD11. Aralar1 ove...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Published: |
Portland Press
2009
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| Online Access: | http://hdl.handle.net/20.500.11937/26084 |
| _version_ | 1848751884793282560 |
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| author | Bender, K. Maechler, P. McClenaghan, N. Flatt, P. Newsholme, Philip |
| author_facet | Bender, K. Maechler, P. McClenaghan, N. Flatt, P. Newsholme, Philip |
| author_sort | Bender, K. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | In the present study, we have investigated the effects of the transduction with recombinant adenovirus AdCA-Aralar1 (aspartate-glutamate carrier 1) on the metabolism, function and secretory properties of the glucose- and amino-acid-responsive clonal insulin-secreting cell line BRIN-BD11. Aralar1 overexpression increased long-term (24 h) and acute (20 min) glucose- and amino-acid-stimulated insulin secretion, cellular glucose metabolism, L-alanine and L-glutamine consumption, cellular ATP and glutamate concentrations, and stimulated glutamate release. However, cellular triacylglycerol and glycogen contents were decreased as was lactate production. These findings indicate that increased malate-aspartate shuttle activity positively shifted ß-cell metabolism, thereby increasing glycolysis capacity, stimulus-secretion coupling and, ultimately, enhancing insulin secretion. We conclude that Aralar1 is a key metabolic control site in insulin-secreting cells. |
| first_indexed | 2025-11-14T07:59:49Z |
| format | Journal Article |
| id | curtin-20.500.11937-26084 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:59:49Z |
| publishDate | 2009 |
| publisher | Portland Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-260842017-09-13T15:24:20Z Overexpression of the malate-aspartate NADH shuttle member Aralar1 in the clonal ß-cell line BRIN-BD11 enhances amino-acid-stimulated insulin secretion and cell metabolism Bender, K. Maechler, P. McClenaghan, N. Flatt, P. Newsholme, Philip In the present study, we have investigated the effects of the transduction with recombinant adenovirus AdCA-Aralar1 (aspartate-glutamate carrier 1) on the metabolism, function and secretory properties of the glucose- and amino-acid-responsive clonal insulin-secreting cell line BRIN-BD11. Aralar1 overexpression increased long-term (24 h) and acute (20 min) glucose- and amino-acid-stimulated insulin secretion, cellular glucose metabolism, L-alanine and L-glutamine consumption, cellular ATP and glutamate concentrations, and stimulated glutamate release. However, cellular triacylglycerol and glycogen contents were decreased as was lactate production. These findings indicate that increased malate-aspartate shuttle activity positively shifted ß-cell metabolism, thereby increasing glycolysis capacity, stimulus-secretion coupling and, ultimately, enhancing insulin secretion. We conclude that Aralar1 is a key metabolic control site in insulin-secreting cells. 2009 Journal Article http://hdl.handle.net/20.500.11937/26084 10.1042/CS20090126 Portland Press unknown |
| spellingShingle | Bender, K. Maechler, P. McClenaghan, N. Flatt, P. Newsholme, Philip Overexpression of the malate-aspartate NADH shuttle member Aralar1 in the clonal ß-cell line BRIN-BD11 enhances amino-acid-stimulated insulin secretion and cell metabolism |
| title | Overexpression of the malate-aspartate NADH shuttle member Aralar1 in the clonal ß-cell line BRIN-BD11 enhances amino-acid-stimulated insulin secretion and cell metabolism |
| title_full | Overexpression of the malate-aspartate NADH shuttle member Aralar1 in the clonal ß-cell line BRIN-BD11 enhances amino-acid-stimulated insulin secretion and cell metabolism |
| title_fullStr | Overexpression of the malate-aspartate NADH shuttle member Aralar1 in the clonal ß-cell line BRIN-BD11 enhances amino-acid-stimulated insulin secretion and cell metabolism |
| title_full_unstemmed | Overexpression of the malate-aspartate NADH shuttle member Aralar1 in the clonal ß-cell line BRIN-BD11 enhances amino-acid-stimulated insulin secretion and cell metabolism |
| title_short | Overexpression of the malate-aspartate NADH shuttle member Aralar1 in the clonal ß-cell line BRIN-BD11 enhances amino-acid-stimulated insulin secretion and cell metabolism |
| title_sort | overexpression of the malate-aspartate nadh shuttle member aralar1 in the clonal ß-cell line brin-bd11 enhances amino-acid-stimulated insulin secretion and cell metabolism |
| url | http://hdl.handle.net/20.500.11937/26084 |