Boyden chamber

The Boyden chamber, initially designed to study leukocyte chemotaxis, has become one of the most used tools to assess cell motility and invasion. The classical Boyden chamber consists of two compartments separated by a membrane representing a physical barrier that cells can overcome only by active m...

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Main Authors: Falasca, Marco, Raimondi, C., Maffucci, T.
Format: Book Chapter
Published: 2011
Online Access:http://hdl.handle.net/20.500.11937/26064
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author Falasca, Marco
Raimondi, C.
Maffucci, T.
author_facet Falasca, Marco
Raimondi, C.
Maffucci, T.
author_sort Falasca, Marco
building Curtin Institutional Repository
collection Online Access
description The Boyden chamber, initially designed to study leukocyte chemotaxis, has become one of the most used tools to assess cell motility and invasion. The classical Boyden chamber consists of two compartments separated by a membrane representing a physical barrier that cells can overcome only by active migration. Since its initial introduction, a number of different Boyden chamber devices have been developed. The Boyden chamber can be adapted to study tumour cells' invasive properties by coating the membrane with different extracellular matrix proteins. The method described in this chapter is intended specifically for measuring the migration or invasion of human endothelial and cancer cells.
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format Book Chapter
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institution Curtin University Malaysia
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publishDate 2011
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spelling curtin-20.500.11937-260642017-09-13T15:23:29Z Boyden chamber Falasca, Marco Raimondi, C. Maffucci, T. The Boyden chamber, initially designed to study leukocyte chemotaxis, has become one of the most used tools to assess cell motility and invasion. The classical Boyden chamber consists of two compartments separated by a membrane representing a physical barrier that cells can overcome only by active migration. Since its initial introduction, a number of different Boyden chamber devices have been developed. The Boyden chamber can be adapted to study tumour cells' invasive properties by coating the membrane with different extracellular matrix proteins. The method described in this chapter is intended specifically for measuring the migration or invasion of human endothelial and cancer cells. 2011 Book Chapter http://hdl.handle.net/20.500.11937/26064 10.1007/978-1-61779-207-6_7 restricted
spellingShingle Falasca, Marco
Raimondi, C.
Maffucci, T.
Boyden chamber
title Boyden chamber
title_full Boyden chamber
title_fullStr Boyden chamber
title_full_unstemmed Boyden chamber
title_short Boyden chamber
title_sort boyden chamber
url http://hdl.handle.net/20.500.11937/26064