Robust interferon-a and IL-12 responses by dendritic cells are related to efficient CD4+ T-cell recovery in HIV patients on ART

Amongst HIV patients with successful virological responses to antiretroviral therapy (ART), poor CD4+ T-cell recovery is associated with low nadir CD4+ T-cell counts and persistent immune activation. These factors might be influenced by dendritic cell (DC) function. Interferon-a-producing plasmacyto...

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Main Authors: Tan, D., Yong, Y., Lim, A., Tan, H., Kamarulzaman, A., French, M., Price, Patricia
Format: Journal Article
Published: 2011
Online Access:http://hdl.handle.net/20.500.11937/25996
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author Tan, D.
Yong, Y.
Lim, A.
Tan, H.
Kamarulzaman, A.
French, M.
Price, Patricia
author_facet Tan, D.
Yong, Y.
Lim, A.
Tan, H.
Kamarulzaman, A.
French, M.
Price, Patricia
author_sort Tan, D.
building Curtin Institutional Repository
collection Online Access
description Amongst HIV patients with successful virological responses to antiretroviral therapy (ART), poor CD4+ T-cell recovery is associated with low nadir CD4+ T-cell counts and persistent immune activation. These factors might be influenced by dendritic cell (DC) function. Interferon-a-producing plasmacytoid DC and IL-12-producing myeloid DC were quantified by flow cytometry after stimulation with agonists to TLR7/8 (CL075) or TLR9 (CpG-ODN). These were compared between patients who achieved CD4+ T-cell counts above or below 200 cells/µL after 6months on ART (High vs. Low groups). High Group patients had more DC producing interferon-a or IL-12 at Weeks 6 and 12 on ART than Low Group patients. The frequencies of cytokine-producing DC at Week 12 were directly correlated with CD4+ T-cell counts at baseline and at Week 12. Patients with good recovery of CD4+ T-cells had robust TLR-mediated interferon-a responses by plasmacytoid DC and IL-12 responses by myeloid DC during early ART (1-3months). © 2011 Elsevier Inc.
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spelling curtin-20.500.11937-259962017-09-13T15:23:54Z Robust interferon-a and IL-12 responses by dendritic cells are related to efficient CD4+ T-cell recovery in HIV patients on ART Tan, D. Yong, Y. Lim, A. Tan, H. Kamarulzaman, A. French, M. Price, Patricia Amongst HIV patients with successful virological responses to antiretroviral therapy (ART), poor CD4+ T-cell recovery is associated with low nadir CD4+ T-cell counts and persistent immune activation. These factors might be influenced by dendritic cell (DC) function. Interferon-a-producing plasmacytoid DC and IL-12-producing myeloid DC were quantified by flow cytometry after stimulation with agonists to TLR7/8 (CL075) or TLR9 (CpG-ODN). These were compared between patients who achieved CD4+ T-cell counts above or below 200 cells/µL after 6months on ART (High vs. Low groups). High Group patients had more DC producing interferon-a or IL-12 at Weeks 6 and 12 on ART than Low Group patients. The frequencies of cytokine-producing DC at Week 12 were directly correlated with CD4+ T-cell counts at baseline and at Week 12. Patients with good recovery of CD4+ T-cells had robust TLR-mediated interferon-a responses by plasmacytoid DC and IL-12 responses by myeloid DC during early ART (1-3months). © 2011 Elsevier Inc. 2011 Journal Article http://hdl.handle.net/20.500.11937/25996 10.1016/j.clim.2011.02.015 restricted
spellingShingle Tan, D.
Yong, Y.
Lim, A.
Tan, H.
Kamarulzaman, A.
French, M.
Price, Patricia
Robust interferon-a and IL-12 responses by dendritic cells are related to efficient CD4+ T-cell recovery in HIV patients on ART
title Robust interferon-a and IL-12 responses by dendritic cells are related to efficient CD4+ T-cell recovery in HIV patients on ART
title_full Robust interferon-a and IL-12 responses by dendritic cells are related to efficient CD4+ T-cell recovery in HIV patients on ART
title_fullStr Robust interferon-a and IL-12 responses by dendritic cells are related to efficient CD4+ T-cell recovery in HIV patients on ART
title_full_unstemmed Robust interferon-a and IL-12 responses by dendritic cells are related to efficient CD4+ T-cell recovery in HIV patients on ART
title_short Robust interferon-a and IL-12 responses by dendritic cells are related to efficient CD4+ T-cell recovery in HIV patients on ART
title_sort robust interferon-a and il-12 responses by dendritic cells are related to efficient cd4+ t-cell recovery in hiv patients on art
url http://hdl.handle.net/20.500.11937/25996