High diversity of picornaviruses in rats from different continents revealed by deep sequencing
Outbreaks of zoonotic diseases in humans and livestock are not uncommon, and an important component in containment of such emerging viral diseases is rapid and reliable diagnostics. Such methods are often PCR-based and hence require the availability of sequence data from the pathogen. Rattus norvegi...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
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Nature Publishing Group
2016
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| Online Access: | http://hdl.handle.net/20.500.11937/25946 |
| _version_ | 1848751847124238336 |
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| author | Hansen, T. Mollerup, S. Nguyen, N. White, Nicole Coghlan, Megan Alquezar-Planas, D. Joshi, T. Jensen, R. Fridholm, H. Kjartansdóttir, K. Mourier, T. Warnow, T. Belsham, G. Bunce, Michael Willerslev, E. Nielsen, L. Vinner, L. Hansen, A. |
| author_facet | Hansen, T. Mollerup, S. Nguyen, N. White, Nicole Coghlan, Megan Alquezar-Planas, D. Joshi, T. Jensen, R. Fridholm, H. Kjartansdóttir, K. Mourier, T. Warnow, T. Belsham, G. Bunce, Michael Willerslev, E. Nielsen, L. Vinner, L. Hansen, A. |
| author_sort | Hansen, T. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Outbreaks of zoonotic diseases in humans and livestock are not uncommon, and an important component in containment of such emerging viral diseases is rapid and reliable diagnostics. Such methods are often PCR-based and hence require the availability of sequence data from the pathogen. Rattus norvegicus (R. norvegicus) is a known reservoir for important zoonotic pathogens. Transmission may be direct via contact with the animal, for example, through exposure to its faecal matter, or indirectly mediated by arthropod vectors. Here we investigated the viral content in rat faecal matter (n=29) collected from two continents by analyzing 2.2 billion next-generation sequencing reads derived from both DNA and RNA. Among other virus families, we found sequences from members of the Picornaviridae to be abundant in the microbiome of all the samples. Here we describe the diversity of the picornavirus-like contigs including near-full-length genomes closely related to the Boone cardiovirus and Theiler's encephalomyelitis virus. From this study, we conclude that picornaviruses within R. norvegicus are more diverse than previously recognized. The virome of R. norvegicus should be investigated further to assess the full potential for zoonotic virus transmission. |
| first_indexed | 2025-11-14T07:59:13Z |
| format | Journal Article |
| id | curtin-20.500.11937-25946 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:59:13Z |
| publishDate | 2016 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-259462017-09-13T15:23:04Z High diversity of picornaviruses in rats from different continents revealed by deep sequencing Hansen, T. Mollerup, S. Nguyen, N. White, Nicole Coghlan, Megan Alquezar-Planas, D. Joshi, T. Jensen, R. Fridholm, H. Kjartansdóttir, K. Mourier, T. Warnow, T. Belsham, G. Bunce, Michael Willerslev, E. Nielsen, L. Vinner, L. Hansen, A. Outbreaks of zoonotic diseases in humans and livestock are not uncommon, and an important component in containment of such emerging viral diseases is rapid and reliable diagnostics. Such methods are often PCR-based and hence require the availability of sequence data from the pathogen. Rattus norvegicus (R. norvegicus) is a known reservoir for important zoonotic pathogens. Transmission may be direct via contact with the animal, for example, through exposure to its faecal matter, or indirectly mediated by arthropod vectors. Here we investigated the viral content in rat faecal matter (n=29) collected from two continents by analyzing 2.2 billion next-generation sequencing reads derived from both DNA and RNA. Among other virus families, we found sequences from members of the Picornaviridae to be abundant in the microbiome of all the samples. Here we describe the diversity of the picornavirus-like contigs including near-full-length genomes closely related to the Boone cardiovirus and Theiler's encephalomyelitis virus. From this study, we conclude that picornaviruses within R. norvegicus are more diverse than previously recognized. The virome of R. norvegicus should be investigated further to assess the full potential for zoonotic virus transmission. 2016 Journal Article http://hdl.handle.net/20.500.11937/25946 10.1038/emi.2016.90 Nature Publishing Group fulltext |
| spellingShingle | Hansen, T. Mollerup, S. Nguyen, N. White, Nicole Coghlan, Megan Alquezar-Planas, D. Joshi, T. Jensen, R. Fridholm, H. Kjartansdóttir, K. Mourier, T. Warnow, T. Belsham, G. Bunce, Michael Willerslev, E. Nielsen, L. Vinner, L. Hansen, A. High diversity of picornaviruses in rats from different continents revealed by deep sequencing |
| title | High diversity of picornaviruses in rats from different continents revealed by deep sequencing |
| title_full | High diversity of picornaviruses in rats from different continents revealed by deep sequencing |
| title_fullStr | High diversity of picornaviruses in rats from different continents revealed by deep sequencing |
| title_full_unstemmed | High diversity of picornaviruses in rats from different continents revealed by deep sequencing |
| title_short | High diversity of picornaviruses in rats from different continents revealed by deep sequencing |
| title_sort | high diversity of picornaviruses in rats from different continents revealed by deep sequencing |
| url | http://hdl.handle.net/20.500.11937/25946 |