Molecular characterization of endocarditis-associated Staphylococcus aureus
Infective endocarditis (IE) is a life-threatening infection of the heart endothelium and valves. Staphylococcus aureus is a predominant cause of severe IE and is frequently associated with infections in health care settings and device-related infections. Multilocus sequence typing (MLST), spa typing...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
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American Society for Microbiology
2013
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| Online Access: | http://hdl.handle.net/20.500.11937/25883 |
| _version_ | 1848751830011478016 |
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| author | Nethercott, C. Mabbett, A. Totsika, M. Peters, P. Ortiz, J. Nimmo, G. Coombs, Geoffrey Walker, M. Schembri, M. |
| author_facet | Nethercott, C. Mabbett, A. Totsika, M. Peters, P. Ortiz, J. Nimmo, G. Coombs, Geoffrey Walker, M. Schembri, M. |
| author_sort | Nethercott, C. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Infective endocarditis (IE) is a life-threatening infection of the heart endothelium and valves. Staphylococcus aureus is a predominant cause of severe IE and is frequently associated with infections in health care settings and device-related infections. Multilocus sequence typing (MLST), spa typing, and virulence gene microarrays are frequently used to classify S. aureus clinical isolates. This study examined the utility of these typing tools to investigate S. aureus epidemiology associated with IE. Ninety-seven S. aureus isolates were collected from patients diagnosed with (i) IE, (ii) bloodstream infection related to medical devices, (iii) bloodstream infection not related to medical devices, and (iv) skin or soft-tissue infections. The MLST clonal complex (CC) for each isolate was determined and compared to the CCs of members of the S. aureus population by eBURST analysis. The spa type of all isolates was also determined. A null model was used to determine correlations of IE with CC and spa type. DNA microarray analysis was performed, and a permutational analysis of multivariate variance (PERMANOVA) and principal coordinates analysis were conducted to identify genotypic differences between IE and non-IE strains. CC12, CC20, and spa type t160 were significantly associated with IE S. aureus. A subset of virulence-associated genes and alleles, including genes encoding staphylococcal superantigen-like proteins, fibrinogen-binding protein, and a leukocidin subunit, also significantly correlated with IE isolates. MLST, spa typing, and microarray analysis are promising tools for monitoring S. aureus epidemiology associated with IE. Further research to determine a role for the S. aureus IE-associated virulence genes identified in this study is warranted. |
| first_indexed | 2025-11-14T07:58:57Z |
| format | Journal Article |
| id | curtin-20.500.11937-25883 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:58:57Z |
| publishDate | 2013 |
| publisher | American Society for Microbiology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-258832023-02-22T06:24:20Z Molecular characterization of endocarditis-associated Staphylococcus aureus Nethercott, C. Mabbett, A. Totsika, M. Peters, P. Ortiz, J. Nimmo, G. Coombs, Geoffrey Walker, M. Schembri, M. Infective endocarditis (IE) is a life-threatening infection of the heart endothelium and valves. Staphylococcus aureus is a predominant cause of severe IE and is frequently associated with infections in health care settings and device-related infections. Multilocus sequence typing (MLST), spa typing, and virulence gene microarrays are frequently used to classify S. aureus clinical isolates. This study examined the utility of these typing tools to investigate S. aureus epidemiology associated with IE. Ninety-seven S. aureus isolates were collected from patients diagnosed with (i) IE, (ii) bloodstream infection related to medical devices, (iii) bloodstream infection not related to medical devices, and (iv) skin or soft-tissue infections. The MLST clonal complex (CC) for each isolate was determined and compared to the CCs of members of the S. aureus population by eBURST analysis. The spa type of all isolates was also determined. A null model was used to determine correlations of IE with CC and spa type. DNA microarray analysis was performed, and a permutational analysis of multivariate variance (PERMANOVA) and principal coordinates analysis were conducted to identify genotypic differences between IE and non-IE strains. CC12, CC20, and spa type t160 were significantly associated with IE S. aureus. A subset of virulence-associated genes and alleles, including genes encoding staphylococcal superantigen-like proteins, fibrinogen-binding protein, and a leukocidin subunit, also significantly correlated with IE isolates. MLST, spa typing, and microarray analysis are promising tools for monitoring S. aureus epidemiology associated with IE. Further research to determine a role for the S. aureus IE-associated virulence genes identified in this study is warranted. 2013 Journal Article http://hdl.handle.net/20.500.11937/25883 10.1128/JCM.00651-13 American Society for Microbiology unknown |
| spellingShingle | Nethercott, C. Mabbett, A. Totsika, M. Peters, P. Ortiz, J. Nimmo, G. Coombs, Geoffrey Walker, M. Schembri, M. Molecular characterization of endocarditis-associated Staphylococcus aureus |
| title | Molecular characterization of endocarditis-associated Staphylococcus aureus |
| title_full | Molecular characterization of endocarditis-associated Staphylococcus aureus |
| title_fullStr | Molecular characterization of endocarditis-associated Staphylococcus aureus |
| title_full_unstemmed | Molecular characterization of endocarditis-associated Staphylococcus aureus |
| title_short | Molecular characterization of endocarditis-associated Staphylococcus aureus |
| title_sort | molecular characterization of endocarditis-associated staphylococcus aureus |
| url | http://hdl.handle.net/20.500.11937/25883 |