The role of conserved lymphokine element 0 in induction and inhibition of interleukin-5
The role of eosinophilia in allergic disorders indicates hIL-5 as a target of therapy. The conservation of hIL-5 proximal elements suggests they are important in controlling expression. Corticosteroids are important in the treatment of allergy, and are powerful inhibitors of IL-5 expression. Antisen...
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| Format: | Thesis |
| Language: | English |
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Curtin University
2004
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| Online Access: | http://hdl.handle.net/20.500.11937/2557 |
| _version_ | 1848743986793021440 |
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| author | Arthaningtyas, Estri |
| author_facet | Arthaningtyas, Estri |
| author_sort | Arthaningtyas, Estri |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The role of eosinophilia in allergic disorders indicates hIL-5 as a target of therapy. The conservation of hIL-5 proximal elements suggests they are important in controlling expression. Corticosteroids are important in the treatment of allergy, and are powerful inhibitors of IL-5 expression. Antisense oligonucleotides are new compounds that can specifically inhibit IL-5 production. This study aimed at understanding the role of conserved lymphokine element 0 (CLEO) in induction and inhibition of IL-5.The conserved proximal CLEO/TATA elements driving a luciferase reporter gene gave higher expression than a 500bp promoter in PER1 17 T-cell line. Two and three copies of IL-5 CLEO upstream of the silent IL-4 minimal promoter gave 150-200 fold increases in expression in forward orientation, but little activity in reverse orientation. Consequently, while CLEO is a powerful activator, it is not a classical enhancer. Antisense technology has also shown the dependence of IL-5 gene transcription on the de novo synthesis of the transcription factor Fra2.Inhibition of IL-5 reporter constructs by dexamethasone when induced by PMNcAMP, but not PMNCaI, provided a tool for understanding the mechanism. Deletion analysis identified CLEO as the key element of dexamethasone inhibition. Non-inhibition of IL-5 reporter constructs by dexamethasone in a Jurkat cell line, however, showed a possible intermediary factor involved in the inhibition mechanism. |
| first_indexed | 2025-11-14T05:54:17Z |
| format | Thesis |
| id | curtin-20.500.11937-2557 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T05:54:17Z |
| publishDate | 2004 |
| publisher | Curtin University |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-25572017-02-20T06:39:00Z The role of conserved lymphokine element 0 in induction and inhibition of interleukin-5 Arthaningtyas, Estri DNA manipulations regulatory elements eosinophilia interleukin-5 The role of eosinophilia in allergic disorders indicates hIL-5 as a target of therapy. The conservation of hIL-5 proximal elements suggests they are important in controlling expression. Corticosteroids are important in the treatment of allergy, and are powerful inhibitors of IL-5 expression. Antisense oligonucleotides are new compounds that can specifically inhibit IL-5 production. This study aimed at understanding the role of conserved lymphokine element 0 (CLEO) in induction and inhibition of IL-5.The conserved proximal CLEO/TATA elements driving a luciferase reporter gene gave higher expression than a 500bp promoter in PER1 17 T-cell line. Two and three copies of IL-5 CLEO upstream of the silent IL-4 minimal promoter gave 150-200 fold increases in expression in forward orientation, but little activity in reverse orientation. Consequently, while CLEO is a powerful activator, it is not a classical enhancer. Antisense technology has also shown the dependence of IL-5 gene transcription on the de novo synthesis of the transcription factor Fra2.Inhibition of IL-5 reporter constructs by dexamethasone when induced by PMNcAMP, but not PMNCaI, provided a tool for understanding the mechanism. Deletion analysis identified CLEO as the key element of dexamethasone inhibition. Non-inhibition of IL-5 reporter constructs by dexamethasone in a Jurkat cell line, however, showed a possible intermediary factor involved in the inhibition mechanism. 2004 Thesis http://hdl.handle.net/20.500.11937/2557 en Curtin University fulltext |
| spellingShingle | DNA manipulations regulatory elements eosinophilia interleukin-5 Arthaningtyas, Estri The role of conserved lymphokine element 0 in induction and inhibition of interleukin-5 |
| title | The role of conserved lymphokine element 0 in induction and inhibition of interleukin-5 |
| title_full | The role of conserved lymphokine element 0 in induction and inhibition of interleukin-5 |
| title_fullStr | The role of conserved lymphokine element 0 in induction and inhibition of interleukin-5 |
| title_full_unstemmed | The role of conserved lymphokine element 0 in induction and inhibition of interleukin-5 |
| title_short | The role of conserved lymphokine element 0 in induction and inhibition of interleukin-5 |
| title_sort | role of conserved lymphokine element 0 in induction and inhibition of interleukin-5 |
| topic | DNA manipulations regulatory elements eosinophilia interleukin-5 |
| url | http://hdl.handle.net/20.500.11937/2557 |