Electrochemical Characterization of Regularly-aligned Nanopore Array Membranes Filled with Electrolyte Solutions and their use for Detection of Nucleic Acid Hybridization

We report on the electrochemical characterization of regularlyaligned cylindrical nanopore arrays supported in silicon nitride membranes and preliminary results for the detection of nucleic acid hybridization on the nanopore walls. A range of nanopore arrays with diameters between 40 and 150 nm were...

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Main Authors: Ellis, J., Herzog, G., Glynn, B., Arrigan, Damien
Format: Journal Article
Published: The Electrochemical Society 2011
Online Access:http://hdl.handle.net/20.500.11937/25329
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author Ellis, J.
Herzog, G.
Glynn, B.
Arrigan, Damien
author_facet Ellis, J.
Herzog, G.
Glynn, B.
Arrigan, Damien
author_sort Ellis, J.
building Curtin Institutional Repository
collection Online Access
description We report on the electrochemical characterization of regularlyaligned cylindrical nanopore arrays supported in silicon nitride membranes and preliminary results for the detection of nucleic acid hybridization on the nanopore walls. A range of nanopore arrays with diameters between 40 and 150 nm were examined. We tested the effect of pore diameter, number of pores, electrolyte concentration and surface chemistry on the conductance of the nanopore membranes. The pores were functionalized with singlestranded DNA and conductance measurements were performed before and after hybridization. In many cases, changes in current rectification were observed following hybridization, which is discussed as a strategy for nucleic acid hybridization and interactions.
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publishDate 2011
publisher The Electrochemical Society
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spelling curtin-20.500.11937-253292017-09-13T16:07:21Z Electrochemical Characterization of Regularly-aligned Nanopore Array Membranes Filled with Electrolyte Solutions and their use for Detection of Nucleic Acid Hybridization Ellis, J. Herzog, G. Glynn, B. Arrigan, Damien We report on the electrochemical characterization of regularlyaligned cylindrical nanopore arrays supported in silicon nitride membranes and preliminary results for the detection of nucleic acid hybridization on the nanopore walls. A range of nanopore arrays with diameters between 40 and 150 nm were examined. We tested the effect of pore diameter, number of pores, electrolyte concentration and surface chemistry on the conductance of the nanopore membranes. The pores were functionalized with singlestranded DNA and conductance measurements were performed before and after hybridization. In many cases, changes in current rectification were observed following hybridization, which is discussed as a strategy for nucleic acid hybridization and interactions. 2011 Journal Article http://hdl.handle.net/20.500.11937/25329 10.1149/1.3571974 The Electrochemical Society fulltext
spellingShingle Ellis, J.
Herzog, G.
Glynn, B.
Arrigan, Damien
Electrochemical Characterization of Regularly-aligned Nanopore Array Membranes Filled with Electrolyte Solutions and their use for Detection of Nucleic Acid Hybridization
title Electrochemical Characterization of Regularly-aligned Nanopore Array Membranes Filled with Electrolyte Solutions and their use for Detection of Nucleic Acid Hybridization
title_full Electrochemical Characterization of Regularly-aligned Nanopore Array Membranes Filled with Electrolyte Solutions and their use for Detection of Nucleic Acid Hybridization
title_fullStr Electrochemical Characterization of Regularly-aligned Nanopore Array Membranes Filled with Electrolyte Solutions and their use for Detection of Nucleic Acid Hybridization
title_full_unstemmed Electrochemical Characterization of Regularly-aligned Nanopore Array Membranes Filled with Electrolyte Solutions and their use for Detection of Nucleic Acid Hybridization
title_short Electrochemical Characterization of Regularly-aligned Nanopore Array Membranes Filled with Electrolyte Solutions and their use for Detection of Nucleic Acid Hybridization
title_sort electrochemical characterization of regularly-aligned nanopore array membranes filled with electrolyte solutions and their use for detection of nucleic acid hybridization
url http://hdl.handle.net/20.500.11937/25329