High-Contrast Visualization of Upconversion Luminescence in Mice Using Time-Gating Approach
© 2016 American Chemical Society. Optical imaging through the near-infrared (NIR) window provides deep penetration of light up to several centimeters into biological tissues. Capable of emitting 800 nm luminescence under 980 nm illumination, the recently developed upconversion nanoparticles (UCNPs)...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
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American Chemical Society
2016
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| Online Access: | http://hdl.handle.net/20.500.11937/25271 |
| _version_ | 1848751662048477184 |
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| author | Zheng, X. Zhu, X. Lu, Y. Zhao, J. Feng, W. Jia, Guohua Wang, F. Li, F. Jin, D. |
| author_facet | Zheng, X. Zhu, X. Lu, Y. Zhao, J. Feng, W. Jia, Guohua Wang, F. Li, F. Jin, D. |
| author_sort | Zheng, X. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2016 American Chemical Society. Optical imaging through the near-infrared (NIR) window provides deep penetration of light up to several centimeters into biological tissues. Capable of emitting 800 nm luminescence under 980 nm illumination, the recently developed upconversion nanoparticles (UCNPs) suggest a promising optical contrast agent for in vivo bioimaging. However, presently they require high-power lasers to excite when applied to small animals, leading to significant scattering background that limits the detection sensitivity as well as a detrimental thermal effect. In this work, we show that the time-gating approach implementing pulsed illumination from a NIR diode laser and time-delayed imaging synchronized via an optical chopper offers detection sensitivity more than 1 order of magnitude higher than the conventional approach using optical band-pass filters (S/N, 47321/6353 vs 5339/58), when imaging UCNPs injected into Kunming mice. The pulsed laser illumination (70 µs ON in 200 µs period) also reduces the overall thermal accumulation to 35% of that under the continuous-wave mode. Technical details are given on setting up the time-gating unit comprising an optical chopper, a pinhole, and a microscopy eyepiece. Being generally compatible with any camera, this provides a convenient and low cost solution to NIR animal imaging using UCNPs as well as other luminescent probes. (Figure Presented). |
| first_indexed | 2025-11-14T07:56:17Z |
| format | Journal Article |
| id | curtin-20.500.11937-25271 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:56:17Z |
| publishDate | 2016 |
| publisher | American Chemical Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-252712018-03-29T09:08:51Z High-Contrast Visualization of Upconversion Luminescence in Mice Using Time-Gating Approach Zheng, X. Zhu, X. Lu, Y. Zhao, J. Feng, W. Jia, Guohua Wang, F. Li, F. Jin, D. © 2016 American Chemical Society. Optical imaging through the near-infrared (NIR) window provides deep penetration of light up to several centimeters into biological tissues. Capable of emitting 800 nm luminescence under 980 nm illumination, the recently developed upconversion nanoparticles (UCNPs) suggest a promising optical contrast agent for in vivo bioimaging. However, presently they require high-power lasers to excite when applied to small animals, leading to significant scattering background that limits the detection sensitivity as well as a detrimental thermal effect. In this work, we show that the time-gating approach implementing pulsed illumination from a NIR diode laser and time-delayed imaging synchronized via an optical chopper offers detection sensitivity more than 1 order of magnitude higher than the conventional approach using optical band-pass filters (S/N, 47321/6353 vs 5339/58), when imaging UCNPs injected into Kunming mice. The pulsed laser illumination (70 µs ON in 200 µs period) also reduces the overall thermal accumulation to 35% of that under the continuous-wave mode. Technical details are given on setting up the time-gating unit comprising an optical chopper, a pinhole, and a microscopy eyepiece. Being generally compatible with any camera, this provides a convenient and low cost solution to NIR animal imaging using UCNPs as well as other luminescent probes. (Figure Presented). 2016 Journal Article http://hdl.handle.net/20.500.11937/25271 10.1021/acs.analchem.5b04626 American Chemical Society restricted |
| spellingShingle | Zheng, X. Zhu, X. Lu, Y. Zhao, J. Feng, W. Jia, Guohua Wang, F. Li, F. Jin, D. High-Contrast Visualization of Upconversion Luminescence in Mice Using Time-Gating Approach |
| title | High-Contrast Visualization of Upconversion Luminescence in Mice Using Time-Gating Approach |
| title_full | High-Contrast Visualization of Upconversion Luminescence in Mice Using Time-Gating Approach |
| title_fullStr | High-Contrast Visualization of Upconversion Luminescence in Mice Using Time-Gating Approach |
| title_full_unstemmed | High-Contrast Visualization of Upconversion Luminescence in Mice Using Time-Gating Approach |
| title_short | High-Contrast Visualization of Upconversion Luminescence in Mice Using Time-Gating Approach |
| title_sort | high-contrast visualization of upconversion luminescence in mice using time-gating approach |
| url | http://hdl.handle.net/20.500.11937/25271 |