Characterization of Skeletonema costatum intracellular organic matter and study of nanomechanical properties under different solution conditions
In the current investigation, a rigorous characterization of the high molecular weight (HMW) compounds of Skeletonema costatum (SKC) intracellular organic matter (IOM), including nanomechanical properties, was conducted. HMW SKC-IOM was characterized as a mixture of polysaccharides, proteins, and li...
| Main Authors: | , , , , |
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| Format: | Journal Article |
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Elsevier BV
2016
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| Online Access: | http://hdl.handle.net/20.500.11937/25111 |
| _version_ | 1848751616898891776 |
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| author | Gutierrez Garces, Leonardo Aubry, C. Dramas, L. Aimar, P. Croue, J. |
| author_facet | Gutierrez Garces, Leonardo Aubry, C. Dramas, L. Aimar, P. Croue, J. |
| author_sort | Gutierrez Garces, Leonardo |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | In the current investigation, a rigorous characterization of the high molecular weight (HMW) compounds of Skeletonema costatum (SKC) intracellular organic matter (IOM), including nanomechanical properties, was conducted. HMW SKC-IOM was characterized as a mixture of polysaccharides, proteins, and lipids. Atomic force microscopy (AFM) provided crucial information of this isolate at a nanoscale resolution. HMW SKC-IOM showed highly responsive to solution chemistry: fully extended chains at low ionic strength, and compressing structures with increasing electrolyte concentration in solution. Interestingly, two regions of different nanomechanical properties were observed: (a) Region #1: located farther from the substrate and showing extended polymeric chains, and (b) Region #2: located <10 nm above the substrate and presenting compressed structures. The polymer length, polymer grafting density, and compressibility of these two regions were highly influenced by solution conditions. Results suggest that steric interactions originating from HMW SKC-IOM polymeric structure would be a dominant interacting mechanism with surfaces. The current investigation has successfully applied models of polymer physics to describe the complex HMW SKC-IOM structural conformation at different solution conditions. The detailed methodology presented provides a tool to characterize and understand biopolymers interactions with surfaces, including filtration membranes, and can be extended to other environmentally relevant organic compounds. |
| first_indexed | 2025-11-14T07:55:34Z |
| format | Journal Article |
| id | curtin-20.500.11937-25111 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:55:34Z |
| publishDate | 2016 |
| publisher | Elsevier BV |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-251112017-09-13T15:20:55Z Characterization of Skeletonema costatum intracellular organic matter and study of nanomechanical properties under different solution conditions Gutierrez Garces, Leonardo Aubry, C. Dramas, L. Aimar, P. Croue, J. In the current investigation, a rigorous characterization of the high molecular weight (HMW) compounds of Skeletonema costatum (SKC) intracellular organic matter (IOM), including nanomechanical properties, was conducted. HMW SKC-IOM was characterized as a mixture of polysaccharides, proteins, and lipids. Atomic force microscopy (AFM) provided crucial information of this isolate at a nanoscale resolution. HMW SKC-IOM showed highly responsive to solution chemistry: fully extended chains at low ionic strength, and compressing structures with increasing electrolyte concentration in solution. Interestingly, two regions of different nanomechanical properties were observed: (a) Region #1: located farther from the substrate and showing extended polymeric chains, and (b) Region #2: located <10 nm above the substrate and presenting compressed structures. The polymer length, polymer grafting density, and compressibility of these two regions were highly influenced by solution conditions. Results suggest that steric interactions originating from HMW SKC-IOM polymeric structure would be a dominant interacting mechanism with surfaces. The current investigation has successfully applied models of polymer physics to describe the complex HMW SKC-IOM structural conformation at different solution conditions. The detailed methodology presented provides a tool to characterize and understand biopolymers interactions with surfaces, including filtration membranes, and can be extended to other environmentally relevant organic compounds. 2016 Journal Article http://hdl.handle.net/20.500.11937/25111 10.1016/j.colsurfa.2016.06.025 Elsevier BV restricted |
| spellingShingle | Gutierrez Garces, Leonardo Aubry, C. Dramas, L. Aimar, P. Croue, J. Characterization of Skeletonema costatum intracellular organic matter and study of nanomechanical properties under different solution conditions |
| title | Characterization of Skeletonema costatum intracellular organic matter and study of nanomechanical properties under different solution conditions |
| title_full | Characterization of Skeletonema costatum intracellular organic matter and study of nanomechanical properties under different solution conditions |
| title_fullStr | Characterization of Skeletonema costatum intracellular organic matter and study of nanomechanical properties under different solution conditions |
| title_full_unstemmed | Characterization of Skeletonema costatum intracellular organic matter and study of nanomechanical properties under different solution conditions |
| title_short | Characterization of Skeletonema costatum intracellular organic matter and study of nanomechanical properties under different solution conditions |
| title_sort | characterization of skeletonema costatum intracellular organic matter and study of nanomechanical properties under different solution conditions |
| url | http://hdl.handle.net/20.500.11937/25111 |