Inheritance and alteration of transforming DNA during an induced parasexual cycle in the imperfect fungus Cladosporium fulvum
Protoplasts of a pAN7-1-transformed isolated of Cladosporium fulvum race 4, harbouring a tandem duplication of the vector, and an untransformed race 5 isolate were fused, without selection for the presence of vector sequences. Fusion products were allowed to haploidize spontaneously. The inheritance...
| Main Authors: | , |
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| Format: | Journal Article |
| Published: |
1993
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| Online Access: | http://hdl.handle.net/20.500.11937/24883 |
| _version_ | 1848751552572948480 |
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| author | Arnau, J. Oliver, Richard |
| author_facet | Arnau, J. Oliver, Richard |
| author_sort | Arnau, J. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Protoplasts of a pAN7-1-transformed isolated of Cladosporium fulvum race 4, harbouring a tandem duplication of the vector, and an untransformed race 5 isolate were fused, without selection for the presence of vector sequences. Fusion products were allowed to haploidize spontaneously. The inheritance of pAN7-1 sequences and the expression of the hph gene was studied in 85 progeny. A very high proportion (80%) of the progeny contained vector sequences and 70% of the progeny were resistant to hygromycin. Inactivation of the hph gene occurred in eight progeny. Rearrangement of vector sequences can account for the inactivation: there is no evidence for any RIP-like mechanism. A number of novel bands were observed. |
| first_indexed | 2025-11-14T07:54:33Z |
| format | Journal Article |
| id | curtin-20.500.11937-24883 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:54:33Z |
| publishDate | 1993 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-248832017-09-13T15:52:04Z Inheritance and alteration of transforming DNA during an induced parasexual cycle in the imperfect fungus Cladosporium fulvum Arnau, J. Oliver, Richard Protoplasts of a pAN7-1-transformed isolated of Cladosporium fulvum race 4, harbouring a tandem duplication of the vector, and an untransformed race 5 isolate were fused, without selection for the presence of vector sequences. Fusion products were allowed to haploidize spontaneously. The inheritance of pAN7-1 sequences and the expression of the hph gene was studied in 85 progeny. A very high proportion (80%) of the progeny contained vector sequences and 70% of the progeny were resistant to hygromycin. Inactivation of the hph gene occurred in eight progeny. Rearrangement of vector sequences can account for the inactivation: there is no evidence for any RIP-like mechanism. A number of novel bands were observed. 1993 Journal Article http://hdl.handle.net/20.500.11937/24883 10.1007/BF00312643 restricted |
| spellingShingle | Arnau, J. Oliver, Richard Inheritance and alteration of transforming DNA during an induced parasexual cycle in the imperfect fungus Cladosporium fulvum |
| title | Inheritance and alteration of transforming DNA during an induced parasexual cycle in the imperfect fungus Cladosporium fulvum |
| title_full | Inheritance and alteration of transforming DNA during an induced parasexual cycle in the imperfect fungus Cladosporium fulvum |
| title_fullStr | Inheritance and alteration of transforming DNA during an induced parasexual cycle in the imperfect fungus Cladosporium fulvum |
| title_full_unstemmed | Inheritance and alteration of transforming DNA during an induced parasexual cycle in the imperfect fungus Cladosporium fulvum |
| title_short | Inheritance and alteration of transforming DNA during an induced parasexual cycle in the imperfect fungus Cladosporium fulvum |
| title_sort | inheritance and alteration of transforming dna during an induced parasexual cycle in the imperfect fungus cladosporium fulvum |
| url | http://hdl.handle.net/20.500.11937/24883 |