Bioavailability and metabolism of orange juice flavanones in humans: Impact of a full-fat yogurt
The bioavailability of dietary phytochemicals may be influenced by the food matrix in which they are consumed. In this study the impact of a full-fat yogurt on the bioavailability and metabolism of orange juice flavanones was investigated. Human plasma and urine were collected over a 24 h period aft...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Published: |
American Chemical Society
2008
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| Online Access: | http://hdl.handle.net/20.500.11937/24736 |
| _version_ | 1848751511972085760 |
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| author | Mullen, W. Archeveque, M. Edwards, Christine Matsumoto, H. Crozier, A. |
| author_facet | Mullen, W. Archeveque, M. Edwards, Christine Matsumoto, H. Crozier, A. |
| author_sort | Mullen, W. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The bioavailability of dietary phytochemicals may be influenced by the food matrix in which they are consumed. In this study the impact of a full-fat yogurt on the bioavailability and metabolism of orange juice flavanones was investigated. Human plasma and urine were collected over a 24 h period after the consumption of 250 mL of orange juice containing a total of 168 µmol of hesperetin-7-O-rutinoside and 12 µmol of naringenin-7-O-rutinoside, with and without 150 mL of full-fat yogurt. The juice also contained 1 g of paracetamol and 5 g of lactulose. HPLC-MS2 analysis revealed the accumulation of hesperetin-7-O-glucuronide, and an unassigned hesperetin-O-glucuronide metabolite in plasma reached a peak concentration (Cmax) of 924 ± 224 nmol/L, 4.4 ± 0.5 h (Tmax) after orange juice ingestion. The Tmax is indicative of absorption in the colon. When the juice was consumed with yogurt, neither the Cmax at 661 ± 170 nmol/L nor the Tmax at 5.1 ± 0.4 h were significantly different from those obtained with juice alone. The two hesperetin glucuronides were also excreted in urine along with a third hesperetin-O-glucuronide, two hesperetin-O-glucuronide-O-sulfates, a hesperetin-O-diglucuronide, a naringenin-O-diglucuronide, and, tentatively identified, naringenin-7-O- glucuronide and naringenin-4'-O-glucuronide. This indicates the occurrence of substantial, postabsorption, phase II metabolism prior to urinary excretion. The quantity of flavanone metabolites excreted 0-5 h after orange juice ingestion was significantly reduced by yogurt, but over the full 0-24 h urine collection period, the amounts excreted, corresponding to ca. 7.0% of intake, were not affected by the addition of yogurt to the drink. Nor did yogurt have a significant effect on gastric emptying, as determined by plasma paracetamol levels, or on the mouth to cecum transit time of the head of the meal, assessed by measurement of lactulose-derived breath hydrogen. There is also a discussion of the merits of studies of the absorption and metabolism of flavanones based on direct analysis of metabolites by HPLC-MS and the more traditional indirect approach where samples are treated with a mollusc glucuronidase/sulfatase preparation prior to HPLC analysis of the released aglycones. © 2008 American Chemical Society. |
| first_indexed | 2025-11-14T07:53:54Z |
| format | Journal Article |
| id | curtin-20.500.11937-24736 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:53:54Z |
| publishDate | 2008 |
| publisher | American Chemical Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-247362017-09-13T15:13:57Z Bioavailability and metabolism of orange juice flavanones in humans: Impact of a full-fat yogurt Mullen, W. Archeveque, M. Edwards, Christine Matsumoto, H. Crozier, A. The bioavailability of dietary phytochemicals may be influenced by the food matrix in which they are consumed. In this study the impact of a full-fat yogurt on the bioavailability and metabolism of orange juice flavanones was investigated. Human plasma and urine were collected over a 24 h period after the consumption of 250 mL of orange juice containing a total of 168 µmol of hesperetin-7-O-rutinoside and 12 µmol of naringenin-7-O-rutinoside, with and without 150 mL of full-fat yogurt. The juice also contained 1 g of paracetamol and 5 g of lactulose. HPLC-MS2 analysis revealed the accumulation of hesperetin-7-O-glucuronide, and an unassigned hesperetin-O-glucuronide metabolite in plasma reached a peak concentration (Cmax) of 924 ± 224 nmol/L, 4.4 ± 0.5 h (Tmax) after orange juice ingestion. The Tmax is indicative of absorption in the colon. When the juice was consumed with yogurt, neither the Cmax at 661 ± 170 nmol/L nor the Tmax at 5.1 ± 0.4 h were significantly different from those obtained with juice alone. The two hesperetin glucuronides were also excreted in urine along with a third hesperetin-O-glucuronide, two hesperetin-O-glucuronide-O-sulfates, a hesperetin-O-diglucuronide, a naringenin-O-diglucuronide, and, tentatively identified, naringenin-7-O- glucuronide and naringenin-4'-O-glucuronide. This indicates the occurrence of substantial, postabsorption, phase II metabolism prior to urinary excretion. The quantity of flavanone metabolites excreted 0-5 h after orange juice ingestion was significantly reduced by yogurt, but over the full 0-24 h urine collection period, the amounts excreted, corresponding to ca. 7.0% of intake, were not affected by the addition of yogurt to the drink. Nor did yogurt have a significant effect on gastric emptying, as determined by plasma paracetamol levels, or on the mouth to cecum transit time of the head of the meal, assessed by measurement of lactulose-derived breath hydrogen. There is also a discussion of the merits of studies of the absorption and metabolism of flavanones based on direct analysis of metabolites by HPLC-MS and the more traditional indirect approach where samples are treated with a mollusc glucuronidase/sulfatase preparation prior to HPLC analysis of the released aglycones. © 2008 American Chemical Society. 2008 Journal Article http://hdl.handle.net/20.500.11937/24736 10.1021/jf801974v American Chemical Society restricted |
| spellingShingle | Mullen, W. Archeveque, M. Edwards, Christine Matsumoto, H. Crozier, A. Bioavailability and metabolism of orange juice flavanones in humans: Impact of a full-fat yogurt |
| title | Bioavailability and metabolism of orange juice flavanones in humans: Impact of a full-fat yogurt |
| title_full | Bioavailability and metabolism of orange juice flavanones in humans: Impact of a full-fat yogurt |
| title_fullStr | Bioavailability and metabolism of orange juice flavanones in humans: Impact of a full-fat yogurt |
| title_full_unstemmed | Bioavailability and metabolism of orange juice flavanones in humans: Impact of a full-fat yogurt |
| title_short | Bioavailability and metabolism of orange juice flavanones in humans: Impact of a full-fat yogurt |
| title_sort | bioavailability and metabolism of orange juice flavanones in humans: impact of a full-fat yogurt |
| url | http://hdl.handle.net/20.500.11937/24736 |