Isolation and foaming functionality of acid-soluble protein from lupin (Lupinus angustifolius) kernels
Background: Australian sweet lupin (ASL) protein is conventionally isolated by alkaline extraction/acid precipitation, leaving a waste stream containing acid-soluble proteins (ASPs) and contaminating raffinose family oligosaccharides (RFOs). The foaming functionality of ASP isolated from ASL is not...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Published: |
John Wiley and Sons Inc.
2013
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| Online Access: | http://hdl.handle.net/20.500.11937/24653 |
| _version_ | 1848751490487812096 |
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| author | Wong, A. Pitts, K. Jayasena, Vijay Johnson, Stuart |
| author_facet | Wong, A. Pitts, K. Jayasena, Vijay Johnson, Stuart |
| author_sort | Wong, A. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Background: Australian sweet lupin (ASL) protein is conventionally isolated by alkaline extraction/acid precipitation, leaving a waste stream containing acid-soluble proteins (ASPs) and contaminating raffinose family oligosaccharides (RFOs). The foaming functionality of ASP isolated from ASL is not known, but ASP from another lupin species has demonstrated high foaming functionality. Results: Pre-soaking ASL kernels increased their protein/RFO ratio; however, some protein was lost by soaking. The foaming capacity of ASL protein isolated by different methods was ranked in the following order: alkaline extraction/isoelectric precipitation < direct acid extraction (novel ASP) < supernatant from isoelectric precipitation (conventional ASP) < ultrafiltered novel ASP = fresh egg white. Electrophoresis indicated enrichment of y-conglutin and albumin peptides in ASPs and of a single peptide in the fibre residue from alkaline extraction. Conclusion: The high foaming capacity of < ultrafiltered novel ASP, similar to that of fresh egg white, indicates the potential of this lupin protein as a food ingredient for foaming applications. |
| first_indexed | 2025-11-14T07:53:33Z |
| format | Journal Article |
| id | curtin-20.500.11937-24653 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:53:33Z |
| publishDate | 2013 |
| publisher | John Wiley and Sons Inc. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-246532017-09-13T15:11:56Z Isolation and foaming functionality of acid-soluble protein from lupin (Lupinus angustifolius) kernels Wong, A. Pitts, K. Jayasena, Vijay Johnson, Stuart foaming oligosaccharides protein lupin dietary fibre ultrafiltration Background: Australian sweet lupin (ASL) protein is conventionally isolated by alkaline extraction/acid precipitation, leaving a waste stream containing acid-soluble proteins (ASPs) and contaminating raffinose family oligosaccharides (RFOs). The foaming functionality of ASP isolated from ASL is not known, but ASP from another lupin species has demonstrated high foaming functionality. Results: Pre-soaking ASL kernels increased their protein/RFO ratio; however, some protein was lost by soaking. The foaming capacity of ASL protein isolated by different methods was ranked in the following order: alkaline extraction/isoelectric precipitation < direct acid extraction (novel ASP) < supernatant from isoelectric precipitation (conventional ASP) < ultrafiltered novel ASP = fresh egg white. Electrophoresis indicated enrichment of y-conglutin and albumin peptides in ASPs and of a single peptide in the fibre residue from alkaline extraction. Conclusion: The high foaming capacity of < ultrafiltered novel ASP, similar to that of fresh egg white, indicates the potential of this lupin protein as a food ingredient for foaming applications. 2013 Journal Article http://hdl.handle.net/20.500.11937/24653 10.1002/jsfa.6249 John Wiley and Sons Inc. restricted |
| spellingShingle | foaming oligosaccharides protein lupin dietary fibre ultrafiltration Wong, A. Pitts, K. Jayasena, Vijay Johnson, Stuart Isolation and foaming functionality of acid-soluble protein from lupin (Lupinus angustifolius) kernels |
| title | Isolation and foaming functionality of acid-soluble protein from lupin (Lupinus angustifolius) kernels |
| title_full | Isolation and foaming functionality of acid-soluble protein from lupin (Lupinus angustifolius) kernels |
| title_fullStr | Isolation and foaming functionality of acid-soluble protein from lupin (Lupinus angustifolius) kernels |
| title_full_unstemmed | Isolation and foaming functionality of acid-soluble protein from lupin (Lupinus angustifolius) kernels |
| title_short | Isolation and foaming functionality of acid-soluble protein from lupin (Lupinus angustifolius) kernels |
| title_sort | isolation and foaming functionality of acid-soluble protein from lupin (lupinus angustifolius) kernels |
| topic | foaming oligosaccharides protein lupin dietary fibre ultrafiltration |
| url | http://hdl.handle.net/20.500.11937/24653 |