Subtilomycin: A New Lantibiotic from Bacillus subtilis Strain MMA7 Isolated from the Marine Sponge Haliclona simulans

Bacteriocins are attracting increased attention as an alternative to classic antibiotics in the fight against infectious disease and multidrug resistant pathogens. Bacillus subtilis strain MMA7 isolated from the marine sponge Haliclona simulans displays a broad spectrum antimicrobial activity, which...

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Main Authors: Phelan, Robert, Barret, Matthieu, Cotter, Paul, O’Connor, Paula, Chen, Rui, Morrissey, John, Dobson, Alan, O'Gara, Fergal, Barbosa, Teresa
Format: Journal Article
Published: MDPI Community & Outreach 2013
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/23902
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author Phelan, Robert
Barret, Matthieu
Cotter, Paul
O’Connor, Paula
Chen, Rui
Morrissey, John
Dobson, Alan
O'Gara, Fergal
Barbosa, Teresa
author_facet Phelan, Robert
Barret, Matthieu
Cotter, Paul
O’Connor, Paula
Chen, Rui
Morrissey, John
Dobson, Alan
O'Gara, Fergal
Barbosa, Teresa
author_sort Phelan, Robert
building Curtin Institutional Repository
collection Online Access
description Bacteriocins are attracting increased attention as an alternative to classic antibiotics in the fight against infectious disease and multidrug resistant pathogens. Bacillus subtilis strain MMA7 isolated from the marine sponge Haliclona simulans displays a broad spectrum antimicrobial activity, which includes Gram-positive and Gram-negative pathogens, as well as several pathogenic Candida species. This activity is in part associated with a newly identified lantibiotic, herein named as subtilomycin. The proposed biosynthetic cluster is composed of six genes, including protein-coding genes for LanB-like dehydratase and LanC-like cyclase modification enzymes, characteristic of the class I lantibiotics. The subtilomycin biosynthetic cluster in B. subtilis strain MMA7 is found in place of the sporulation killing factor (skf) operon, reported in many B. subtilis isolates and involved in a bacterial cannibalistic behaviour intended to delay sporulation. The presence of the subtilomycin biosynthetic cluster appears to be widespread amongst B. subtilis strains isolated from different shallow and deep water marine sponges. Subtilomycin possesses several desirable industrial and pharmaceutical physicochemical properties, including activity over a wide pH range, thermal resistance and water solubility. Additionally, the production of the lantibiotic subtilomycin could be a desirable property should B. subtilis strain MMA7 be employed as a probiotic in aquaculture applications.
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institution Curtin University Malaysia
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spelling curtin-20.500.11937-239022017-09-13T14:01:06Z Subtilomycin: A New Lantibiotic from Bacillus subtilis Strain MMA7 Isolated from the Marine Sponge Haliclona simulans Phelan, Robert Barret, Matthieu Cotter, Paul O’Connor, Paula Chen, Rui Morrissey, John Dobson, Alan O'Gara, Fergal Barbosa, Teresa subtilomycin Bacillus subtilis marine sponge lantibiotic antimicrobial Bacteriocins are attracting increased attention as an alternative to classic antibiotics in the fight against infectious disease and multidrug resistant pathogens. Bacillus subtilis strain MMA7 isolated from the marine sponge Haliclona simulans displays a broad spectrum antimicrobial activity, which includes Gram-positive and Gram-negative pathogens, as well as several pathogenic Candida species. This activity is in part associated with a newly identified lantibiotic, herein named as subtilomycin. The proposed biosynthetic cluster is composed of six genes, including protein-coding genes for LanB-like dehydratase and LanC-like cyclase modification enzymes, characteristic of the class I lantibiotics. The subtilomycin biosynthetic cluster in B. subtilis strain MMA7 is found in place of the sporulation killing factor (skf) operon, reported in many B. subtilis isolates and involved in a bacterial cannibalistic behaviour intended to delay sporulation. The presence of the subtilomycin biosynthetic cluster appears to be widespread amongst B. subtilis strains isolated from different shallow and deep water marine sponges. Subtilomycin possesses several desirable industrial and pharmaceutical physicochemical properties, including activity over a wide pH range, thermal resistance and water solubility. Additionally, the production of the lantibiotic subtilomycin could be a desirable property should B. subtilis strain MMA7 be employed as a probiotic in aquaculture applications. 2013 Journal Article http://hdl.handle.net/20.500.11937/23902 10.3390/md11061878 MDPI Community & Outreach unknown
spellingShingle subtilomycin
Bacillus subtilis
marine sponge
lantibiotic
antimicrobial
Phelan, Robert
Barret, Matthieu
Cotter, Paul
O’Connor, Paula
Chen, Rui
Morrissey, John
Dobson, Alan
O'Gara, Fergal
Barbosa, Teresa
Subtilomycin: A New Lantibiotic from Bacillus subtilis Strain MMA7 Isolated from the Marine Sponge Haliclona simulans
title Subtilomycin: A New Lantibiotic from Bacillus subtilis Strain MMA7 Isolated from the Marine Sponge Haliclona simulans
title_full Subtilomycin: A New Lantibiotic from Bacillus subtilis Strain MMA7 Isolated from the Marine Sponge Haliclona simulans
title_fullStr Subtilomycin: A New Lantibiotic from Bacillus subtilis Strain MMA7 Isolated from the Marine Sponge Haliclona simulans
title_full_unstemmed Subtilomycin: A New Lantibiotic from Bacillus subtilis Strain MMA7 Isolated from the Marine Sponge Haliclona simulans
title_short Subtilomycin: A New Lantibiotic from Bacillus subtilis Strain MMA7 Isolated from the Marine Sponge Haliclona simulans
title_sort subtilomycin: a new lantibiotic from bacillus subtilis strain mma7 isolated from the marine sponge haliclona simulans
topic subtilomycin
Bacillus subtilis
marine sponge
lantibiotic
antimicrobial
url http://hdl.handle.net/20.500.11937/23902