Novel Endorna-like viruses, including three with two open reading frames, challenge the membership criteria and taxonomy of the Endornaviridae
Viruses associated with wild orchids and their mycorrhizal fungi are poorly studied. Using a shotgun sequencing approach, we identified eight novel endornavirus-like genome sequences from isolates of Ceratobasidium fungi isolated from pelotons within root cortical cells of wild indigenous orchid spe...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
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Reed Elsevier
2016
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| Online Access: | http://hdl.handle.net/20.500.11937/23878 |
| _version_ | 1848751274330161152 |
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| author | Ong, J. Li, H. Sivasithamparam, K. Dixon, Kingsley Jones, M. Wylie, S. |
| author_facet | Ong, J. Li, H. Sivasithamparam, K. Dixon, Kingsley Jones, M. Wylie, S. |
| author_sort | Ong, J. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Viruses associated with wild orchids and their mycorrhizal fungi are poorly studied. Using a shotgun sequencing approach, we identified eight novel endornavirus-like genome sequences from isolates of Ceratobasidium fungi isolated from pelotons within root cortical cells of wild indigenous orchid species Microtis media, Pterostylis sanguinea and an undetermined species of Pterostylis in Western Australia. They represent the first endornaviruses to be described from orchid mycorrhizal fungi and from the Australian continent. Five of the novel endornaviruses were detected from one Ceratobasidium isolate collected from one Pterostylis plant. The partial and complete viral replicases shared low (9–30%) identities with one another and with endornaviruses described from elsewhere. Four had genome lengths greater than those of previously described endornaviruses, two resembled ascomycete-infecting endornaviruses, and unlike currently described endornaviruses, three had two open reading frames. The unusual features of these new viruses challenge current taxonomic criteria for membership of the family Endornaviridae. |
| first_indexed | 2025-11-14T07:50:07Z |
| format | Journal Article |
| id | curtin-20.500.11937-23878 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:50:07Z |
| publishDate | 2016 |
| publisher | Reed Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-238782017-09-13T14:00:15Z Novel Endorna-like viruses, including three with two open reading frames, challenge the membership criteria and taxonomy of the Endornaviridae Ong, J. Li, H. Sivasithamparam, K. Dixon, Kingsley Jones, M. Wylie, S. Viruses associated with wild orchids and their mycorrhizal fungi are poorly studied. Using a shotgun sequencing approach, we identified eight novel endornavirus-like genome sequences from isolates of Ceratobasidium fungi isolated from pelotons within root cortical cells of wild indigenous orchid species Microtis media, Pterostylis sanguinea and an undetermined species of Pterostylis in Western Australia. They represent the first endornaviruses to be described from orchid mycorrhizal fungi and from the Australian continent. Five of the novel endornaviruses were detected from one Ceratobasidium isolate collected from one Pterostylis plant. The partial and complete viral replicases shared low (9–30%) identities with one another and with endornaviruses described from elsewhere. Four had genome lengths greater than those of previously described endornaviruses, two resembled ascomycete-infecting endornaviruses, and unlike currently described endornaviruses, three had two open reading frames. The unusual features of these new viruses challenge current taxonomic criteria for membership of the family Endornaviridae. 2016 Journal Article http://hdl.handle.net/20.500.11937/23878 10.1016/j.virol.2016.08.019 Reed Elsevier restricted |
| spellingShingle | Ong, J. Li, H. Sivasithamparam, K. Dixon, Kingsley Jones, M. Wylie, S. Novel Endorna-like viruses, including three with two open reading frames, challenge the membership criteria and taxonomy of the Endornaviridae |
| title | Novel Endorna-like viruses, including three with two open reading frames, challenge the membership criteria and taxonomy of the Endornaviridae |
| title_full | Novel Endorna-like viruses, including three with two open reading frames, challenge the membership criteria and taxonomy of the Endornaviridae |
| title_fullStr | Novel Endorna-like viruses, including three with two open reading frames, challenge the membership criteria and taxonomy of the Endornaviridae |
| title_full_unstemmed | Novel Endorna-like viruses, including three with two open reading frames, challenge the membership criteria and taxonomy of the Endornaviridae |
| title_short | Novel Endorna-like viruses, including three with two open reading frames, challenge the membership criteria and taxonomy of the Endornaviridae |
| title_sort | novel endorna-like viruses, including three with two open reading frames, challenge the membership criteria and taxonomy of the endornaviridae |
| url | http://hdl.handle.net/20.500.11937/23878 |