The bacterial secondary metabolite 2,4-diacetylphloroglucinol impairs mitochondrial function and affects calcium homeostasis in Neurospora crassa
The bacterial secondary metabolite 2,4-diacetylphloroglucinol (DAPG) is of interest as an active ingredient of biological control strains of Pseudomonas fluorescens and as a potential lead pharmaceutical molecule because of its capacity to inhibit growth of diverse microbial and non-microbial cells....
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
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Academic Press
2013
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| Online Access: | http://hdl.handle.net/20.500.11937/23703 |
| _version_ | 1848751223562305536 |
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| author | Troppens, Danielle Chu, Meiling Holcombe, Lucy Gleeson, Olive O'Gara, Fergal Read, Nick Morrissey, John |
| author_facet | Troppens, Danielle Chu, Meiling Holcombe, Lucy Gleeson, Olive O'Gara, Fergal Read, Nick Morrissey, John |
| author_sort | Troppens, Danielle |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The bacterial secondary metabolite 2,4-diacetylphloroglucinol (DAPG) is of interest as an active ingredient of biological control strains of Pseudomonas fluorescens and as a potential lead pharmaceutical molecule because of its capacity to inhibit growth of diverse microbial and non-microbial cells. The mechanism by which this occurs is unknown and in this study the filamentous fungus Neurospora crassa was used as a model to investigate the effects of DAPG on a eukaryotic cell. Colony growth, conidial germination and cell fusion assays confirmed the inhibitory nature of DAPG towards N. crassa. A number of different fluorescent dyes and fluorescent protein reporters were used to assess the effects of DAPG treatment on mitochondrial and other cellular functions. DAPG treatment led to changes in mitochondrial morphology, and rapid loss of mitochondrial membrane potential. These effects are likely to be responsible for the toxicity of DAPG. It was also found that DAPG treatment caused extracellular calcium to be taken up by conidial germlings leading to a transient increase in cytosolic free Ca2+ with a distinct concentration dependent Ca2+ signature. |
| first_indexed | 2025-11-14T07:49:19Z |
| format | Journal Article |
| id | curtin-20.500.11937-23703 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:49:19Z |
| publishDate | 2013 |
| publisher | Academic Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-237032017-09-13T13:59:02Z The bacterial secondary metabolite 2,4-diacetylphloroglucinol impairs mitochondrial function and affects calcium homeostasis in Neurospora crassa Troppens, Danielle Chu, Meiling Holcombe, Lucy Gleeson, Olive O'Gara, Fergal Read, Nick Morrissey, John Neurospora crassa Calcium Mitochondria DAPG Antimicrobial The bacterial secondary metabolite 2,4-diacetylphloroglucinol (DAPG) is of interest as an active ingredient of biological control strains of Pseudomonas fluorescens and as a potential lead pharmaceutical molecule because of its capacity to inhibit growth of diverse microbial and non-microbial cells. The mechanism by which this occurs is unknown and in this study the filamentous fungus Neurospora crassa was used as a model to investigate the effects of DAPG on a eukaryotic cell. Colony growth, conidial germination and cell fusion assays confirmed the inhibitory nature of DAPG towards N. crassa. A number of different fluorescent dyes and fluorescent protein reporters were used to assess the effects of DAPG treatment on mitochondrial and other cellular functions. DAPG treatment led to changes in mitochondrial morphology, and rapid loss of mitochondrial membrane potential. These effects are likely to be responsible for the toxicity of DAPG. It was also found that DAPG treatment caused extracellular calcium to be taken up by conidial germlings leading to a transient increase in cytosolic free Ca2+ with a distinct concentration dependent Ca2+ signature. 2013 Journal Article http://hdl.handle.net/20.500.11937/23703 10.1016/j.fgb.2013.04.006 Academic Press restricted |
| spellingShingle | Neurospora crassa Calcium Mitochondria DAPG Antimicrobial Troppens, Danielle Chu, Meiling Holcombe, Lucy Gleeson, Olive O'Gara, Fergal Read, Nick Morrissey, John The bacterial secondary metabolite 2,4-diacetylphloroglucinol impairs mitochondrial function and affects calcium homeostasis in Neurospora crassa |
| title | The bacterial secondary metabolite 2,4-diacetylphloroglucinol impairs mitochondrial function and affects calcium homeostasis in Neurospora crassa |
| title_full | The bacterial secondary metabolite 2,4-diacetylphloroglucinol impairs mitochondrial function and affects calcium homeostasis in Neurospora crassa |
| title_fullStr | The bacterial secondary metabolite 2,4-diacetylphloroglucinol impairs mitochondrial function and affects calcium homeostasis in Neurospora crassa |
| title_full_unstemmed | The bacterial secondary metabolite 2,4-diacetylphloroglucinol impairs mitochondrial function and affects calcium homeostasis in Neurospora crassa |
| title_short | The bacterial secondary metabolite 2,4-diacetylphloroglucinol impairs mitochondrial function and affects calcium homeostasis in Neurospora crassa |
| title_sort | bacterial secondary metabolite 2,4-diacetylphloroglucinol impairs mitochondrial function and affects calcium homeostasis in neurospora crassa |
| topic | Neurospora crassa Calcium Mitochondria DAPG Antimicrobial |
| url | http://hdl.handle.net/20.500.11937/23703 |