Metabolite profiling identifies the mycotoxin alternariol in the pathogen Stagonospora nodorum
A recent comparative proteomics study identified the short-chain dehydrogenase (Sch1) as being required for asexual sporulation (Tan et al. Eukaryotic Cell 7:1916–1929, 2008). Metabolite profiling was undertaken on the mutant strains of Stagonospora nodorum lacking the Sch1 gene to help elucidate it...
| Main Authors: | , , , , |
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| Format: | Journal Article |
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Springer Science+Business Media
2009
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| Online Access: | http://hdl.handle.net/20.500.11937/23652 |
| _version_ | 1848751209819668480 |
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| author | Tan, Kar-Chun Trengove, R. Maker, G. Oliver, Richard Solomon, P. |
| author_facet | Tan, Kar-Chun Trengove, R. Maker, G. Oliver, Richard Solomon, P. |
| author_sort | Tan, Kar-Chun |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | A recent comparative proteomics study identified the short-chain dehydrogenase (Sch1) as being required for asexual sporulation (Tan et al. Eukaryotic Cell 7:1916–1929, 2008). Metabolite profiling was undertaken on the mutant strains of Stagonospora nodorum lacking the Sch1 gene to help elucidate its role. Gas chromatography-mass spectrometry of the polar metabolites in the Sch1 mutants identified a secondary metabolite at a 200-fold greater concentration than observed in the wild-type strains. Comparative analysis of the secondary metabolite and the mycotoxin alternariol using ESI-MS/MS confirmed the identity of the compound as alternariol. This is the first report to confirm the presence of a mycotoxin in S. nodorum and compelling the field to consider the health implication of this disease. |
| first_indexed | 2025-11-14T07:49:06Z |
| format | Journal Article |
| id | curtin-20.500.11937-23652 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:49:06Z |
| publishDate | 2009 |
| publisher | Springer Science+Business Media |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-236522017-09-13T16:03:35Z Metabolite profiling identifies the mycotoxin alternariol in the pathogen Stagonospora nodorum Tan, Kar-Chun Trengove, R. Maker, G. Oliver, Richard Solomon, P. Pathogen Stagonospora nodorum Mycotoxin Alternariol Metabolomics A recent comparative proteomics study identified the short-chain dehydrogenase (Sch1) as being required for asexual sporulation (Tan et al. Eukaryotic Cell 7:1916–1929, 2008). Metabolite profiling was undertaken on the mutant strains of Stagonospora nodorum lacking the Sch1 gene to help elucidate its role. Gas chromatography-mass spectrometry of the polar metabolites in the Sch1 mutants identified a secondary metabolite at a 200-fold greater concentration than observed in the wild-type strains. Comparative analysis of the secondary metabolite and the mycotoxin alternariol using ESI-MS/MS confirmed the identity of the compound as alternariol. This is the first report to confirm the presence of a mycotoxin in S. nodorum and compelling the field to consider the health implication of this disease. 2009 Journal Article http://hdl.handle.net/20.500.11937/23652 10.1007/s11306-009-0158-2 Springer Science+Business Media fulltext |
| spellingShingle | Pathogen Stagonospora nodorum Mycotoxin Alternariol Metabolomics Tan, Kar-Chun Trengove, R. Maker, G. Oliver, Richard Solomon, P. Metabolite profiling identifies the mycotoxin alternariol in the pathogen Stagonospora nodorum |
| title | Metabolite profiling identifies the mycotoxin alternariol in the pathogen Stagonospora nodorum |
| title_full | Metabolite profiling identifies the mycotoxin alternariol in the pathogen Stagonospora nodorum |
| title_fullStr | Metabolite profiling identifies the mycotoxin alternariol in the pathogen Stagonospora nodorum |
| title_full_unstemmed | Metabolite profiling identifies the mycotoxin alternariol in the pathogen Stagonospora nodorum |
| title_short | Metabolite profiling identifies the mycotoxin alternariol in the pathogen Stagonospora nodorum |
| title_sort | metabolite profiling identifies the mycotoxin alternariol in the pathogen stagonospora nodorum |
| topic | Pathogen Stagonospora nodorum Mycotoxin Alternariol Metabolomics |
| url | http://hdl.handle.net/20.500.11937/23652 |