Modelling and numerical simulation of liquid–solid circulating fluidized bed system for protein purification
A novel liquid–solid circulating fluidized bed (LSCFB) was modelled for protein recovery from the feed broth. A typical LSCFB system consists of downer and riser, integrating two different operations simultaneously. A general purpose, extensible, and dynamic model was written based on the tanks-in-s...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
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Institute of Chemical Engineers (IchemE), Elsevier B.V.
2013
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/23632 |
| _version_ | 1848751204111220736 |
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| author | Pei, Wen Utikar, Ranjeet Pareek, Vishnu Johnson, Stuart Kale, S. Lali, A. |
| author_facet | Pei, Wen Utikar, Ranjeet Pareek, Vishnu Johnson, Stuart Kale, S. Lali, A. |
| author_sort | Pei, Wen |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | A novel liquid–solid circulating fluidized bed (LSCFB) was modelled for protein recovery from the feed broth. A typical LSCFB system consists of downer and riser, integrating two different operations simultaneously. A general purpose, extensible, and dynamic model was written based on the tanks-in-series framework. The model allowed adjusting the degree of backmixing in each phase for both columns. The model was validated with previously published data on extraction of bovine serum albumin (BSA) as model protein. Detailed dynamic analysis was performed on the protein recovery operation. The interaction between the riser and downer were captured. Parametric studies on protein recovery in LSCFB system were carried out using the validated model to better understand the system behaviour. Simulation results have shown that both production rate and overall recovery increased with solids circulation rate, superficial liquid velocity in the downer and riser, and feed solution concentration. The model was flexible and could use various forms of ion exchange kinetics and could simulate different hydrodynamic behaviours. It was useful to gain insight into protein recovery processes. The general nature of the model made it useful to study other protein recovery operations for plant and animal proteins. It could also be useful for further multi-objective optimization studies to optimize the LSCFB system. |
| first_indexed | 2025-11-14T07:49:00Z |
| format | Journal Article |
| id | curtin-20.500.11937-23632 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:49:00Z |
| publishDate | 2013 |
| publisher | Institute of Chemical Engineers (IchemE), Elsevier B.V. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-236322017-09-13T15:57:06Z Modelling and numerical simulation of liquid–solid circulating fluidized bed system for protein purification Pei, Wen Utikar, Ranjeet Pareek, Vishnu Johnson, Stuart Kale, S. Lali, A. dynamic modelling dynamic simulation protein recovery liquid–solid circulating fluidized bed (LSCFB) adsorption tanks-in-series A novel liquid–solid circulating fluidized bed (LSCFB) was modelled for protein recovery from the feed broth. A typical LSCFB system consists of downer and riser, integrating two different operations simultaneously. A general purpose, extensible, and dynamic model was written based on the tanks-in-series framework. The model allowed adjusting the degree of backmixing in each phase for both columns. The model was validated with previously published data on extraction of bovine serum albumin (BSA) as model protein. Detailed dynamic analysis was performed on the protein recovery operation. The interaction between the riser and downer were captured. Parametric studies on protein recovery in LSCFB system were carried out using the validated model to better understand the system behaviour. Simulation results have shown that both production rate and overall recovery increased with solids circulation rate, superficial liquid velocity in the downer and riser, and feed solution concentration. The model was flexible and could use various forms of ion exchange kinetics and could simulate different hydrodynamic behaviours. It was useful to gain insight into protein recovery processes. The general nature of the model made it useful to study other protein recovery operations for plant and animal proteins. It could also be useful for further multi-objective optimization studies to optimize the LSCFB system. 2013 Journal Article http://hdl.handle.net/20.500.11937/23632 10.1016/j.cherd.2013.04.004 Institute of Chemical Engineers (IchemE), Elsevier B.V. fulltext |
| spellingShingle | dynamic modelling dynamic simulation protein recovery liquid–solid circulating fluidized bed (LSCFB) adsorption tanks-in-series Pei, Wen Utikar, Ranjeet Pareek, Vishnu Johnson, Stuart Kale, S. Lali, A. Modelling and numerical simulation of liquid–solid circulating fluidized bed system for protein purification |
| title | Modelling and numerical simulation of liquid–solid circulating fluidized bed system for protein purification |
| title_full | Modelling and numerical simulation of liquid–solid circulating fluidized bed system for protein purification |
| title_fullStr | Modelling and numerical simulation of liquid–solid circulating fluidized bed system for protein purification |
| title_full_unstemmed | Modelling and numerical simulation of liquid–solid circulating fluidized bed system for protein purification |
| title_short | Modelling and numerical simulation of liquid–solid circulating fluidized bed system for protein purification |
| title_sort | modelling and numerical simulation of liquid–solid circulating fluidized bed system for protein purification |
| topic | dynamic modelling dynamic simulation protein recovery liquid–solid circulating fluidized bed (LSCFB) adsorption tanks-in-series |
| url | http://hdl.handle.net/20.500.11937/23632 |