PCR assays in detecting methicillin resistance in staphylococci: Coagulase negative staphylococci (CNS), S. aureus, and MRSA with the PVL gene
Methicillin resistance, in particular hetero-methicillin resistance, in S aureus and the coagulase negative staphylococci (CNS) can be difficult to detect by phenotypic methods. Subsequently detection of the mecA gene for determining methicillin resistance in staphylococci is generally considered to...
| Main Authors: | , , , |
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| Format: | Book Chapter |
| Published: |
Springer Netherlands
2010
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| Online Access: | http://hdl.handle.net/20.500.11937/23359 |
| _version_ | 1848751128634720256 |
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| author | Merlino, J. Kay, I. Coombs, Geoffrey Palladino, S. |
| author_facet | Merlino, J. Kay, I. Coombs, Geoffrey Palladino, S. |
| author_sort | Merlino, J. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Methicillin resistance, in particular hetero-methicillin resistance, in S aureus and the coagulase negative staphylococci (CNS) can be difficult to detect by phenotypic methods. Subsequently detection of the mecA gene for determining methicillin resistance in staphylococci is generally considered to be the Gold Standard. In Australian diagnostic microbiology laboratories, molecular assays used for the detection of the mecA gene have generally been designed in-house. These assays have either been single primer PCR assays targeting the mecA gene or a duplex primer PCR assay with primers targeting the mecA gene and a gene specific for S. aureus such as the nuc (nuclease) or fem gene. In some laboratories a Panton Valentine-leukocidin (PVL) gene primer has been incorporated with the duplex PCR assay allowing the detection of PVL virulence genes in S aureus. © 2010 Springer Science+Business Media B.V. |
| first_indexed | 2025-11-14T07:47:48Z |
| format | Book Chapter |
| id | curtin-20.500.11937-23359 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:47:48Z |
| publishDate | 2010 |
| publisher | Springer Netherlands |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-233592017-09-13T13:58:22Z PCR assays in detecting methicillin resistance in staphylococci: Coagulase negative staphylococci (CNS), S. aureus, and MRSA with the PVL gene Merlino, J. Kay, I. Coombs, Geoffrey Palladino, S. Methicillin resistance, in particular hetero-methicillin resistance, in S aureus and the coagulase negative staphylococci (CNS) can be difficult to detect by phenotypic methods. Subsequently detection of the mecA gene for determining methicillin resistance in staphylococci is generally considered to be the Gold Standard. In Australian diagnostic microbiology laboratories, molecular assays used for the detection of the mecA gene have generally been designed in-house. These assays have either been single primer PCR assays targeting the mecA gene or a duplex primer PCR assay with primers targeting the mecA gene and a gene specific for S. aureus such as the nuc (nuclease) or fem gene. In some laboratories a Panton Valentine-leukocidin (PVL) gene primer has been incorporated with the duplex PCR assay allowing the detection of PVL virulence genes in S aureus. © 2010 Springer Science+Business Media B.V. 2010 Book Chapter http://hdl.handle.net/20.500.11937/23359 10.1007/978-90-481-9039-3_72 Springer Netherlands restricted |
| spellingShingle | Merlino, J. Kay, I. Coombs, Geoffrey Palladino, S. PCR assays in detecting methicillin resistance in staphylococci: Coagulase negative staphylococci (CNS), S. aureus, and MRSA with the PVL gene |
| title | PCR assays in detecting methicillin resistance in staphylococci: Coagulase negative staphylococci (CNS), S. aureus, and MRSA with the PVL gene |
| title_full | PCR assays in detecting methicillin resistance in staphylococci: Coagulase negative staphylococci (CNS), S. aureus, and MRSA with the PVL gene |
| title_fullStr | PCR assays in detecting methicillin resistance in staphylococci: Coagulase negative staphylococci (CNS), S. aureus, and MRSA with the PVL gene |
| title_full_unstemmed | PCR assays in detecting methicillin resistance in staphylococci: Coagulase negative staphylococci (CNS), S. aureus, and MRSA with the PVL gene |
| title_short | PCR assays in detecting methicillin resistance in staphylococci: Coagulase negative staphylococci (CNS), S. aureus, and MRSA with the PVL gene |
| title_sort | pcr assays in detecting methicillin resistance in staphylococci: coagulase negative staphylococci (cns), s. aureus, and mrsa with the pvl gene |
| url | http://hdl.handle.net/20.500.11937/23359 |