Cyclization enhances function of linear anti-arthritic peptides
This study describes the biophysical and immunomodulatory features of a cyclic peptide termed C1 which consists of alternating d-, l-amino acids and is capable of inhibiting IL-2 production in vitro and reducing the induction and extent of T-cell mediated inflammation in animal models. Solid-state n...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal Article |
| Published: |
Academic Press
2014
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/23307 |
| _version_ | 1848751114116136960 |
|---|---|
| author | Ali, Marina Amon, Michael Bender, Vera Bolte, Andrea Separovic, Frances Benson, Heather Manolios, Nicholas |
| author_facet | Ali, Marina Amon, Michael Bender, Vera Bolte, Andrea Separovic, Frances Benson, Heather Manolios, Nicholas |
| author_sort | Ali, Marina |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | This study describes the biophysical and immunomodulatory features of a cyclic peptide termed C1 which consists of alternating d-, l-amino acids and is capable of inhibiting IL-2 production in vitro and reducing the induction and extent of T-cell mediated inflammation in animal models. Solid-state nuclear magnetic resonance demonstrates that the peptide orders the lipid bilayer, suggesting a transmembrane orientation, and this is supported by surface plasmon resonance indicating strong binding affinity of C1 to model membranes. In vitro cell viability and proliferation assays show that C1 does not disrupt the integrity of cell surface membranes. Permeation studies of C1 and analogs across human epidermis cells show that the stability and skin permeability are enhanced by cyclization. Treatment with C1 in an asthma and in an arthritis animal model resulted in a suppressed immune response. Cyclization may be a useful means of enhancing biological linear peptide activity and improving delivery. |
| first_indexed | 2025-11-14T07:47:34Z |
| format | Journal Article |
| id | curtin-20.500.11937-23307 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:47:34Z |
| publishDate | 2014 |
| publisher | Academic Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-233072017-09-13T15:58:25Z Cyclization enhances function of linear anti-arthritic peptides Ali, Marina Amon, Michael Bender, Vera Bolte, Andrea Separovic, Frances Benson, Heather Manolios, Nicholas Arthritis T cells Asthma Peptides NMR Inflammation This study describes the biophysical and immunomodulatory features of a cyclic peptide termed C1 which consists of alternating d-, l-amino acids and is capable of inhibiting IL-2 production in vitro and reducing the induction and extent of T-cell mediated inflammation in animal models. Solid-state nuclear magnetic resonance demonstrates that the peptide orders the lipid bilayer, suggesting a transmembrane orientation, and this is supported by surface plasmon resonance indicating strong binding affinity of C1 to model membranes. In vitro cell viability and proliferation assays show that C1 does not disrupt the integrity of cell surface membranes. Permeation studies of C1 and analogs across human epidermis cells show that the stability and skin permeability are enhanced by cyclization. Treatment with C1 in an asthma and in an arthritis animal model resulted in a suppressed immune response. Cyclization may be a useful means of enhancing biological linear peptide activity and improving delivery. 2014 Journal Article http://hdl.handle.net/20.500.11937/23307 10.1016/j.clim.2013.10.005 Academic Press restricted |
| spellingShingle | Arthritis T cells Asthma Peptides NMR Inflammation Ali, Marina Amon, Michael Bender, Vera Bolte, Andrea Separovic, Frances Benson, Heather Manolios, Nicholas Cyclization enhances function of linear anti-arthritic peptides |
| title | Cyclization enhances function of linear anti-arthritic peptides |
| title_full | Cyclization enhances function of linear anti-arthritic peptides |
| title_fullStr | Cyclization enhances function of linear anti-arthritic peptides |
| title_full_unstemmed | Cyclization enhances function of linear anti-arthritic peptides |
| title_short | Cyclization enhances function of linear anti-arthritic peptides |
| title_sort | cyclization enhances function of linear anti-arthritic peptides |
| topic | Arthritis T cells Asthma Peptides NMR Inflammation |
| url | http://hdl.handle.net/20.500.11937/23307 |