Long-term RNA persistence in postmortem contexts

Ribonucleic acids (RNA) are generally considered fragile molecules that are readily degraded. However, there is growing documentation of long-term (from days to centuries) RNA persistence in a variety of contexts and tissue types, and as such a number of academic disciplines are beginning to exploit...

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Main Authors: Fordyce, S., Kampmann, M., L van Doom, N., Gilbert, Thomas
Format: Journal Article
Published: BioMed Central 2013
Subjects:
Online Access:http://www.biomedcentral.com/content/pdf/2041-2223-4-7.pdf
http://hdl.handle.net/20.500.11937/23277
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author Fordyce, S.
Kampmann, M.
L van Doom, N.
Gilbert, Thomas
author_facet Fordyce, S.
Kampmann, M.
L van Doom, N.
Gilbert, Thomas
author_sort Fordyce, S.
building Curtin Institutional Repository
collection Online Access
description Ribonucleic acids (RNA) are generally considered fragile molecules that are readily degraded. However, there is growing documentation of long-term (from days to centuries) RNA persistence in a variety of contexts and tissue types, and as such a number of academic disciplines are beginning to exploit degraded RNA. While the reasons for its survival are not fully understood, there are several plausible mechanisms that would safeguard this molecule against degradation. However, after examining the literature available on the postmortem instability and decay mechanisms of RNA, it has become clear that limited experimental studies and no reviews offer an overview of these mechanisms. Hence in this review we outline molecular reasons for RNA surviving long-term postmortem, and provide specific examples of RNA survival in forensic, archival and archaeological contexts. A better understanding of the mechanisms of RNA decay will be crucial for developing expectations on its long-term survival.
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spelling curtin-20.500.11937-232772017-02-28T01:37:24Z Long-term RNA persistence in postmortem contexts Fordyce, S. Kampmann, M. L van Doom, N. Gilbert, Thomas Paleogenetics Postmortem Forensic Ribonucleic acid (RNA) Instability Ribonucleic acids (RNA) are generally considered fragile molecules that are readily degraded. However, there is growing documentation of long-term (from days to centuries) RNA persistence in a variety of contexts and tissue types, and as such a number of academic disciplines are beginning to exploit degraded RNA. While the reasons for its survival are not fully understood, there are several plausible mechanisms that would safeguard this molecule against degradation. However, after examining the literature available on the postmortem instability and decay mechanisms of RNA, it has become clear that limited experimental studies and no reviews offer an overview of these mechanisms. Hence in this review we outline molecular reasons for RNA surviving long-term postmortem, and provide specific examples of RNA survival in forensic, archival and archaeological contexts. A better understanding of the mechanisms of RNA decay will be crucial for developing expectations on its long-term survival. 2013 Journal Article http://hdl.handle.net/20.500.11937/23277 http://www.biomedcentral.com/content/pdf/2041-2223-4-7.pdf BioMed Central restricted
spellingShingle Paleogenetics
Postmortem
Forensic
Ribonucleic acid (RNA)
Instability
Fordyce, S.
Kampmann, M.
L van Doom, N.
Gilbert, Thomas
Long-term RNA persistence in postmortem contexts
title Long-term RNA persistence in postmortem contexts
title_full Long-term RNA persistence in postmortem contexts
title_fullStr Long-term RNA persistence in postmortem contexts
title_full_unstemmed Long-term RNA persistence in postmortem contexts
title_short Long-term RNA persistence in postmortem contexts
title_sort long-term rna persistence in postmortem contexts
topic Paleogenetics
Postmortem
Forensic
Ribonucleic acid (RNA)
Instability
url http://www.biomedcentral.com/content/pdf/2041-2223-4-7.pdf
http://hdl.handle.net/20.500.11937/23277