Low seroprevalence of Murray Valley Encephalitis and Kunjin viruses in an opportunistic serosurvey, Victoria 2011

Objective: To assess evidence of recent and past exposure to Murray Valley encephalitis virus (MVEV) and West Nile clade Kunjin virus (KUNV) in residents of the Murray Valley, Victoria, during a period of demonstrated activity of both viruses in early 2011. Methods: A cross-sectional serosurvey usin...

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Main Authors: Williams, Stephanie, Richards, Jack, Faddy, Helen, Leydon, Jennie, Moran, Rodney, Nicholson, Suellen, Perry, Faye, Paskin, Roger, Catton, Mike, Lester, Rosemary, MacKenzie, John
Format: Journal Article
Published: Wiley-Blackwell Publishing Asia 2013
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/22924
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author Williams, Stephanie
Richards, Jack
Faddy, Helen
Leydon, Jennie
Moran, Rodney
Nicholson, Suellen
Perry, Faye
Paskin, Roger
Catton, Mike
Lester, Rosemary
MacKenzie, John
author_facet Williams, Stephanie
Richards, Jack
Faddy, Helen
Leydon, Jennie
Moran, Rodney
Nicholson, Suellen
Perry, Faye
Paskin, Roger
Catton, Mike
Lester, Rosemary
MacKenzie, John
author_sort Williams, Stephanie
building Curtin Institutional Repository
collection Online Access
description Objective: To assess evidence of recent and past exposure to Murray Valley encephalitis virus (MVEV) and West Nile clade Kunjin virus (KUNV) in residents of the Murray Valley, Victoria, during a period of demonstrated activity of both viruses in early 2011. Methods: A cross-sectional serosurvey using two convenience samples: stored serum specimens from a diagnostic laboratory in Mildura and blood donors from the Murray Valley region. Specimens were collected between April and July 2011. The main outcome measure was total antibody (IgM and IgG) reactivity against MVEV and KUNV measured using an enzyme immunoassay and defined as inhibiting binding of monoclonal antibodies by >50%, when compared to negative controls. Evidence of recent exposure was measured by the presence of MVEV and KUNV IgM detected by immunofluorescence. Results: Of 1,115 specimens, 24 (2.2%,95% CI 1.3-3.0%) were positive for MVEV total antibody, and all were negative for MVEV IgM. Of 1,116 specimens, 34 (3.1%, 95% CI 2.0-4.0%) were positive for KUNV total antibody, and 3 (0.27%) were KUNV IgM positive. Total antibody seroprevalence for both viruses was higher in residents born before 1974. Conclusions: Despite widespread MVEV and KUNV activity in early 2011, this study found that seroprevalence of antibodies to both viruses was low (<5%) and little evidence of recent exposure. Implications: Our findings suggest both viruses remain epizootic in the region and local residents remain potentially susceptible to future outbreaks.
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institution Curtin University Malaysia
institution_category Local University
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publishDate 2013
publisher Wiley-Blackwell Publishing Asia
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spelling curtin-20.500.11937-229242017-09-13T13:57:42Z Low seroprevalence of Murray Valley Encephalitis and Kunjin viruses in an opportunistic serosurvey, Victoria 2011 Williams, Stephanie Richards, Jack Faddy, Helen Leydon, Jennie Moran, Rodney Nicholson, Suellen Perry, Faye Paskin, Roger Catton, Mike Lester, Rosemary MacKenzie, John flavivirus Kunjin blood donor mosquito Murray Valley Encephalitis seroprevalence Objective: To assess evidence of recent and past exposure to Murray Valley encephalitis virus (MVEV) and West Nile clade Kunjin virus (KUNV) in residents of the Murray Valley, Victoria, during a period of demonstrated activity of both viruses in early 2011. Methods: A cross-sectional serosurvey using two convenience samples: stored serum specimens from a diagnostic laboratory in Mildura and blood donors from the Murray Valley region. Specimens were collected between April and July 2011. The main outcome measure was total antibody (IgM and IgG) reactivity against MVEV and KUNV measured using an enzyme immunoassay and defined as inhibiting binding of monoclonal antibodies by >50%, when compared to negative controls. Evidence of recent exposure was measured by the presence of MVEV and KUNV IgM detected by immunofluorescence. Results: Of 1,115 specimens, 24 (2.2%,95% CI 1.3-3.0%) were positive for MVEV total antibody, and all were negative for MVEV IgM. Of 1,116 specimens, 34 (3.1%, 95% CI 2.0-4.0%) were positive for KUNV total antibody, and 3 (0.27%) were KUNV IgM positive. Total antibody seroprevalence for both viruses was higher in residents born before 1974. Conclusions: Despite widespread MVEV and KUNV activity in early 2011, this study found that seroprevalence of antibodies to both viruses was low (<5%) and little evidence of recent exposure. Implications: Our findings suggest both viruses remain epizootic in the region and local residents remain potentially susceptible to future outbreaks. 2013 Journal Article http://hdl.handle.net/20.500.11937/22924 10.1111/1753-6405.12113 Wiley-Blackwell Publishing Asia unknown
spellingShingle flavivirus
Kunjin
blood donor
mosquito
Murray Valley Encephalitis
seroprevalence
Williams, Stephanie
Richards, Jack
Faddy, Helen
Leydon, Jennie
Moran, Rodney
Nicholson, Suellen
Perry, Faye
Paskin, Roger
Catton, Mike
Lester, Rosemary
MacKenzie, John
Low seroprevalence of Murray Valley Encephalitis and Kunjin viruses in an opportunistic serosurvey, Victoria 2011
title Low seroprevalence of Murray Valley Encephalitis and Kunjin viruses in an opportunistic serosurvey, Victoria 2011
title_full Low seroprevalence of Murray Valley Encephalitis and Kunjin viruses in an opportunistic serosurvey, Victoria 2011
title_fullStr Low seroprevalence of Murray Valley Encephalitis and Kunjin viruses in an opportunistic serosurvey, Victoria 2011
title_full_unstemmed Low seroprevalence of Murray Valley Encephalitis and Kunjin viruses in an opportunistic serosurvey, Victoria 2011
title_short Low seroprevalence of Murray Valley Encephalitis and Kunjin viruses in an opportunistic serosurvey, Victoria 2011
title_sort low seroprevalence of murray valley encephalitis and kunjin viruses in an opportunistic serosurvey, victoria 2011
topic flavivirus
Kunjin
blood donor
mosquito
Murray Valley Encephalitis
seroprevalence
url http://hdl.handle.net/20.500.11937/22924