IgG Responses to Pneumococcal and Haemophilus Influenzae Protein Antigens Are Not Impaired in Children with a History of Recurrent Acute Otitis Media

Background: Vaccines including conserved antigens from Streptococcus pneumoniae and nontypeable Haemophilus influenzae (NTHi) have the potential to reduce the burden of acute otitis media. Little is known about the antibody response to such antigens in young children with recurrent acute otitis medi...

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Main Authors: Wiertsema, S., Corscadden, K., Mowe, E., Zhang, Guicheng, Vijayasekaran, S., Coates, H., Mitchell, T., Thomas, W., Richmond, P., Kirkham, L.
Format: Journal Article
Published: 2012
Online Access:http://hdl.handle.net/20.500.11937/22817
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author Wiertsema, S.
Corscadden, K.
Mowe, E.
Zhang, Guicheng
Vijayasekaran, S.
Coates, H.
Mitchell, T.
Thomas, W.
Richmond, P.
Kirkham, L.
author_facet Wiertsema, S.
Corscadden, K.
Mowe, E.
Zhang, Guicheng
Vijayasekaran, S.
Coates, H.
Mitchell, T.
Thomas, W.
Richmond, P.
Kirkham, L.
author_sort Wiertsema, S.
building Curtin Institutional Repository
collection Online Access
description Background: Vaccines including conserved antigens from Streptococcus pneumoniae and nontypeable Haemophilus influenzae (NTHi) have the potential to reduce the burden of acute otitis media. Little is known about the antibody response to such antigens in young children with recurrent acute otitis media, however, it has been suggested antibody production may be impaired in these children. Methods: We measured serum IgG levels against 4 pneumococcal (PspA1, PspA 2, CbpA and Ply) and 3 NTHi (P4, P6 and PD) proteins in a cross-sectional study of 172 children under 3 years of age with a history of recurrent acute otitis media (median 7 episodes, requiring ventilation tube insertion) and 63 healthy age-matched controls, using a newly developed multiplex bead assay. Results: Children with a history of recurrent acute otitis media had significantly higher geometric mean serum IgG levels against NTHi proteins P4, P6 and PD compared with healthy controls, whereas there was no difference in antibody levels against pneumococcal protein antigens. In both children with and without a history of acute otitis media, antibody levels increased with age and were significantly higher in children colonised with S. pneumoniae or NTHi compared with children that were not colonised. Conclusions: Proteins from S. pneumoniae and NTHi induce serum IgG in children with a history of acute otitis media. The mechanisms in which proteins induce immunity and potential protection requires further investigation but the dogma of impaired antibody responses in children with recurrent acute otitis media should be reconsidered. © 2012 Wiertsema et al.
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spelling curtin-20.500.11937-228172017-09-13T13:58:23Z IgG Responses to Pneumococcal and Haemophilus Influenzae Protein Antigens Are Not Impaired in Children with a History of Recurrent Acute Otitis Media Wiertsema, S. Corscadden, K. Mowe, E. Zhang, Guicheng Vijayasekaran, S. Coates, H. Mitchell, T. Thomas, W. Richmond, P. Kirkham, L. Background: Vaccines including conserved antigens from Streptococcus pneumoniae and nontypeable Haemophilus influenzae (NTHi) have the potential to reduce the burden of acute otitis media. Little is known about the antibody response to such antigens in young children with recurrent acute otitis media, however, it has been suggested antibody production may be impaired in these children. Methods: We measured serum IgG levels against 4 pneumococcal (PspA1, PspA 2, CbpA and Ply) and 3 NTHi (P4, P6 and PD) proteins in a cross-sectional study of 172 children under 3 years of age with a history of recurrent acute otitis media (median 7 episodes, requiring ventilation tube insertion) and 63 healthy age-matched controls, using a newly developed multiplex bead assay. Results: Children with a history of recurrent acute otitis media had significantly higher geometric mean serum IgG levels against NTHi proteins P4, P6 and PD compared with healthy controls, whereas there was no difference in antibody levels against pneumococcal protein antigens. In both children with and without a history of acute otitis media, antibody levels increased with age and were significantly higher in children colonised with S. pneumoniae or NTHi compared with children that were not colonised. Conclusions: Proteins from S. pneumoniae and NTHi induce serum IgG in children with a history of acute otitis media. The mechanisms in which proteins induce immunity and potential protection requires further investigation but the dogma of impaired antibody responses in children with recurrent acute otitis media should be reconsidered. © 2012 Wiertsema et al. 2012 Journal Article http://hdl.handle.net/20.500.11937/22817 10.1371/journal.pone.0049061 unknown
spellingShingle Wiertsema, S.
Corscadden, K.
Mowe, E.
Zhang, Guicheng
Vijayasekaran, S.
Coates, H.
Mitchell, T.
Thomas, W.
Richmond, P.
Kirkham, L.
IgG Responses to Pneumococcal and Haemophilus Influenzae Protein Antigens Are Not Impaired in Children with a History of Recurrent Acute Otitis Media
title IgG Responses to Pneumococcal and Haemophilus Influenzae Protein Antigens Are Not Impaired in Children with a History of Recurrent Acute Otitis Media
title_full IgG Responses to Pneumococcal and Haemophilus Influenzae Protein Antigens Are Not Impaired in Children with a History of Recurrent Acute Otitis Media
title_fullStr IgG Responses to Pneumococcal and Haemophilus Influenzae Protein Antigens Are Not Impaired in Children with a History of Recurrent Acute Otitis Media
title_full_unstemmed IgG Responses to Pneumococcal and Haemophilus Influenzae Protein Antigens Are Not Impaired in Children with a History of Recurrent Acute Otitis Media
title_short IgG Responses to Pneumococcal and Haemophilus Influenzae Protein Antigens Are Not Impaired in Children with a History of Recurrent Acute Otitis Media
title_sort igg responses to pneumococcal and haemophilus influenzae protein antigens are not impaired in children with a history of recurrent acute otitis media
url http://hdl.handle.net/20.500.11937/22817