Formulation and Characterization of Drug-Loaded Microparticles Using Distillers Dried Grain Kafirin

Kafirin, a protein extracted from sorghum grain, has been formulated into microparticles and proposed for use as a delivery system owing to the resistance of kafirin to upper gastrointestinal digestion. However, extracting kafirin from sorghum distillers dried grains with solubles (DDGS) may be more...

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Main Authors: Lau, E., Johnson, Stuart, Stanley, R., Mereddy, R., Mikkelsen, D., Halley, P., Steadman, K.
Format: Journal Article
Published: A A C C International 2015
Online Access:http://hdl.handle.net/20.500.11937/22668
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author Lau, E.
Johnson, Stuart
Stanley, R.
Mereddy, R.
Mikkelsen, D.
Halley, P.
Steadman, K.
author_facet Lau, E.
Johnson, Stuart
Stanley, R.
Mereddy, R.
Mikkelsen, D.
Halley, P.
Steadman, K.
author_sort Lau, E.
building Curtin Institutional Repository
collection Online Access
description Kafirin, a protein extracted from sorghum grain, has been formulated into microparticles and proposed for use as a delivery system owing to the resistance of kafirin to upper gastrointestinal digestion. However, extracting kafirin from sorghum distillers dried grains with solubles (DDGS) may be more efficient, because the carbohydrate component has been removed by fermentation. This study investigated the properties and use of kafirin extracted from DDGS to formulate microparticles. Prednisolone, an anti-inflammatory drug that could benefit from a delayed and targeted delivery system to the colon, was loaded into DDGS kafirin microparticles by phase separation with sodium chloride. Scanning electron micrographs revealed that the empty and prednisolone-loaded microparticles were round in shape and varied in size. Surface binding studies indicated prednisolone was loaded within the microparticles rather than being solely bound on the surface. These findings demonstrate DDGS kafirin can be formulated into microparticles and loaded with medication. Future studies could investigate the potential applications of DDGS kafirin microparticles as an orally administered targeted drug-delivery system.
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publishDate 2015
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spelling curtin-20.500.11937-226682017-09-13T13:57:44Z Formulation and Characterization of Drug-Loaded Microparticles Using Distillers Dried Grain Kafirin Lau, E. Johnson, Stuart Stanley, R. Mereddy, R. Mikkelsen, D. Halley, P. Steadman, K. Kafirin, a protein extracted from sorghum grain, has been formulated into microparticles and proposed for use as a delivery system owing to the resistance of kafirin to upper gastrointestinal digestion. However, extracting kafirin from sorghum distillers dried grains with solubles (DDGS) may be more efficient, because the carbohydrate component has been removed by fermentation. This study investigated the properties and use of kafirin extracted from DDGS to formulate microparticles. Prednisolone, an anti-inflammatory drug that could benefit from a delayed and targeted delivery system to the colon, was loaded into DDGS kafirin microparticles by phase separation with sodium chloride. Scanning electron micrographs revealed that the empty and prednisolone-loaded microparticles were round in shape and varied in size. Surface binding studies indicated prednisolone was loaded within the microparticles rather than being solely bound on the surface. These findings demonstrate DDGS kafirin can be formulated into microparticles and loaded with medication. Future studies could investigate the potential applications of DDGS kafirin microparticles as an orally administered targeted drug-delivery system. 2015 Journal Article http://hdl.handle.net/20.500.11937/22668 10.1094/CCHEM-05-14-0096-R A A C C International restricted
spellingShingle Lau, E.
Johnson, Stuart
Stanley, R.
Mereddy, R.
Mikkelsen, D.
Halley, P.
Steadman, K.
Formulation and Characterization of Drug-Loaded Microparticles Using Distillers Dried Grain Kafirin
title Formulation and Characterization of Drug-Loaded Microparticles Using Distillers Dried Grain Kafirin
title_full Formulation and Characterization of Drug-Loaded Microparticles Using Distillers Dried Grain Kafirin
title_fullStr Formulation and Characterization of Drug-Loaded Microparticles Using Distillers Dried Grain Kafirin
title_full_unstemmed Formulation and Characterization of Drug-Loaded Microparticles Using Distillers Dried Grain Kafirin
title_short Formulation and Characterization of Drug-Loaded Microparticles Using Distillers Dried Grain Kafirin
title_sort formulation and characterization of drug-loaded microparticles using distillers dried grain kafirin
url http://hdl.handle.net/20.500.11937/22668