Isolation and characterization of polymorphic microsatellite DNA markers for Banksia candolleana (Proteaceae)

We developed 11 polymorphic microsatellite DNA markers for the Australian native shrub Banksia candolleana (Proteaceae). For 39 sampled individuals, the number of alleles per locus varied from four to eleven, while observed and expected heterozygosities ranged from 0.233 to 0.872, and 0.305 to 0.856...

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Main Authors: Merwin, L., He, Tianhua, Krauss, S.
Format: Journal Article
Published: Springer 2010
Online Access:http://hdl.handle.net/20.500.11937/22624
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author Merwin, L.
He, Tianhua
Krauss, S.
author_facet Merwin, L.
He, Tianhua
Krauss, S.
author_sort Merwin, L.
building Curtin Institutional Repository
collection Online Access
description We developed 11 polymorphic microsatellite DNA markers for the Australian native shrub Banksia candolleana (Proteaceae). For 39 sampled individuals, the number of alleles per locus varied from four to eleven, while observed and expected heterozygosities ranged from 0.233 to 0.872, and 0.305 to 0.856, respectively. Three loci (BC-B1, BC-B2, and BC-A6) showed significant deviation from Hardy-Weinberg equilibrium (P<0.05), and null alleles may be present at BC-B1 and BC-A6. These loci were suitably polymorphic for future studies assessing metapopulation dispersal dynamics. © Springer Science+Business Media B.V. 2010.
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institution Curtin University Malaysia
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spelling curtin-20.500.11937-226242017-09-13T13:57:04Z Isolation and characterization of polymorphic microsatellite DNA markers for Banksia candolleana (Proteaceae) Merwin, L. He, Tianhua Krauss, S. We developed 11 polymorphic microsatellite DNA markers for the Australian native shrub Banksia candolleana (Proteaceae). For 39 sampled individuals, the number of alleles per locus varied from four to eleven, while observed and expected heterozygosities ranged from 0.233 to 0.872, and 0.305 to 0.856, respectively. Three loci (BC-B1, BC-B2, and BC-A6) showed significant deviation from Hardy-Weinberg equilibrium (P<0.05), and null alleles may be present at BC-B1 and BC-A6. These loci were suitably polymorphic for future studies assessing metapopulation dispersal dynamics. © Springer Science+Business Media B.V. 2010. 2010 Journal Article http://hdl.handle.net/20.500.11937/22624 10.1007/s12686-010-9242-1 Springer restricted
spellingShingle Merwin, L.
He, Tianhua
Krauss, S.
Isolation and characterization of polymorphic microsatellite DNA markers for Banksia candolleana (Proteaceae)
title Isolation and characterization of polymorphic microsatellite DNA markers for Banksia candolleana (Proteaceae)
title_full Isolation and characterization of polymorphic microsatellite DNA markers for Banksia candolleana (Proteaceae)
title_fullStr Isolation and characterization of polymorphic microsatellite DNA markers for Banksia candolleana (Proteaceae)
title_full_unstemmed Isolation and characterization of polymorphic microsatellite DNA markers for Banksia candolleana (Proteaceae)
title_short Isolation and characterization of polymorphic microsatellite DNA markers for Banksia candolleana (Proteaceae)
title_sort isolation and characterization of polymorphic microsatellite dna markers for banksia candolleana (proteaceae)
url http://hdl.handle.net/20.500.11937/22624