The Rapid Analysis of Heroin Drug Seizures Using Micellar Electrokinetic Chromatography with Short-End Injection

A simple and rapid method for the analysis of heroin seizures by micellar electrokinetic chromatography with short-end injection is described. Separations were performed using an uncoated fused silica capillary, 50 cm×50µm I.D.×360µm O.D. with an effective separation length of 8 cm. The system was r...

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Main Authors: Anastos, N., Lewis, Simon, Barnett, N., Pearson, J., Kirkbride, K.
Format: Journal Article
Published: Blackwell Publishing 2005
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/22540
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author Anastos, N.
Lewis, Simon
Barnett, N.
Pearson, J.
Kirkbride, K.
author_facet Anastos, N.
Lewis, Simon
Barnett, N.
Pearson, J.
Kirkbride, K.
author_sort Anastos, N.
building Curtin Institutional Repository
collection Online Access
description A simple and rapid method for the analysis of heroin seizures by micellar electrokinetic chromatography with short-end injection is described. Separations were performed using an uncoated fused silica capillary, 50 cm×50µm I.D.×360µm O.D. with an effective separation length of 8 cm. The system was run at 25?C with an applied negative voltage of –25 kilovolts. Injection of each sample was for 2 s at –50 mbar. UV detection was employed with the wavelength set at 210 nm. The background electrolyte consisted of 85:15 water:acetonitrile, v/v) containing final concentrations of 25mMSDS and 15mMsodium borate, pH 9.5. Samples and standards were prepared in 0.1% v/v acetic acid and diluted in the run buffer containing 1 mg/ml of N,N-dimethyl-5-methoxytryptamine as an internal standard. Under these conditions a text mixture containing caffeine, paracetamol, morphine, codeine, heroin, and acetylcodeine was resolved within 1.5 min. The method was used to determine the concentration of heroin in heroin seizure samples, and the results were in good agreement with those obtained by a validated gas chromatographic method.
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publishDate 2005
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spelling curtin-20.500.11937-225402017-02-28T01:36:46Z The Rapid Analysis of Heroin Drug Seizures Using Micellar Electrokinetic Chromatography with Short-End Injection Anastos, N. Lewis, Simon Barnett, N. Pearson, J. Kirkbride, K. micellar electrokinetic chromatography short-end injection forensic science heroin seizures A simple and rapid method for the analysis of heroin seizures by micellar electrokinetic chromatography with short-end injection is described. Separations were performed using an uncoated fused silica capillary, 50 cm×50µm I.D.×360µm O.D. with an effective separation length of 8 cm. The system was run at 25?C with an applied negative voltage of –25 kilovolts. Injection of each sample was for 2 s at –50 mbar. UV detection was employed with the wavelength set at 210 nm. The background electrolyte consisted of 85:15 water:acetonitrile, v/v) containing final concentrations of 25mMSDS and 15mMsodium borate, pH 9.5. Samples and standards were prepared in 0.1% v/v acetic acid and diluted in the run buffer containing 1 mg/ml of N,N-dimethyl-5-methoxytryptamine as an internal standard. Under these conditions a text mixture containing caffeine, paracetamol, morphine, codeine, heroin, and acetylcodeine was resolved within 1.5 min. The method was used to determine the concentration of heroin in heroin seizure samples, and the results were in good agreement with those obtained by a validated gas chromatographic method. 2005 Journal Article http://hdl.handle.net/20.500.11937/22540 Blackwell Publishing restricted
spellingShingle micellar electrokinetic chromatography
short-end injection
forensic science
heroin seizures
Anastos, N.
Lewis, Simon
Barnett, N.
Pearson, J.
Kirkbride, K.
The Rapid Analysis of Heroin Drug Seizures Using Micellar Electrokinetic Chromatography with Short-End Injection
title The Rapid Analysis of Heroin Drug Seizures Using Micellar Electrokinetic Chromatography with Short-End Injection
title_full The Rapid Analysis of Heroin Drug Seizures Using Micellar Electrokinetic Chromatography with Short-End Injection
title_fullStr The Rapid Analysis of Heroin Drug Seizures Using Micellar Electrokinetic Chromatography with Short-End Injection
title_full_unstemmed The Rapid Analysis of Heroin Drug Seizures Using Micellar Electrokinetic Chromatography with Short-End Injection
title_short The Rapid Analysis of Heroin Drug Seizures Using Micellar Electrokinetic Chromatography with Short-End Injection
title_sort rapid analysis of heroin drug seizures using micellar electrokinetic chromatography with short-end injection
topic micellar electrokinetic chromatography
short-end injection
forensic science
heroin seizures
url http://hdl.handle.net/20.500.11937/22540