The glycoprotein fibulin-3 regulates morphology and motility of olfactory ensheathing cells in vitro
The primary olfactory pathway in adult mammals has retained a remarkable potential for self-repair. A specialized glial cell within the olfactory nerve, called olfactory ensheathing cell (OEC), and their associated extracellular matrix are thought to play an important role during regenerative events...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
| Published: |
2009
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| Online Access: | http://hdl.handle.net/20.500.11937/22478 |
| _version_ | 1848750881204338688 |
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| author | Vukovic, J. Ruitenberg, M. Roet, K. Franssen, E. Arulpragasam, Ajanthy Sasaki, T. Verhaagen, J. Harvey, A. Busfield, S. Plant, G. |
| author_facet | Vukovic, J. Ruitenberg, M. Roet, K. Franssen, E. Arulpragasam, Ajanthy Sasaki, T. Verhaagen, J. Harvey, A. Busfield, S. Plant, G. |
| author_sort | Vukovic, J. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The primary olfactory pathway in adult mammals has retained a remarkable potential for self-repair. A specialized glial cell within the olfactory nerve, called olfactory ensheathing cell (OEC), and their associated extracellular matrix are thought to play an important role during regenerative events in this system. To gain insight into novel molecules that could mediate the OEC-supported growth of axons within the olfactory nerve, gene expression profiling experiments were conducted which revealed high expression of the glycoprotein fibulin-3 in OECs. This observation was confirmed with quantitative PCR. In vivo, the distribution of all members of the fibulin family, fibulin-3 included, was localized to the lamina propria underneath the olfactory epithelium, in close association within olfactory nerve bundles. To manipulate fibulin-3 gene expression in cultured OECs, lentiviral vector constructs were designed to either transgenically express or knock-down fibulin-3. Experimental data showed that increased levels of fibulin-3 induced profound morphological changes in cultured OECs, impeded with their migratory abilities and also suppressed OEC-mediated neurite outgrowth. Knock-down of fibulin-3 levels resulted in reduced OEC proliferation. In conclusion, the data provide novel insights into a putative role for fibulin-3 in the regulation of cell migration and neurite outgrowth within the primary olfactory pathway. © 2008 Wiley-Liss, Inc. |
| first_indexed | 2025-11-14T07:43:52Z |
| format | Journal Article |
| id | curtin-20.500.11937-22478 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:43:52Z |
| publishDate | 2009 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-224782017-09-13T13:52:23Z The glycoprotein fibulin-3 regulates morphology and motility of olfactory ensheathing cells in vitro Vukovic, J. Ruitenberg, M. Roet, K. Franssen, E. Arulpragasam, Ajanthy Sasaki, T. Verhaagen, J. Harvey, A. Busfield, S. Plant, G. The primary olfactory pathway in adult mammals has retained a remarkable potential for self-repair. A specialized glial cell within the olfactory nerve, called olfactory ensheathing cell (OEC), and their associated extracellular matrix are thought to play an important role during regenerative events in this system. To gain insight into novel molecules that could mediate the OEC-supported growth of axons within the olfactory nerve, gene expression profiling experiments were conducted which revealed high expression of the glycoprotein fibulin-3 in OECs. This observation was confirmed with quantitative PCR. In vivo, the distribution of all members of the fibulin family, fibulin-3 included, was localized to the lamina propria underneath the olfactory epithelium, in close association within olfactory nerve bundles. To manipulate fibulin-3 gene expression in cultured OECs, lentiviral vector constructs were designed to either transgenically express or knock-down fibulin-3. Experimental data showed that increased levels of fibulin-3 induced profound morphological changes in cultured OECs, impeded with their migratory abilities and also suppressed OEC-mediated neurite outgrowth. Knock-down of fibulin-3 levels resulted in reduced OEC proliferation. In conclusion, the data provide novel insights into a putative role for fibulin-3 in the regulation of cell migration and neurite outgrowth within the primary olfactory pathway. © 2008 Wiley-Liss, Inc. 2009 Journal Article http://hdl.handle.net/20.500.11937/22478 10.1002/glia.20771 restricted |
| spellingShingle | Vukovic, J. Ruitenberg, M. Roet, K. Franssen, E. Arulpragasam, Ajanthy Sasaki, T. Verhaagen, J. Harvey, A. Busfield, S. Plant, G. The glycoprotein fibulin-3 regulates morphology and motility of olfactory ensheathing cells in vitro |
| title | The glycoprotein fibulin-3 regulates morphology and motility of olfactory ensheathing cells in vitro |
| title_full | The glycoprotein fibulin-3 regulates morphology and motility of olfactory ensheathing cells in vitro |
| title_fullStr | The glycoprotein fibulin-3 regulates morphology and motility of olfactory ensheathing cells in vitro |
| title_full_unstemmed | The glycoprotein fibulin-3 regulates morphology and motility of olfactory ensheathing cells in vitro |
| title_short | The glycoprotein fibulin-3 regulates morphology and motility of olfactory ensheathing cells in vitro |
| title_sort | glycoprotein fibulin-3 regulates morphology and motility of olfactory ensheathing cells in vitro |
| url | http://hdl.handle.net/20.500.11937/22478 |