Decay of salmonella enterica, escherichia coli and bacteriophage ms2 on the phyllosphere and stored grains of wheat (triticum aestivum)

Cereal crops grown in the biosolids-amended soil can potentially become contaminated with pathogenic micro-organisms during growth and at the time of harvesting. There is small but unquantified potential risk of transfer of enteric pathogens to humans and animals from contaminated plants and grains....

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Main Authors: Schwarz, Karen, Sidhu, J., Pritchard, Deborah, Li, Y., Toze, S.
Format: Journal Article
Published: Blackwell Scientific Publishers 2014
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/21289
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author Schwarz, Karen
Sidhu, J.
Pritchard, Deborah
Li, Y.
Toze, S.
author_facet Schwarz, Karen
Sidhu, J.
Pritchard, Deborah
Li, Y.
Toze, S.
author_sort Schwarz, Karen
building Curtin Institutional Repository
collection Online Access
description Cereal crops grown in the biosolids-amended soil can potentially become contaminated with pathogenic micro-organisms during growth and at the time of harvesting. There is small but unquantified potential risk of transfer of enteric pathogens to humans and animals from contaminated plants and grains. This study examined decay of Escherichia coli, Salmonella enterica serovar Typhimurium and bacteriophage MS2 on the wheat phyllosphere and on stored grains. This was done to assess the health implications of cereal crops contaminated from land application of biosolids. E. coli, S. enterica and MS2 were inoculated onto the leaves, spikelets and grains of wheat. The change in the numbers of inoculated micro-organisms was determined over time to calculate the respective 90% reduction time (T90) in each of these environments. A rapid inactivation (T90 <1–3 days) of E. coli and S. enterica and MS2 from the plant phyllosphere was observed, particularly from the spikelets. The decay rates were influenced by micro-organism type and location on the plant phyllosphere. Decay times on the stored grains were longer (T90 9–71 days), with some observed influence of grain variety on pathogen decay times.
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institution Curtin University Malaysia
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publishDate 2014
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spelling curtin-20.500.11937-212892017-09-13T13:53:06Z Decay of salmonella enterica, escherichia coli and bacteriophage ms2 on the phyllosphere and stored grains of wheat (triticum aestivum) Schwarz, Karen Sidhu, J. Pritchard, Deborah Li, Y. Toze, S. wheat phyllosphere adenovirus Salmonella enterica biosolids pathogens Cereal crops grown in the biosolids-amended soil can potentially become contaminated with pathogenic micro-organisms during growth and at the time of harvesting. There is small but unquantified potential risk of transfer of enteric pathogens to humans and animals from contaminated plants and grains. This study examined decay of Escherichia coli, Salmonella enterica serovar Typhimurium and bacteriophage MS2 on the wheat phyllosphere and on stored grains. This was done to assess the health implications of cereal crops contaminated from land application of biosolids. E. coli, S. enterica and MS2 were inoculated onto the leaves, spikelets and grains of wheat. The change in the numbers of inoculated micro-organisms was determined over time to calculate the respective 90% reduction time (T90) in each of these environments. A rapid inactivation (T90 <1–3 days) of E. coli and S. enterica and MS2 from the plant phyllosphere was observed, particularly from the spikelets. The decay rates were influenced by micro-organism type and location on the plant phyllosphere. Decay times on the stored grains were longer (T90 9–71 days), with some observed influence of grain variety on pathogen decay times. 2014 Journal Article http://hdl.handle.net/20.500.11937/21289 10.1111/lam.12149 Blackwell Scientific Publishers unknown
spellingShingle wheat
phyllosphere
adenovirus
Salmonella enterica
biosolids
pathogens
Schwarz, Karen
Sidhu, J.
Pritchard, Deborah
Li, Y.
Toze, S.
Decay of salmonella enterica, escherichia coli and bacteriophage ms2 on the phyllosphere and stored grains of wheat (triticum aestivum)
title Decay of salmonella enterica, escherichia coli and bacteriophage ms2 on the phyllosphere and stored grains of wheat (triticum aestivum)
title_full Decay of salmonella enterica, escherichia coli and bacteriophage ms2 on the phyllosphere and stored grains of wheat (triticum aestivum)
title_fullStr Decay of salmonella enterica, escherichia coli and bacteriophage ms2 on the phyllosphere and stored grains of wheat (triticum aestivum)
title_full_unstemmed Decay of salmonella enterica, escherichia coli and bacteriophage ms2 on the phyllosphere and stored grains of wheat (triticum aestivum)
title_short Decay of salmonella enterica, escherichia coli and bacteriophage ms2 on the phyllosphere and stored grains of wheat (triticum aestivum)
title_sort decay of salmonella enterica, escherichia coli and bacteriophage ms2 on the phyllosphere and stored grains of wheat (triticum aestivum)
topic wheat
phyllosphere
adenovirus
Salmonella enterica
biosolids
pathogens
url http://hdl.handle.net/20.500.11937/21289