Mannitol is required for asexual sporulation in the wheat pathogen Stagonospora nodorum (glume blotch)
The physiological role of the mannitol cycle in the wheat pathogen Stagonospora nodorum (glume blotch) has been investigated by reverse genetics and metabolite profiling. A putative mannitol 2-dehydrogenase gene (Mdh1) was cloned by degenerate PCR and disrupted. The resulting mutated mdh1 strains la...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal Article |
| Published: |
Portland Press Ltd.
2006
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/21235 |
| _version_ | 1848750532955471872 |
|---|---|
| author | Solomon, P. Waters, O. Jorgens, C. Lowe, R. Rechberger, J. Trengove, R. Oliver, Richard |
| author_facet | Solomon, P. Waters, O. Jorgens, C. Lowe, R. Rechberger, J. Trengove, R. Oliver, Richard |
| author_sort | Solomon, P. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The physiological role of the mannitol cycle in the wheat pathogen Stagonospora nodorum (glume blotch) has been investigated by reverse genetics and metabolite profiling. A putative mannitol 2-dehydrogenase gene (Mdh1) was cloned by degenerate PCR and disrupted. The resulting mutated mdh1 strains lacked all detectable NADPH-dependent mannitol dehydrogenase activity. The mdh1 strains were unaffected for mannitol production but, surprisingly, were still able to utilize mannitol as a sole carbon source, suggesting a hitherto unknown mechanism for mannitol catabolism. The mutant strains were not compromised in their ability to cause disease or sporulate. To further our understanding of mannitol metabolism, a previously developed mannitol-1-phosphate dehydrogenase (gene mpd1) disruption construct [Solomon, Tan and Oliver (2005) Mol. Plant-Microbe Interact. 18, 110-115] was introduced into the mutated mdh1 background, resulting in a strain lacking both enzyme activities. The mpd1 mdh1 strains were unable to grow on mannitol and produced only trace levels of mannitol. The double-mutant strains were unable to sporulate in vitro when grown on minimal medium for extended periods. Deficiency in sporulation was correlated with the depletion of intracellular mannitol pools. Significantly sporulation could be restored with the addition of mannitol. Pathogenicity of the double mutant was not compromised, although, like the previously characterized mpd1 mutants, the strains were unable to sporulate in planta. These findings not only question the currently hypothesized pathways of mannitol metabolism, but also identify for the first time that mannitol is required for sporulation of a filamentous fungus. |
| first_indexed | 2025-11-14T07:38:20Z |
| format | Journal Article |
| id | curtin-20.500.11937-21235 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:38:20Z |
| publishDate | 2006 |
| publisher | Portland Press Ltd. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-212352019-05-21T07:41:11Z Mannitol is required for asexual sporulation in the wheat pathogen Stagonospora nodorum (glume blotch) Solomon, P. Waters, O. Jorgens, C. Lowe, R. Rechberger, J. Trengove, R. Oliver, Richard AGARICUS-BISPORUS FUNGI NADPH PROTEIN DATABASE SYN FULVIA-FULVA DEHYDROGENASE METABOLISM CYCLE ASPERGILLUS-NIDULANS The physiological role of the mannitol cycle in the wheat pathogen Stagonospora nodorum (glume blotch) has been investigated by reverse genetics and metabolite profiling. A putative mannitol 2-dehydrogenase gene (Mdh1) was cloned by degenerate PCR and disrupted. The resulting mutated mdh1 strains lacked all detectable NADPH-dependent mannitol dehydrogenase activity. The mdh1 strains were unaffected for mannitol production but, surprisingly, were still able to utilize mannitol as a sole carbon source, suggesting a hitherto unknown mechanism for mannitol catabolism. The mutant strains were not compromised in their ability to cause disease or sporulate. To further our understanding of mannitol metabolism, a previously developed mannitol-1-phosphate dehydrogenase (gene mpd1) disruption construct [Solomon, Tan and Oliver (2005) Mol. Plant-Microbe Interact. 18, 110-115] was introduced into the mutated mdh1 background, resulting in a strain lacking both enzyme activities. The mpd1 mdh1 strains were unable to grow on mannitol and produced only trace levels of mannitol. The double-mutant strains were unable to sporulate in vitro when grown on minimal medium for extended periods. Deficiency in sporulation was correlated with the depletion of intracellular mannitol pools. Significantly sporulation could be restored with the addition of mannitol. Pathogenicity of the double mutant was not compromised, although, like the previously characterized mpd1 mutants, the strains were unable to sporulate in planta. These findings not only question the currently hypothesized pathways of mannitol metabolism, but also identify for the first time that mannitol is required for sporulation of a filamentous fungus. 2006 Journal Article http://hdl.handle.net/20.500.11937/21235 10.1042/BJ20060891 Portland Press Ltd. restricted |
| spellingShingle | AGARICUS-BISPORUS FUNGI NADPH PROTEIN DATABASE SYN FULVIA-FULVA DEHYDROGENASE METABOLISM CYCLE ASPERGILLUS-NIDULANS Solomon, P. Waters, O. Jorgens, C. Lowe, R. Rechberger, J. Trengove, R. Oliver, Richard Mannitol is required for asexual sporulation in the wheat pathogen Stagonospora nodorum (glume blotch) |
| title | Mannitol is required for asexual sporulation in the wheat pathogen Stagonospora nodorum (glume blotch) |
| title_full | Mannitol is required for asexual sporulation in the wheat pathogen Stagonospora nodorum (glume blotch) |
| title_fullStr | Mannitol is required for asexual sporulation in the wheat pathogen Stagonospora nodorum (glume blotch) |
| title_full_unstemmed | Mannitol is required for asexual sporulation in the wheat pathogen Stagonospora nodorum (glume blotch) |
| title_short | Mannitol is required for asexual sporulation in the wheat pathogen Stagonospora nodorum (glume blotch) |
| title_sort | mannitol is required for asexual sporulation in the wheat pathogen stagonospora nodorum (glume blotch) |
| topic | AGARICUS-BISPORUS FUNGI NADPH PROTEIN DATABASE SYN FULVIA-FULVA DEHYDROGENASE METABOLISM CYCLE ASPERGILLUS-NIDULANS |
| url | http://hdl.handle.net/20.500.11937/21235 |