Generation of a ToxA knockout strain of the wheat tan spot pathogen Pyrenophora tritici-repentis
The necrotrophic fungal pathogen Pyrenophora tritici-repentis causes tan spot, a major disease of wheat throughout the world. The proteinaceous effector ToxA is responsible for foliar necrosis on ToxA sensitive wheat genotypes. The single copy ToxA gene was deleted from a wild-type race 1 P. tritici...
| Main Authors: | , , |
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| Format: | Journal Article |
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Wiley-Blackwell Publishing Ltd.
2014
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| Online Access: | http://hdl.handle.net/20.500.11937/21045 |
| _version_ | 1848750480895770624 |
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| author | Moffat, Caroline See, Pao Theen Oliver, Richard |
| author_facet | Moffat, Caroline See, Pao Theen Oliver, Richard |
| author_sort | Moffat, Caroline |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The necrotrophic fungal pathogen Pyrenophora tritici-repentis causes tan spot, a major disease of wheat throughout the world. The proteinaceous effector ToxA is responsible for foliar necrosis on ToxA sensitive wheat genotypes. The single copy ToxA gene was deleted from a wild-type race 1 P. tritici-repentis isolate via homologous recombination of a knockout construct. Expression of the ToxA transcript was found to be absent in transformants (toxa), as was ToxA protein production in fungal culture filtrates. Plant bioassays were conducted to test transformant pathogenicity. The toxa strains were unable to induce necrosis on ToxA sensitivity wheat genotypes. To our knowledge, this is the first demonstration of a targeted gene knockout in P. tritici-repentis. The ability to undertake gene deletions will facilitate the characterisation of other pathogenicity effectors of this economically significant necrotroph. |
| first_indexed | 2025-11-14T07:37:31Z |
| format | Journal Article |
| id | curtin-20.500.11937-21045 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:37:31Z |
| publishDate | 2014 |
| publisher | Wiley-Blackwell Publishing Ltd. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-210452019-03-28T05:28:54Z Generation of a ToxA knockout strain of the wheat tan spot pathogen Pyrenophora tritici-repentis Moffat, Caroline See, Pao Theen Oliver, Richard Transformation Yellow leaf spot Yellow spot Homologous recombination The necrotrophic fungal pathogen Pyrenophora tritici-repentis causes tan spot, a major disease of wheat throughout the world. The proteinaceous effector ToxA is responsible for foliar necrosis on ToxA sensitive wheat genotypes. The single copy ToxA gene was deleted from a wild-type race 1 P. tritici-repentis isolate via homologous recombination of a knockout construct. Expression of the ToxA transcript was found to be absent in transformants (toxa), as was ToxA protein production in fungal culture filtrates. Plant bioassays were conducted to test transformant pathogenicity. The toxa strains were unable to induce necrosis on ToxA sensitivity wheat genotypes. To our knowledge, this is the first demonstration of a targeted gene knockout in P. tritici-repentis. The ability to undertake gene deletions will facilitate the characterisation of other pathogenicity effectors of this economically significant necrotroph. 2014 Journal Article http://hdl.handle.net/20.500.11937/21045 10.1111/mpp.12154 Wiley-Blackwell Publishing Ltd. fulltext |
| spellingShingle | Transformation Yellow leaf spot Yellow spot Homologous recombination Moffat, Caroline See, Pao Theen Oliver, Richard Generation of a ToxA knockout strain of the wheat tan spot pathogen Pyrenophora tritici-repentis |
| title | Generation of a ToxA knockout strain of the wheat tan spot pathogen Pyrenophora tritici-repentis |
| title_full | Generation of a ToxA knockout strain of the wheat tan spot pathogen Pyrenophora tritici-repentis |
| title_fullStr | Generation of a ToxA knockout strain of the wheat tan spot pathogen Pyrenophora tritici-repentis |
| title_full_unstemmed | Generation of a ToxA knockout strain of the wheat tan spot pathogen Pyrenophora tritici-repentis |
| title_short | Generation of a ToxA knockout strain of the wheat tan spot pathogen Pyrenophora tritici-repentis |
| title_sort | generation of a toxa knockout strain of the wheat tan spot pathogen pyrenophora tritici-repentis |
| topic | Transformation Yellow leaf spot Yellow spot Homologous recombination |
| url | http://hdl.handle.net/20.500.11937/21045 |