Development of microsatellite markers for two Australian Persoonia (Proteaceae) species using two different techniques.
UNLABELLED: • PREMISE OF THE STUDY: Microsatellite markers were developed and cross-species transferability assessed for two Persoonia species to evaluate genetic diversity and population genetic structure of these broadly distributed southwest Australian tree species. • METHODS AND RESULTS: Microsa...
| Main Authors: | , , , |
|---|---|
| Format: | Journal Article |
| Published: |
2013
|
| Online Access: | http://hdl.handle.net/20.500.11937/21030 |
| _version_ | 1848750476397379584 |
|---|---|
| author | Stingemore, J. Nevill, Paul Gardner, M. Krauss, S. |
| author_facet | Stingemore, J. Nevill, Paul Gardner, M. Krauss, S. |
| author_sort | Stingemore, J. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | UNLABELLED: • PREMISE OF THE STUDY: Microsatellite markers were developed and cross-species transferability assessed for two Persoonia species to evaluate genetic diversity and population genetic structure of these broadly distributed southwest Australian tree species. • METHODS AND RESULTS: Microsatellite-enriched libraries and 454 GS-FLX shotgun sequencing were used to identity nine microsatellite loci for P. elliptica (one 454; eight cloning) and six for P. longifolia (three 454; three cloning). These loci were screened for variation in individuals from populations in southwestern Australia. In P. elliptica, observed and expected heterozygosities ranged from 0.46 to 0.93 and 0.42 to 0.88, respectively. For P. longifolia, observed and expected heterozygosities ranged from 0.04 to 0.88 and 0.04 to 0.84, respectively. • CONCLUSIONS: The microsatellites identified in this study will enable the examination of population and spatial structuring of genetic diversity in P. elliptica and P. longifolia, two priority species for mine site restoration in southwestern Australia. |
| first_indexed | 2025-11-14T07:37:26Z |
| format | Journal Article |
| id | curtin-20.500.11937-21030 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:37:26Z |
| publishDate | 2013 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-210302017-09-13T13:46:01Z Development of microsatellite markers for two Australian Persoonia (Proteaceae) species using two different techniques. Stingemore, J. Nevill, Paul Gardner, M. Krauss, S. UNLABELLED: • PREMISE OF THE STUDY: Microsatellite markers were developed and cross-species transferability assessed for two Persoonia species to evaluate genetic diversity and population genetic structure of these broadly distributed southwest Australian tree species. • METHODS AND RESULTS: Microsatellite-enriched libraries and 454 GS-FLX shotgun sequencing were used to identity nine microsatellite loci for P. elliptica (one 454; eight cloning) and six for P. longifolia (three 454; three cloning). These loci were screened for variation in individuals from populations in southwestern Australia. In P. elliptica, observed and expected heterozygosities ranged from 0.46 to 0.93 and 0.42 to 0.88, respectively. For P. longifolia, observed and expected heterozygosities ranged from 0.04 to 0.88 and 0.04 to 0.84, respectively. • CONCLUSIONS: The microsatellites identified in this study will enable the examination of population and spatial structuring of genetic diversity in P. elliptica and P. longifolia, two priority species for mine site restoration in southwestern Australia. 2013 Journal Article http://hdl.handle.net/20.500.11937/21030 10.3732/apps.1300023 unknown |
| spellingShingle | Stingemore, J. Nevill, Paul Gardner, M. Krauss, S. Development of microsatellite markers for two Australian Persoonia (Proteaceae) species using two different techniques. |
| title | Development of microsatellite markers for two Australian Persoonia (Proteaceae) species using two different techniques. |
| title_full | Development of microsatellite markers for two Australian Persoonia (Proteaceae) species using two different techniques. |
| title_fullStr | Development of microsatellite markers for two Australian Persoonia (Proteaceae) species using two different techniques. |
| title_full_unstemmed | Development of microsatellite markers for two Australian Persoonia (Proteaceae) species using two different techniques. |
| title_short | Development of microsatellite markers for two Australian Persoonia (Proteaceae) species using two different techniques. |
| title_sort | development of microsatellite markers for two australian persoonia (proteaceae) species using two different techniques. |
| url | http://hdl.handle.net/20.500.11937/21030 |