Kafirin Adsorption on Ion-Exchange resins: Isotherm and Kinetic Studies
Today kafirin is found to be useful in designing delayed delivery systems and coatings of pharmaceuticals and nutraceuticals where its purity is important and this can be obtained by adsorptive chromatography. This study is the first scientific insight into the isotherm and kinetic studies of kafiri...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
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Elsevier BV
2014
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| Online Access: | http://hdl.handle.net/20.500.11937/21014 |
| _version_ | 1848750471464878080 |
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| author | Kumar, P. Lau, P. Kale, S. Johnson, Stuart Pareek, Vishnu Utikar, Ranjeet Lali, A. |
| author_facet | Kumar, P. Lau, P. Kale, S. Johnson, Stuart Pareek, Vishnu Utikar, Ranjeet Lali, A. |
| author_sort | Kumar, P. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Today kafirin is found to be useful in designing delayed delivery systems and coatings of pharmaceuticals and nutraceuticals where its purity is important and this can be obtained by adsorptive chromatography. This study is the first scientific insight into the isotherm and kinetic studies of kafirin adsorption on anion-and cation-exchange resins for practical applications in preparative scale chromatography. Adsorption isotherms of kafirin were determined for five anion- and two cation-exchange resins in batch systems. Isotherm parameters such as maximum binding capacity and dissociation constant were determined from Langmuir isotherm, and adsorptive capacity and affinity constant from Freundlich isotherm. Langmuir isotherm was found to fit the adsorption equilibrium data well. Batch uptake kinetics for kafirin adsorption on these resins was also carried out and critical parameters including the diffusion coefficient, film mass transfer coefficient, and Biot number for film-pore diffusion model were calculated. Both the isotherm and the kinetic parameters were considered for selection of appropriate resin for kafirin purification. UNOsphere Q (78.26 mg/ml) and Toyopearl SP-650M (57.4 mg/ml) were found to offer better kafirin binding capacities and interaction strength with excellent uptake kinetics under moderate operating conditions. With these adsorbents, film diffusion resistance was found to be major governing factor for adsorption (Bi < 10 and δ < 1). Based on designer objective function, UNOsphere Q was found be best adsorbent for binding of kafirin. The data presented is valuable for designing large scale preparative adsorptive chromatographic kafirin purification systems. |
| first_indexed | 2025-11-14T07:37:22Z |
| format | Journal Article |
| id | curtin-20.500.11937-21014 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:37:22Z |
| publishDate | 2014 |
| publisher | Elsevier BV |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-210142017-10-02T02:28:04Z Kafirin Adsorption on Ion-Exchange resins: Isotherm and Kinetic Studies Kumar, P. Lau, P. Kale, S. Johnson, Stuart Pareek, Vishnu Utikar, Ranjeet Lali, A. Kafirin Kineticsa Prolamin Adsorption isotherm Chromatography Today kafirin is found to be useful in designing delayed delivery systems and coatings of pharmaceuticals and nutraceuticals where its purity is important and this can be obtained by adsorptive chromatography. This study is the first scientific insight into the isotherm and kinetic studies of kafirin adsorption on anion-and cation-exchange resins for practical applications in preparative scale chromatography. Adsorption isotherms of kafirin were determined for five anion- and two cation-exchange resins in batch systems. Isotherm parameters such as maximum binding capacity and dissociation constant were determined from Langmuir isotherm, and adsorptive capacity and affinity constant from Freundlich isotherm. Langmuir isotherm was found to fit the adsorption equilibrium data well. Batch uptake kinetics for kafirin adsorption on these resins was also carried out and critical parameters including the diffusion coefficient, film mass transfer coefficient, and Biot number for film-pore diffusion model were calculated. Both the isotherm and the kinetic parameters were considered for selection of appropriate resin for kafirin purification. UNOsphere Q (78.26 mg/ml) and Toyopearl SP-650M (57.4 mg/ml) were found to offer better kafirin binding capacities and interaction strength with excellent uptake kinetics under moderate operating conditions. With these adsorbents, film diffusion resistance was found to be major governing factor for adsorption (Bi < 10 and δ < 1). Based on designer objective function, UNOsphere Q was found be best adsorbent for binding of kafirin. The data presented is valuable for designing large scale preparative adsorptive chromatographic kafirin purification systems. 2014 Journal Article http://hdl.handle.net/20.500.11937/21014 10.1016/j.chroma.2014.06.035 Elsevier BV restricted |
| spellingShingle | Kafirin Kineticsa Prolamin Adsorption isotherm Chromatography Kumar, P. Lau, P. Kale, S. Johnson, Stuart Pareek, Vishnu Utikar, Ranjeet Lali, A. Kafirin Adsorption on Ion-Exchange resins: Isotherm and Kinetic Studies |
| title | Kafirin Adsorption on Ion-Exchange resins: Isotherm and Kinetic Studies |
| title_full | Kafirin Adsorption on Ion-Exchange resins: Isotherm and Kinetic Studies |
| title_fullStr | Kafirin Adsorption on Ion-Exchange resins: Isotherm and Kinetic Studies |
| title_full_unstemmed | Kafirin Adsorption on Ion-Exchange resins: Isotherm and Kinetic Studies |
| title_short | Kafirin Adsorption on Ion-Exchange resins: Isotherm and Kinetic Studies |
| title_sort | kafirin adsorption on ion-exchange resins: isotherm and kinetic studies |
| topic | Kafirin Kineticsa Prolamin Adsorption isotherm Chromatography |
| url | http://hdl.handle.net/20.500.11937/21014 |