One step preparation and electrochemical analysis of IQS, a cell–cell communication signal in the nosocomial pathogen Pseudomonas aeruginosa

Pseudomonas aeruginosa uses a hierarchical cell–cell communication system consisting of a number of regulatory elements to coordinate the expression of bacterial virulence genes. Sensitive detection of quorum sensing (QS) molecules has the potential for early identification of P. aeruginosa facilita...

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Main Authors: Shang, F., Muimhneacháin, E., Reen, F., Buzid, A., O'Gara, Fergal, Luong, J., Glennon, J., McGlacken, G.
Format: Journal Article
Published: Pergamon 2014
Online Access:http://hdl.handle.net/20.500.11937/20987
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author Shang, F.
Muimhneacháin, E.
Reen, F.
Buzid, A.
O'Gara, Fergal
Luong, J.
Glennon, J.
McGlacken, G.
author_facet Shang, F.
Muimhneacháin, E.
Reen, F.
Buzid, A.
O'Gara, Fergal
Luong, J.
Glennon, J.
McGlacken, G.
author_sort Shang, F.
building Curtin Institutional Repository
collection Online Access
description Pseudomonas aeruginosa uses a hierarchical cell–cell communication system consisting of a number of regulatory elements to coordinate the expression of bacterial virulence genes. Sensitive detection of quorum sensing (QS) molecules has the potential for early identification of P. aeruginosa facilitating early medical intervention. A recently isolated cell–cell communication molecule, a thiazole termed IQS, can bypass the las QS system of P. aeruginosa under times of stress, activating a subset of QS-controlled genes. This compound offers a new target for pathogen detection and has been prepared in a one step protocol. A simple electrochemical strategy was employed for its sensitive detection using boron-doped diamond and glassy carbon electrodes by cyclic voltammetry and amperometry.
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institution Curtin University Malaysia
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publishDate 2014
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spelling curtin-20.500.11937-209872017-09-13T13:43:51Z One step preparation and electrochemical analysis of IQS, a cell–cell communication signal in the nosocomial pathogen Pseudomonas aeruginosa Shang, F. Muimhneacháin, E. Reen, F. Buzid, A. O'Gara, Fergal Luong, J. Glennon, J. McGlacken, G. Pseudomonas aeruginosa uses a hierarchical cell–cell communication system consisting of a number of regulatory elements to coordinate the expression of bacterial virulence genes. Sensitive detection of quorum sensing (QS) molecules has the potential for early identification of P. aeruginosa facilitating early medical intervention. A recently isolated cell–cell communication molecule, a thiazole termed IQS, can bypass the las QS system of P. aeruginosa under times of stress, activating a subset of QS-controlled genes. This compound offers a new target for pathogen detection and has been prepared in a one step protocol. A simple electrochemical strategy was employed for its sensitive detection using boron-doped diamond and glassy carbon electrodes by cyclic voltammetry and amperometry. 2014 Journal Article http://hdl.handle.net/20.500.11937/20987 10.1016/j.bmcl.2014.08.023 Pergamon restricted
spellingShingle Shang, F.
Muimhneacháin, E.
Reen, F.
Buzid, A.
O'Gara, Fergal
Luong, J.
Glennon, J.
McGlacken, G.
One step preparation and electrochemical analysis of IQS, a cell–cell communication signal in the nosocomial pathogen Pseudomonas aeruginosa
title One step preparation and electrochemical analysis of IQS, a cell–cell communication signal in the nosocomial pathogen Pseudomonas aeruginosa
title_full One step preparation and electrochemical analysis of IQS, a cell–cell communication signal in the nosocomial pathogen Pseudomonas aeruginosa
title_fullStr One step preparation and electrochemical analysis of IQS, a cell–cell communication signal in the nosocomial pathogen Pseudomonas aeruginosa
title_full_unstemmed One step preparation and electrochemical analysis of IQS, a cell–cell communication signal in the nosocomial pathogen Pseudomonas aeruginosa
title_short One step preparation and electrochemical analysis of IQS, a cell–cell communication signal in the nosocomial pathogen Pseudomonas aeruginosa
title_sort one step preparation and electrochemical analysis of iqs, a cell–cell communication signal in the nosocomial pathogen pseudomonas aeruginosa
url http://hdl.handle.net/20.500.11937/20987