Comparison of two agricultural wastes for phenol removal via peroxidase-catalyzed enzymatic approach
© The Authors, published by EDP Sciences, 2016. Agricultural wastes of jicama and luffa skin peels were used as the source for peroxidase extraction. The extracted crude enzymes showed similar activities, 1.34U/mL and 1.22U/mL for jicama and luffa peroxidase respectively. These peroxidases were used...
| Main Authors: | , , , |
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| Format: | Conference Paper |
| Published: |
2016
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| Online Access: | http://hdl.handle.net/20.500.11937/20684 |
| _version_ | 1848750376850817024 |
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| author | Chiong, T. Khor, Ee Danquah, Michaerl Lau, S. |
| author_facet | Chiong, T. Khor, Ee Danquah, Michaerl Lau, S. |
| author_sort | Chiong, T. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © The Authors, published by EDP Sciences, 2016. Agricultural wastes of jicama and luffa skin peels were used as the source for peroxidase extraction. The extracted crude enzymes showed similar activities, 1.34U/mL and 1.22U/mL for jicama and luffa peroxidase respectively. These peroxidases were used to treat phenol under varying operating conditions of buffer pH, hydrogen peroxide concentration, enzyme volume and temperature. Jicama peroxidase demonstrated a phenol removal efficiency of approximately 90% at buffer pH 7, 1mM hydrogen peroxide using 1.5mL enzyme at 25°C. Luffa peroxidase required a higher dosage of hydrogen peroxide, and exhibited a removal efficiency of 84% at 8mM with other operating conditions same as jicama peroxidase. Jicama peroxidase is sensitive to pH change and more susceptible to thermal denaturation. Luffa peroxidase showed a better stability in terms of temperature. |
| first_indexed | 2025-11-14T07:35:51Z |
| format | Conference Paper |
| id | curtin-20.500.11937-20684 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:35:51Z |
| publishDate | 2016 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-206842017-09-13T13:50:22Z Comparison of two agricultural wastes for phenol removal via peroxidase-catalyzed enzymatic approach Chiong, T. Khor, Ee Danquah, Michaerl Lau, S. © The Authors, published by EDP Sciences, 2016. Agricultural wastes of jicama and luffa skin peels were used as the source for peroxidase extraction. The extracted crude enzymes showed similar activities, 1.34U/mL and 1.22U/mL for jicama and luffa peroxidase respectively. These peroxidases were used to treat phenol under varying operating conditions of buffer pH, hydrogen peroxide concentration, enzyme volume and temperature. Jicama peroxidase demonstrated a phenol removal efficiency of approximately 90% at buffer pH 7, 1mM hydrogen peroxide using 1.5mL enzyme at 25°C. Luffa peroxidase required a higher dosage of hydrogen peroxide, and exhibited a removal efficiency of 84% at 8mM with other operating conditions same as jicama peroxidase. Jicama peroxidase is sensitive to pH change and more susceptible to thermal denaturation. Luffa peroxidase showed a better stability in terms of temperature. 2016 Conference Paper http://hdl.handle.net/20.500.11937/20684 10.1051/matecconf/20166901002 fulltext |
| spellingShingle | Chiong, T. Khor, Ee Danquah, Michaerl Lau, S. Comparison of two agricultural wastes for phenol removal via peroxidase-catalyzed enzymatic approach |
| title | Comparison of two agricultural wastes for phenol removal via peroxidase-catalyzed enzymatic approach |
| title_full | Comparison of two agricultural wastes for phenol removal via peroxidase-catalyzed enzymatic approach |
| title_fullStr | Comparison of two agricultural wastes for phenol removal via peroxidase-catalyzed enzymatic approach |
| title_full_unstemmed | Comparison of two agricultural wastes for phenol removal via peroxidase-catalyzed enzymatic approach |
| title_short | Comparison of two agricultural wastes for phenol removal via peroxidase-catalyzed enzymatic approach |
| title_sort | comparison of two agricultural wastes for phenol removal via peroxidase-catalyzed enzymatic approach |
| url | http://hdl.handle.net/20.500.11937/20684 |