Investigating the role of polyols in Cladosporium fulvum during growth under hyperosmotic stress and in planta
The role of the large amounts of polyols accumulated by the fungal tomato pathogen, Cladosporium fulvum (syn. Fulvia fulva, Cooke) both in planta and in axenic cultures has been examined. Arabinitol and glycerol accumulated in response to hyper-osmotic stress in vitro. Mannitol levels were lower in...
| Main Authors: | , , |
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| Format: | Journal Article |
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2003
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| Online Access: | http://hdl.handle.net/20.500.11937/20491 |
| _version_ | 1848750319512584192 |
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| author | Clark, A. Blissett, K. Oliver, Richard |
| author_facet | Clark, A. Blissett, K. Oliver, Richard |
| author_sort | Clark, A. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The role of the large amounts of polyols accumulated by the fungal tomato pathogen, Cladosporium fulvum (syn. Fulvia fulva, Cooke) both in planta and in axenic cultures has been examined. Arabinitol and glycerol accumulated in response to hyper-osmotic stress in vitro. Mannitol levels were lower in osmo-stressed mycelium. 13C NMR spectroscopy indicated that carbon flow from glucose to mannitol was redirected to arabinitol and glycerol in hyper-osmotic conditions. Infected tomato (Lycopersicon esculentum Mill.) plants contained all three polyols whereas glycerol was the only polyol present in uninfected plants, suggesting that the mannitol and arabinitol were of fungal origin. Substantially higher levels of arabinitol and glycerol were present in infected plants that were subjected to a restricted watering regime compared to fully watered plants. The results suggest that a primary role of fungal arabinitol and glycerol, but not mannitol, is osmoregulation and that water acquisition is an important aspect of pathogenicity. |
| first_indexed | 2025-11-14T07:34:57Z |
| format | Journal Article |
| id | curtin-20.500.11937-20491 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:34:57Z |
| publishDate | 2003 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-204912017-09-13T15:59:26Z Investigating the role of polyols in Cladosporium fulvum during growth under hyperosmotic stress and in planta Clark, A. Blissett, K. Oliver, Richard The role of the large amounts of polyols accumulated by the fungal tomato pathogen, Cladosporium fulvum (syn. Fulvia fulva, Cooke) both in planta and in axenic cultures has been examined. Arabinitol and glycerol accumulated in response to hyper-osmotic stress in vitro. Mannitol levels were lower in osmo-stressed mycelium. 13C NMR spectroscopy indicated that carbon flow from glucose to mannitol was redirected to arabinitol and glycerol in hyper-osmotic conditions. Infected tomato (Lycopersicon esculentum Mill.) plants contained all three polyols whereas glycerol was the only polyol present in uninfected plants, suggesting that the mannitol and arabinitol were of fungal origin. Substantially higher levels of arabinitol and glycerol were present in infected plants that were subjected to a restricted watering regime compared to fully watered plants. The results suggest that a primary role of fungal arabinitol and glycerol, but not mannitol, is osmoregulation and that water acquisition is an important aspect of pathogenicity. 2003 Journal Article http://hdl.handle.net/20.500.11937/20491 10.1007/s00425-002-0833-2 restricted |
| spellingShingle | Clark, A. Blissett, K. Oliver, Richard Investigating the role of polyols in Cladosporium fulvum during growth under hyperosmotic stress and in planta |
| title | Investigating the role of polyols in Cladosporium fulvum during growth under hyperosmotic stress and in planta |
| title_full | Investigating the role of polyols in Cladosporium fulvum during growth under hyperosmotic stress and in planta |
| title_fullStr | Investigating the role of polyols in Cladosporium fulvum during growth under hyperosmotic stress and in planta |
| title_full_unstemmed | Investigating the role of polyols in Cladosporium fulvum during growth under hyperosmotic stress and in planta |
| title_short | Investigating the role of polyols in Cladosporium fulvum during growth under hyperosmotic stress and in planta |
| title_sort | investigating the role of polyols in cladosporium fulvum during growth under hyperosmotic stress and in planta |
| url | http://hdl.handle.net/20.500.11937/20491 |