Could natural killer cells compensate for impaired CD4+ T-cell responses to CMV in HIV patients responding to antiretroviral therapy?
We evaluated NK cell subsets and functions in previously immunodeficient HIV patients responding to ART. Cytokine receptor mRNA was quantitated in purified CD56+ cells. Data were correlated with CD4+ T-cell counts and IFN? responses to CMV. NK cell IFN? responses to K562 cells and proportions of CD5...
| Main Authors: | , , , |
|---|---|
| Format: | Journal Article |
| Published: |
Academic Press
2009
|
| Online Access: | http://hdl.handle.net/20.500.11937/20399 |
| _version_ | 1848750294664478720 |
|---|---|
| author | Tan, D. Fernandez, S. French, M. Price, Patricia |
| author_facet | Tan, D. Fernandez, S. French, M. Price, Patricia |
| author_sort | Tan, D. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | We evaluated NK cell subsets and functions in previously immunodeficient HIV patients responding to ART. Cytokine receptor mRNA was quantitated in purified CD56+ cells. Data were correlated with CD4+ T-cell counts and IFN? responses to CMV. NK cell IFN? responses to K562 cells and proportions of CD56lo NK cells were correlated in patients (p < 0.001) and both were lower than in controls (p < 0.001 and p = 0.008, respectively), so all patients had poor NK cell function. Proportions of CD56hi NK cells correlated inversely with current CD4+ T-cell counts (p = 0.006) and perforin expression in CD56hi NK cells was higher in patients than controls (p < 0.05). Hence increased proportions and cytolytic function of CD56hi NK cells may partially compensate for CD4+ T-cell deficiency. NK cell IFN? responses correlated inversely with expression of IL-10 and IL-12 receptor mRNA. Expression of these transcripts is reduced by exposure to the cytokines, which may reflect immune activation in immunodeficient patients. © 2009 Elsevier Inc. All rights reserved. |
| first_indexed | 2025-11-14T07:34:33Z |
| format | Journal Article |
| id | curtin-20.500.11937-20399 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:34:33Z |
| publishDate | 2009 |
| publisher | Academic Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-203992017-09-13T13:49:37Z Could natural killer cells compensate for impaired CD4+ T-cell responses to CMV in HIV patients responding to antiretroviral therapy? Tan, D. Fernandez, S. French, M. Price, Patricia We evaluated NK cell subsets and functions in previously immunodeficient HIV patients responding to ART. Cytokine receptor mRNA was quantitated in purified CD56+ cells. Data were correlated with CD4+ T-cell counts and IFN? responses to CMV. NK cell IFN? responses to K562 cells and proportions of CD56lo NK cells were correlated in patients (p < 0.001) and both were lower than in controls (p < 0.001 and p = 0.008, respectively), so all patients had poor NK cell function. Proportions of CD56hi NK cells correlated inversely with current CD4+ T-cell counts (p = 0.006) and perforin expression in CD56hi NK cells was higher in patients than controls (p < 0.05). Hence increased proportions and cytolytic function of CD56hi NK cells may partially compensate for CD4+ T-cell deficiency. NK cell IFN? responses correlated inversely with expression of IL-10 and IL-12 receptor mRNA. Expression of these transcripts is reduced by exposure to the cytokines, which may reflect immune activation in immunodeficient patients. © 2009 Elsevier Inc. All rights reserved. 2009 Journal Article http://hdl.handle.net/20.500.11937/20399 10.1016/j.clim.2009.03.518 Academic Press restricted |
| spellingShingle | Tan, D. Fernandez, S. French, M. Price, Patricia Could natural killer cells compensate for impaired CD4+ T-cell responses to CMV in HIV patients responding to antiretroviral therapy? |
| title | Could natural killer cells compensate for impaired CD4+ T-cell responses to CMV in HIV patients responding to antiretroviral therapy? |
| title_full | Could natural killer cells compensate for impaired CD4+ T-cell responses to CMV in HIV patients responding to antiretroviral therapy? |
| title_fullStr | Could natural killer cells compensate for impaired CD4+ T-cell responses to CMV in HIV patients responding to antiretroviral therapy? |
| title_full_unstemmed | Could natural killer cells compensate for impaired CD4+ T-cell responses to CMV in HIV patients responding to antiretroviral therapy? |
| title_short | Could natural killer cells compensate for impaired CD4+ T-cell responses to CMV in HIV patients responding to antiretroviral therapy? |
| title_sort | could natural killer cells compensate for impaired cd4+ t-cell responses to cmv in hiv patients responding to antiretroviral therapy? |
| url | http://hdl.handle.net/20.500.11937/20399 |