Tunable two-dimensional array patterning of antibody annuli through microsphere templating
Protein microarrays are of great research interest because of their potential application as biosensors for high-throughput protein and pathogen screening technologies. In this active area, there is a lack of techniques that can result in annulus-shaped protein structures (e.g., for the utilization...
| Main Authors: | , |
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| Format: | Journal Article |
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American Chemical Society
2010
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| Online Access: | http://hdl.handle.net/20.500.11937/20029 |
| _version_ | 1848750195588726784 |
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| author | Wolf, C. Li, Qin |
| author_facet | Wolf, C. Li, Qin |
| author_sort | Wolf, C. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Protein microarrays are of great research interest because of their potential application as biosensors for high-throughput protein and pathogen screening technologies. In this active area, there is a lack of techniques that can result in annulus-shaped protein structures (e.g., for the utilization of curved surfaces for enhanced protein−protein interactions and the detection of antigens). We present a new technique employing colloidal templating to yield large-scale (∼cm2) 2D arrays of antibodies against Escherichia coli K12 and enhanced green fluorescent protein (eGFP) on a versatile glass surface. The antibodies are swept to reside around the templating microspheres during solution drying and physically adsorb onto the glass. After the microspheres are removed, an array of annulus-shaped antibody structures is formed. We demonstrate the preserved antibody structure and functionality by binding the specific antigens and secondary antibodies, respectively, which paves the way for the binding of biomolecules and pathogens such as bacteria and viruses. The structures were investigated via atomic force, confocal, and fluorescence microscopy. Operational factors such as the drying time, temperature, and humidity as well as the presence of surfactants in the antibody solution were tuned to obtain a stable antibody structure. |
| first_indexed | 2025-11-14T07:32:58Z |
| format | Journal Article |
| id | curtin-20.500.11937-20029 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:32:58Z |
| publishDate | 2010 |
| publisher | American Chemical Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-200292017-09-13T13:48:57Z Tunable two-dimensional array patterning of antibody annuli through microsphere templating Wolf, C. Li, Qin Protein microarrays are of great research interest because of their potential application as biosensors for high-throughput protein and pathogen screening technologies. In this active area, there is a lack of techniques that can result in annulus-shaped protein structures (e.g., for the utilization of curved surfaces for enhanced protein−protein interactions and the detection of antigens). We present a new technique employing colloidal templating to yield large-scale (∼cm2) 2D arrays of antibodies against Escherichia coli K12 and enhanced green fluorescent protein (eGFP) on a versatile glass surface. The antibodies are swept to reside around the templating microspheres during solution drying and physically adsorb onto the glass. After the microspheres are removed, an array of annulus-shaped antibody structures is formed. We demonstrate the preserved antibody structure and functionality by binding the specific antigens and secondary antibodies, respectively, which paves the way for the binding of biomolecules and pathogens such as bacteria and viruses. The structures were investigated via atomic force, confocal, and fluorescence microscopy. Operational factors such as the drying time, temperature, and humidity as well as the presence of surfactants in the antibody solution were tuned to obtain a stable antibody structure. 2010 Journal Article http://hdl.handle.net/20.500.11937/20029 10.1021/la101212y American Chemical Society restricted |
| spellingShingle | Wolf, C. Li, Qin Tunable two-dimensional array patterning of antibody annuli through microsphere templating |
| title | Tunable two-dimensional array patterning of antibody annuli through microsphere templating |
| title_full | Tunable two-dimensional array patterning of antibody annuli through microsphere templating |
| title_fullStr | Tunable two-dimensional array patterning of antibody annuli through microsphere templating |
| title_full_unstemmed | Tunable two-dimensional array patterning of antibody annuli through microsphere templating |
| title_short | Tunable two-dimensional array patterning of antibody annuli through microsphere templating |
| title_sort | tunable two-dimensional array patterning of antibody annuli through microsphere templating |
| url | http://hdl.handle.net/20.500.11937/20029 |