Rapid Determination of Carbohydrates in Heroin Drug Seizures Using Capillary Electophoresis with Short-End Injection
A simple and rapid method for the analysis of carbohydrates in heroin samples by capillary electrophoresis utilizing a borate complexation method is described. Separations were performed using an uncoated fused silica capillary, 50 cm × 50 µm I.D. × 360 µm O.D. with an effective separation length of...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Published: |
ASTM International
2005
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| Online Access: | http://hdl.handle.net/20.500.11937/19863 |
| _version_ | 1848750150470598656 |
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| author | Anastos, N. Barnett, N. Lewis, Simon Pearson, J. Kirkbride, K. |
| author_facet | Anastos, N. Barnett, N. Lewis, Simon Pearson, J. Kirkbride, K. |
| author_sort | Anastos, N. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | A simple and rapid method for the analysis of carbohydrates in heroin samples by capillary electrophoresis utilizing a borate complexation method is described. Separations were performed using an uncoated fused silica capillary, 50 cm × 50 µm I.D. × 360 µm O.D. with an effective separation length of 9 cm. The system was run at 60?C with an applied voltage of -8 kilovolts. Injection of each sample was for 1 sec at -50 mbar. UV detection was employed with the wavelength set at 195 nm. The background electrolyte consisted of 65 mM borate, pH 12.0. Samples and standards were prepared in the run buffer containing 2 mg/mL of mannose as an internal standard. Under these conditions a test mixture containing glucose, sucrose, lactose, mannitol and mannose as an internal standard was resolved within 5 min. The method was used to determine the concentration of carbohydrates in heroin seizure samples and synthetic heroin samples. The results were in good agreement with the reported values. |
| first_indexed | 2025-11-14T07:32:15Z |
| format | Journal Article |
| id | curtin-20.500.11937-19863 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:32:15Z |
| publishDate | 2005 |
| publisher | ASTM International |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-198632017-02-28T01:35:24Z Rapid Determination of Carbohydrates in Heroin Drug Seizures Using Capillary Electophoresis with Short-End Injection Anastos, N. Barnett, N. Lewis, Simon Pearson, J. Kirkbride, K. capillary electrophoresis borate carbohydrates short-end injection forensic science heroin seizures A simple and rapid method for the analysis of carbohydrates in heroin samples by capillary electrophoresis utilizing a borate complexation method is described. Separations were performed using an uncoated fused silica capillary, 50 cm × 50 µm I.D. × 360 µm O.D. with an effective separation length of 9 cm. The system was run at 60?C with an applied voltage of -8 kilovolts. Injection of each sample was for 1 sec at -50 mbar. UV detection was employed with the wavelength set at 195 nm. The background electrolyte consisted of 65 mM borate, pH 12.0. Samples and standards were prepared in the run buffer containing 2 mg/mL of mannose as an internal standard. Under these conditions a test mixture containing glucose, sucrose, lactose, mannitol and mannose as an internal standard was resolved within 5 min. The method was used to determine the concentration of carbohydrates in heroin seizure samples and synthetic heroin samples. The results were in good agreement with the reported values. 2005 Journal Article http://hdl.handle.net/20.500.11937/19863 ASTM International restricted |
| spellingShingle | capillary electrophoresis borate carbohydrates short-end injection forensic science heroin seizures Anastos, N. Barnett, N. Lewis, Simon Pearson, J. Kirkbride, K. Rapid Determination of Carbohydrates in Heroin Drug Seizures Using Capillary Electophoresis with Short-End Injection |
| title | Rapid Determination of Carbohydrates in Heroin Drug Seizures Using Capillary Electophoresis with Short-End Injection |
| title_full | Rapid Determination of Carbohydrates in Heroin Drug Seizures Using Capillary Electophoresis with Short-End Injection |
| title_fullStr | Rapid Determination of Carbohydrates in Heroin Drug Seizures Using Capillary Electophoresis with Short-End Injection |
| title_full_unstemmed | Rapid Determination of Carbohydrates in Heroin Drug Seizures Using Capillary Electophoresis with Short-End Injection |
| title_short | Rapid Determination of Carbohydrates in Heroin Drug Seizures Using Capillary Electophoresis with Short-End Injection |
| title_sort | rapid determination of carbohydrates in heroin drug seizures using capillary electophoresis with short-end injection |
| topic | capillary electrophoresis borate carbohydrates short-end injection forensic science heroin seizures |
| url | http://hdl.handle.net/20.500.11937/19863 |