Imaging nuclear, endoplasmic reticulum, and plasma membrane events in real time
Live cell imaging can provide important information on cellular dynamics; however, the full utilisation of this technology has been hampered by the limitations of imaging reagents. Metal-based complexes have the potential to overcome many of the issues common to many current imaging agents. The rhen...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Published: |
Wiley
2016
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| Online Access: | http://purl.org/au-research/grants/arc/FT130100033 http://hdl.handle.net/20.500.11937/19713 |
| _version_ | 1848750108975300608 |
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| author | Bader, C. Sorvina, A. Simpson, Peter Wright, P. Stagni, S. Plush, S. Massi, Massimiliano Brooks, D. |
| author_facet | Bader, C. Sorvina, A. Simpson, Peter Wright, P. Stagni, S. Plush, S. Massi, Massimiliano Brooks, D. |
| author_sort | Bader, C. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Live cell imaging can provide important information on cellular dynamics; however, the full utilisation of this technology has been hampered by the limitations of imaging reagents. Metal-based complexes have the potential to overcome many of the issues common to many current imaging agents. The rhenium (I)-based complex fac-[Re(CO)3(1,10-phenanthroline)(4-pyridyltetrazolate)], herein referred to as ReZolve-ER™, shows promise as a live cell imaging agent with rapid cell uptake, low cytotoxicity, resistance to photobleaching and compatibility with multicolour imaging. ReZolve-ER™ localised to the nuclear membrane/endoplasmic reticulum (ER) and allowed the detection of exocytotic events at the plasma membrane. Thus, we present a new imaging agent for monitoring live cell events in real time, which is ideal for imaging either short- or long-time courses. |
| first_indexed | 2025-11-14T07:31:36Z |
| format | Journal Article |
| id | curtin-20.500.11937-19713 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:31:36Z |
| publishDate | 2016 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-197132022-11-23T02:59:33Z Imaging nuclear, endoplasmic reticulum, and plasma membrane events in real time Bader, C. Sorvina, A. Simpson, Peter Wright, P. Stagni, S. Plush, S. Massi, Massimiliano Brooks, D. Live cell imaging can provide important information on cellular dynamics; however, the full utilisation of this technology has been hampered by the limitations of imaging reagents. Metal-based complexes have the potential to overcome many of the issues common to many current imaging agents. The rhenium (I)-based complex fac-[Re(CO)3(1,10-phenanthroline)(4-pyridyltetrazolate)], herein referred to as ReZolve-ER™, shows promise as a live cell imaging agent with rapid cell uptake, low cytotoxicity, resistance to photobleaching and compatibility with multicolour imaging. ReZolve-ER™ localised to the nuclear membrane/endoplasmic reticulum (ER) and allowed the detection of exocytotic events at the plasma membrane. Thus, we present a new imaging agent for monitoring live cell events in real time, which is ideal for imaging either short- or long-time courses. 2016 Journal Article http://hdl.handle.net/20.500.11937/19713 10.1002/1873-3468.12365 http://purl.org/au-research/grants/arc/FT130100033 Wiley fulltext |
| spellingShingle | Bader, C. Sorvina, A. Simpson, Peter Wright, P. Stagni, S. Plush, S. Massi, Massimiliano Brooks, D. Imaging nuclear, endoplasmic reticulum, and plasma membrane events in real time |
| title | Imaging nuclear, endoplasmic reticulum, and plasma membrane events in real time |
| title_full | Imaging nuclear, endoplasmic reticulum, and plasma membrane events in real time |
| title_fullStr | Imaging nuclear, endoplasmic reticulum, and plasma membrane events in real time |
| title_full_unstemmed | Imaging nuclear, endoplasmic reticulum, and plasma membrane events in real time |
| title_short | Imaging nuclear, endoplasmic reticulum, and plasma membrane events in real time |
| title_sort | imaging nuclear, endoplasmic reticulum, and plasma membrane events in real time |
| url | http://purl.org/au-research/grants/arc/FT130100033 http://hdl.handle.net/20.500.11937/19713 |