The Medicago truncatula ortholog of Arabidopsis EIN2, sickle, is a negative regulator of symbiotic and pathogenic microbial associations
The plant hormone ethylene negatively regulates bacterial infection and nodule formation in legumes in response to symbiotic rhizobia, but the molecular mechanism(s) of ethylene action in symbiosis remain obscure. We have identified and characterized multiple mutant alleles of the MtSkl1 gene, which...
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Published: |
Wiley
2008
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/19692 |
| _version_ | 1848750103346544640 |
|---|---|
| author | Penmetsa, R. Uribe, P. Anderson, J. Lichtenzveig, Judith Gish, John-Charles Nam, Young Woo Engstrom, Eric Xu, Kun Sckisel, Gail Pereira, Mariana Baek, Jong Min Lopez-Meyer, Melina Long, Sharon R. Harrison, Maria J. Singh, Karam B. Kiss, Gyorgy B. Cook, Douglas R. |
| author_facet | Penmetsa, R. Uribe, P. Anderson, J. Lichtenzveig, Judith Gish, John-Charles Nam, Young Woo Engstrom, Eric Xu, Kun Sckisel, Gail Pereira, Mariana Baek, Jong Min Lopez-Meyer, Melina Long, Sharon R. Harrison, Maria J. Singh, Karam B. Kiss, Gyorgy B. Cook, Douglas R. |
| author_sort | Penmetsa, R. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The plant hormone ethylene negatively regulates bacterial infection and nodule formation in legumes in response to symbiotic rhizobia, but the molecular mechanism(s) of ethylene action in symbiosis remain obscure. We have identified and characterized multiple mutant alleles of the MtSkl1 gene, which controls both ethylene sensitivity and nodule numbers. We show that this locus encodes the Medicago truncatula ortholog of the Arabidopsis ethylene signaling protein EIN2. In addition to the well-characterized role of MtSkl1 in rhizobial symbiosis, we show that MtSkl1 is involved in regulating early phases of the symbiotic interaction with mycorrhizal fungi, and in mediating root responses to cytokinin. MtSkl1 also functions in the defense against Rhizoctonia solani and Phytophthora medicaginis, with the latter interaction likely to involve positive feedback amplification of ethylene biosynthesis. Overexpression of the C-terminal domain of MtEIN2 is sufficient to block nodulation responses, consistent with previous reports in Arabidopsis on the activation of ethylene signaling. This same C-terminal region is uniquely conserved throughout the EIN2 homologs of angiosperms, which is consistent with its role as a higher plant-specific innovation essential to EIN2 function. |
| first_indexed | 2025-11-14T07:31:30Z |
| format | Journal Article |
| id | curtin-20.500.11937-19692 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:31:30Z |
| publishDate | 2008 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-196922017-09-13T15:57:25Z The Medicago truncatula ortholog of Arabidopsis EIN2, sickle, is a negative regulator of symbiotic and pathogenic microbial associations Penmetsa, R. Uribe, P. Anderson, J. Lichtenzveig, Judith Gish, John-Charles Nam, Young Woo Engstrom, Eric Xu, Kun Sckisel, Gail Pereira, Mariana Baek, Jong Min Lopez-Meyer, Melina Long, Sharon R. Harrison, Maria J. Singh, Karam B. Kiss, Gyorgy B. Cook, Douglas R. ethylene nodulation Medicago truncatula EIN2 The plant hormone ethylene negatively regulates bacterial infection and nodule formation in legumes in response to symbiotic rhizobia, but the molecular mechanism(s) of ethylene action in symbiosis remain obscure. We have identified and characterized multiple mutant alleles of the MtSkl1 gene, which controls both ethylene sensitivity and nodule numbers. We show that this locus encodes the Medicago truncatula ortholog of the Arabidopsis ethylene signaling protein EIN2. In addition to the well-characterized role of MtSkl1 in rhizobial symbiosis, we show that MtSkl1 is involved in regulating early phases of the symbiotic interaction with mycorrhizal fungi, and in mediating root responses to cytokinin. MtSkl1 also functions in the defense against Rhizoctonia solani and Phytophthora medicaginis, with the latter interaction likely to involve positive feedback amplification of ethylene biosynthesis. Overexpression of the C-terminal domain of MtEIN2 is sufficient to block nodulation responses, consistent with previous reports in Arabidopsis on the activation of ethylene signaling. This same C-terminal region is uniquely conserved throughout the EIN2 homologs of angiosperms, which is consistent with its role as a higher plant-specific innovation essential to EIN2 function. 2008 Journal Article http://hdl.handle.net/20.500.11937/19692 10.1111/j.1365-313X.2008.03531.x Wiley unknown |
| spellingShingle | ethylene nodulation Medicago truncatula EIN2 Penmetsa, R. Uribe, P. Anderson, J. Lichtenzveig, Judith Gish, John-Charles Nam, Young Woo Engstrom, Eric Xu, Kun Sckisel, Gail Pereira, Mariana Baek, Jong Min Lopez-Meyer, Melina Long, Sharon R. Harrison, Maria J. Singh, Karam B. Kiss, Gyorgy B. Cook, Douglas R. The Medicago truncatula ortholog of Arabidopsis EIN2, sickle, is a negative regulator of symbiotic and pathogenic microbial associations |
| title | The Medicago truncatula ortholog of Arabidopsis EIN2, sickle, is a negative regulator of symbiotic and pathogenic microbial associations |
| title_full | The Medicago truncatula ortholog of Arabidopsis EIN2, sickle, is a negative regulator of symbiotic and pathogenic microbial associations |
| title_fullStr | The Medicago truncatula ortholog of Arabidopsis EIN2, sickle, is a negative regulator of symbiotic and pathogenic microbial associations |
| title_full_unstemmed | The Medicago truncatula ortholog of Arabidopsis EIN2, sickle, is a negative regulator of symbiotic and pathogenic microbial associations |
| title_short | The Medicago truncatula ortholog of Arabidopsis EIN2, sickle, is a negative regulator of symbiotic and pathogenic microbial associations |
| title_sort | medicago truncatula ortholog of arabidopsis ein2, sickle, is a negative regulator of symbiotic and pathogenic microbial associations |
| topic | ethylene nodulation Medicago truncatula EIN2 |
| url | http://hdl.handle.net/20.500.11937/19692 |