Antibody-Carbohydrate Recognition from Docked Ensembles Using the AutoMap Procedure.

Carbohydrate-protein recognition is vital to many processes in health and disease. In particular, elucidation of the structural basis of carbohydrate binding is important to the development of oligosaccharides and oligosaccharide mimetics as vaccines for infectious diseases and cancer. Computational...

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Main Authors: Dingjan, T., Agostino, M., Ramsland, Paul, Yuriev, E.
Format: Journal Article
Published: 2015
Online Access:http://hdl.handle.net/20.500.11937/19193
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author Dingjan, T.
Agostino, M.
Ramsland, Paul
Yuriev, E.
author_facet Dingjan, T.
Agostino, M.
Ramsland, Paul
Yuriev, E.
author_sort Dingjan, T.
building Curtin Institutional Repository
collection Online Access
description Carbohydrate-protein recognition is vital to many processes in health and disease. In particular, elucidation of the structural basis of carbohydrate binding is important to the development of oligosaccharides and oligosaccharide mimetics as vaccines for infectious diseases and cancer. Computational structural techniques are valuable for the study of carbohydrate-protein recognition due to the challenges associated with experimental determination of carbohydrate-protein complexes. AutoMap is a computer program that we have developed to study protein-ligand recognition. AutoMap determines the interactions taking place in a set of highly ranked poses obtained from molecular docking and processes these to identify the protein residues most likely to be involved in interactions. In this protocol, we describe the use of AutoMap and illustrate its suitability for studying antibody recognition of the Lewis Y tetrasaccharide, which is a potential cancer vaccine antigen.
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publishDate 2015
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spelling curtin-20.500.11937-191932017-09-13T15:43:29Z Antibody-Carbohydrate Recognition from Docked Ensembles Using the AutoMap Procedure. Dingjan, T. Agostino, M. Ramsland, Paul Yuriev, E. Carbohydrate-protein recognition is vital to many processes in health and disease. In particular, elucidation of the structural basis of carbohydrate binding is important to the development of oligosaccharides and oligosaccharide mimetics as vaccines for infectious diseases and cancer. Computational structural techniques are valuable for the study of carbohydrate-protein recognition due to the challenges associated with experimental determination of carbohydrate-protein complexes. AutoMap is a computer program that we have developed to study protein-ligand recognition. AutoMap determines the interactions taking place in a set of highly ranked poses obtained from molecular docking and processes these to identify the protein residues most likely to be involved in interactions. In this protocol, we describe the use of AutoMap and illustrate its suitability for studying antibody recognition of the Lewis Y tetrasaccharide, which is a potential cancer vaccine antigen. 2015 Journal Article http://hdl.handle.net/20.500.11937/19193 10.1007/978-1-4939-2874-3_4 restricted
spellingShingle Dingjan, T.
Agostino, M.
Ramsland, Paul
Yuriev, E.
Antibody-Carbohydrate Recognition from Docked Ensembles Using the AutoMap Procedure.
title Antibody-Carbohydrate Recognition from Docked Ensembles Using the AutoMap Procedure.
title_full Antibody-Carbohydrate Recognition from Docked Ensembles Using the AutoMap Procedure.
title_fullStr Antibody-Carbohydrate Recognition from Docked Ensembles Using the AutoMap Procedure.
title_full_unstemmed Antibody-Carbohydrate Recognition from Docked Ensembles Using the AutoMap Procedure.
title_short Antibody-Carbohydrate Recognition from Docked Ensembles Using the AutoMap Procedure.
title_sort antibody-carbohydrate recognition from docked ensembles using the automap procedure.
url http://hdl.handle.net/20.500.11937/19193