Determination of natural and synthethic estrogenic compounds in coastal lagoon waters by HPLC-Electrospraymass Spectrometry

A new analytical method was developed for the simultaneous determination of estrogenic compounds of natural (estradiol, estriol, estrone) and synthetic origin, both steroidal (ethinylestradiol, mestranol) and non-steroidal (benzophenone, bisphenol-A, diethylstilbestrol, octylphenol, nonylphenol, non...

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Main Authors: Pojana, G., Bonfa, A., Busetti, Francesco, Collarin, A., Marcomini, A.
Format: Journal Article
Published: Taylor & Francis 2004
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/19137
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author Pojana, G.
Bonfa, A.
Busetti, Francesco
Collarin, A.
Marcomini, A.
author_facet Pojana, G.
Bonfa, A.
Busetti, Francesco
Collarin, A.
Marcomini, A.
author_sort Pojana, G.
building Curtin Institutional Repository
collection Online Access
description A new analytical method was developed for the simultaneous determination of estrogenic compounds of natural (estradiol, estriol, estrone) and synthetic origin, both steroidal (ethinylestradiol, mestranol) and non-steroidal (benzophenone, bisphenol-A, diethylstilbestrol, octylphenol, nonylphenol, nonylphenol monoethoxylate carboxylate), in environmental aqueous samples by high-performance liquid chromatography coupled with ion trap-mass spectrometry via electrospray interface (HPLC-ESI-IT-MS). Quantitative MS detection was performed in the negative mode for all compounds except mestranol and benzophenone, which were detected under positive ion conditions. Very low method detection limits (MDLs), between 0.1 and 2.6 ng/L, were achieved in coastal lagoon water samples, while the developed solid-phase-extraction (SPE) procedure permitted simultaneous recovery of all analytes from spiked water samples with yields >70% (7-11 RSD%), except estriol and benzophenone, which were recovered with 60% (9 RSD%) and 50% (11 RSD%) yields, respectively. The proposed method was applied to the analysis of Venice (Italy) lagoon waters, where average concentrations of selected compounds in the 2.8-33 ng/L concentration range were found.
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spelling curtin-20.500.11937-191372017-09-13T16:01:02Z Determination of natural and synthethic estrogenic compounds in coastal lagoon waters by HPLC-Electrospraymass Spectrometry Pojana, G. Bonfa, A. Busetti, Francesco Collarin, A. Marcomini, A. HPLC-ESI-MS Coastal lagoon waters Endocrine disrupting compounds A new analytical method was developed for the simultaneous determination of estrogenic compounds of natural (estradiol, estriol, estrone) and synthetic origin, both steroidal (ethinylestradiol, mestranol) and non-steroidal (benzophenone, bisphenol-A, diethylstilbestrol, octylphenol, nonylphenol, nonylphenol monoethoxylate carboxylate), in environmental aqueous samples by high-performance liquid chromatography coupled with ion trap-mass spectrometry via electrospray interface (HPLC-ESI-IT-MS). Quantitative MS detection was performed in the negative mode for all compounds except mestranol and benzophenone, which were detected under positive ion conditions. Very low method detection limits (MDLs), between 0.1 and 2.6 ng/L, were achieved in coastal lagoon water samples, while the developed solid-phase-extraction (SPE) procedure permitted simultaneous recovery of all analytes from spiked water samples with yields >70% (7-11 RSD%), except estriol and benzophenone, which were recovered with 60% (9 RSD%) and 50% (11 RSD%) yields, respectively. The proposed method was applied to the analysis of Venice (Italy) lagoon waters, where average concentrations of selected compounds in the 2.8-33 ng/L concentration range were found. 2004 Journal Article http://hdl.handle.net/20.500.11937/19137 10.1080/03067310410001729060 Taylor & Francis restricted
spellingShingle HPLC-ESI-MS
Coastal lagoon waters
Endocrine disrupting compounds
Pojana, G.
Bonfa, A.
Busetti, Francesco
Collarin, A.
Marcomini, A.
Determination of natural and synthethic estrogenic compounds in coastal lagoon waters by HPLC-Electrospraymass Spectrometry
title Determination of natural and synthethic estrogenic compounds in coastal lagoon waters by HPLC-Electrospraymass Spectrometry
title_full Determination of natural and synthethic estrogenic compounds in coastal lagoon waters by HPLC-Electrospraymass Spectrometry
title_fullStr Determination of natural and synthethic estrogenic compounds in coastal lagoon waters by HPLC-Electrospraymass Spectrometry
title_full_unstemmed Determination of natural and synthethic estrogenic compounds in coastal lagoon waters by HPLC-Electrospraymass Spectrometry
title_short Determination of natural and synthethic estrogenic compounds in coastal lagoon waters by HPLC-Electrospraymass Spectrometry
title_sort determination of natural and synthethic estrogenic compounds in coastal lagoon waters by hplc-electrospraymass spectrometry
topic HPLC-ESI-MS
Coastal lagoon waters
Endocrine disrupting compounds
url http://hdl.handle.net/20.500.11937/19137