Identification of microsatellites from an extinct moa species using highthroughput (454) sequence data
Genetic variation in microsatellites is rarely examined in the field of ancientDNA (aDNA) due to the low quantity of nuclear DNA in the fossil record together with the lack of characterized nuclear markers in extinct species. 454 sequencing platforms provide a new high-throughput technology capable...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
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Informa Healthcare
2009
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/18655 |
| _version_ | 1848749807435251712 |
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| author | Allentoft, M. Schuster, S. Holdaway, R. Hale, M. McLay, E. Oskam, C. Gilbert, Thomas Spencer, P. Willerslev, E. Bunce, Michael |
| author_facet | Allentoft, M. Schuster, S. Holdaway, R. Hale, M. McLay, E. Oskam, C. Gilbert, Thomas Spencer, P. Willerslev, E. Bunce, Michael |
| author_sort | Allentoft, M. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Genetic variation in microsatellites is rarely examined in the field of ancientDNA (aDNA) due to the low quantity of nuclear DNA in the fossil record together with the lack of characterized nuclear markers in extinct species. 454 sequencing platforms provide a new high-throughput technology capable of generating up to 1 gigabases per run as short (200–400-bp) read lengths. 454 data were generated from the fossil bone of an extinct New Zealand moa (Aves:Dinornithiformes). We identified numerous short tandem repeat (STR) motifs, and here present the successful isolation and characterization of one polymorphic microsatellite (Moa_MS2). Primers designed to flank this locus amplified all three moa species tested here. The presented method proved to be a fast and efficient way of identifying microsatellite markers in ancient DNAtemplates and, depending on biomolecule preservation, has the potential of enabling high-resolution population genetic studies of extinct taxa. As sequence read lengths of the 454 platforms and its competitors (e.g., the SOLEXA and SOLiD platforms) increase, this approach will become increasingly powerful in identifying microsatellites in extinct (and extant) organisms, and will affordnew opportunities to study past biodiversity and extinction processes. |
| first_indexed | 2025-11-14T07:26:48Z |
| format | Journal Article |
| id | curtin-20.500.11937-18655 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:26:48Z |
| publishDate | 2009 |
| publisher | Informa Healthcare |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-186552017-09-13T13:43:51Z Identification of microsatellites from an extinct moa species using highthroughput (454) sequence data Allentoft, M. Schuster, S. Holdaway, R. Hale, M. McLay, E. Oskam, C. Gilbert, Thomas Spencer, P. Willerslev, E. Bunce, Michael high-throughput 454 sequencing ancient DNA microsatellite development extinct species Genetic variation in microsatellites is rarely examined in the field of ancientDNA (aDNA) due to the low quantity of nuclear DNA in the fossil record together with the lack of characterized nuclear markers in extinct species. 454 sequencing platforms provide a new high-throughput technology capable of generating up to 1 gigabases per run as short (200–400-bp) read lengths. 454 data were generated from the fossil bone of an extinct New Zealand moa (Aves:Dinornithiformes). We identified numerous short tandem repeat (STR) motifs, and here present the successful isolation and characterization of one polymorphic microsatellite (Moa_MS2). Primers designed to flank this locus amplified all three moa species tested here. The presented method proved to be a fast and efficient way of identifying microsatellite markers in ancient DNAtemplates and, depending on biomolecule preservation, has the potential of enabling high-resolution population genetic studies of extinct taxa. As sequence read lengths of the 454 platforms and its competitors (e.g., the SOLEXA and SOLiD platforms) increase, this approach will become increasingly powerful in identifying microsatellites in extinct (and extant) organisms, and will affordnew opportunities to study past biodiversity and extinction processes. 2009 Journal Article http://hdl.handle.net/20.500.11937/18655 10.2144/000113086 Informa Healthcare unknown |
| spellingShingle | high-throughput 454 sequencing ancient DNA microsatellite development extinct species Allentoft, M. Schuster, S. Holdaway, R. Hale, M. McLay, E. Oskam, C. Gilbert, Thomas Spencer, P. Willerslev, E. Bunce, Michael Identification of microsatellites from an extinct moa species using highthroughput (454) sequence data |
| title | Identification of microsatellites from an extinct moa species using highthroughput (454) sequence data |
| title_full | Identification of microsatellites from an extinct moa species using highthroughput (454) sequence data |
| title_fullStr | Identification of microsatellites from an extinct moa species using highthroughput (454) sequence data |
| title_full_unstemmed | Identification of microsatellites from an extinct moa species using highthroughput (454) sequence data |
| title_short | Identification of microsatellites from an extinct moa species using highthroughput (454) sequence data |
| title_sort | identification of microsatellites from an extinct moa species using highthroughput (454) sequence data |
| topic | high-throughput 454 sequencing ancient DNA microsatellite development extinct species |
| url | http://hdl.handle.net/20.500.11937/18655 |