An innovative monolithic column preparation for the isolation of 25 kilo base pairs DNA

QR Code

An innovative monolithic column preparation for the isolation of 25 kilo base pairs DNA

The use of large DNAs in preparing multivalent vaccines that will eventually give protective immunity against multiple pathogenic microbes is becoming a major debate nowadays. One of the important issues in ensuring the successful implementation of the new vaccine technology is the development of a...

Full description

Bibliographic Details
Main Authors:	Ongkudon, C., Pan, S., Danquah, Michael
Format:	Journal Article
Published:	Elsevier BV 2013
Online Access:	http://hdl.handle.net/20.500.11937/18093

Similar Items

Process optimisation for anion exchange monolithic chromatography of 4.2kbp plasmid vaccine (pcDNA3F)
by: Ongkudon, C., et al.
Published: (2010)

Anion exchange chromatography of 4.2 kbp plasmid based vaccine (pcDNA3F) from alkaline lysed E. coli lysate using amino functionalised polymethacrylate conical monolith
by: Ongkudon, C., et al.
Published: (2011)

Versatility of polymethacrylate monoliths for chromatographic purification of biomolecules
by: Roberts, M., et al.
Published: (2009)

Development and characteristics of polymer monoliths for advanced LC bioscreening applications: A review
by: Acquah, C., et al.
Published: (2016)

Nano-Doped Monolithic Materials for Molecular Separation
by: Acquah, C., et al.
Published: (2017)

In-process thermochemical analysis of in situ poly (ethylene glycol methacrylate-co-glycidyl methacrylate) monolithic adsorbent synthesis
by: Acquah, C., et al.
Published: (2016)

Parametric investigation of polymethacrylate monolith synthesis and stability via thermogravimetric characterisation
by: Acquah, C., et al.
Published: (2017)

Thermogravimetric characterization of ex situ polymethacrylate (EDMA-co-GMA) monoliths
by: Acquah, C., et al.
Published: (2017)

Endotoxin removal and plasmid DNA recovery in various metal ion solutions
by: Ongkudon, C., et al.
Published: (2011)

Preparation of macroporous methacrylate monolithic material with convective flow properties for bioseparation: Investigating the kinetics of pore formation and hydrodynamic performance
by: Danquah, Michael, et al.
Published: (2008)

Rekabentuk Sistem Pembacaan Meter Kilo Watt Jam ( Kwj ) Secara Telemetri
by: Alifuddin, Suhaili
Published: (2006)

Analysis of selective metal-salt-induced endotoxin precipitation in plasmid DNA purification using improved limulus amoebocyte lysate assay and central composite design
by: Ongkudon, C., et al.
Published: (2011)

Comparative study of monolithic and precast concrete beam-to-column connections
by: Abd. Rahman, Ahmad Baharuddin, et al.
Published: (2008)

A thermal expulsion approach to homogeneous large-volume methacrylate monolith preparation; enabling large-scale rapid purification of biomolecules
by: Danquah, Michael, et al.
Published: (2008)

Removal of endotoxins from plasmid DNA: Analysis of aggregative interaction of mobile divalent metal cations with endotoxins and plasmid DNA
by: Ongkudon, C., et al.
Published: (2012)

Poly(Styrene-Divinyl Benzene)-Based Monolithic Column For High Performance Liquid Chromatography
by: Abd. Aziz, Norsyariza, et al.
Published: (2006)

Chromatographic characterisation of aptamer-modified poly(EDMA-co-GMA) monolithic disk format for protein binding and separation
by: Acquah, C., et al.
Published: (2018)

Large-volume methacrylate monolith for plasmid purification. Process engineering approach to synthesis and application
by: Danquah, Michael, et al.
Published: (2008)

Rapid production of a plasmid DNA encoding a malaria vaccine candidate via amino-functionalized poly(GMA-co-EDMA) monolith
by: Danquah, Michael, et al.
Published: (2008)

Dynamic Analysis and Design of Monolithic and Segmental Concrete Bridge Columns against Impact Loads
by: Do, Van Tin
Published: (2019)

Determination of selected neurotransmitter metabolites using monolithic column chromatography coupled with chemiluminescence detection
by: Adcock, J., et al.
Published: (2005)

β-carotene adsorption onto mesoporous carbon-coated monolith column: dynamic studies
by: Muhammad,, et al.
Published: (2013)

Cultivation of E. coli carrying a plasmid-based Measles vaccine construct (4.2 kbp pcDNA3F) employing medium optimisation and pH-temperature induction techniques
by: Ongkudon, C., et al.
Published: (2011)

Preparation and characterization of furfuryl alcohol derived carbon coated monolith
by: Darmadi,, et al.
Published: (2008)

Preparation of a Monolith with Covalently Bound Bovine Serum Albumin for Capillary Electrochromatography
by: Yao, C., et al.
Published: (2012)

Bioprocess challenges to the isolation and purification of bioactive peptides
by: Agyei, D., et al.
Published: (2016)

Hydrogen Bond Disruption in DNA Base Pairs from 14C Transmutation
by: Sassi, Michel, et al.
Published: (2014)

Supramolecular self-assembly of DNA and RNA base pair functionalised porphyrins
by: Toft, Charles
Published: (2019)

Rekabentuk Sistem Pembacaan Meter Kilo Watt Jam (KWJ) Secara Telemetri [TK399. S947 2007 f rb].
by: Alifuddin, Suhaili
Published: (2006)

A parametric study of a monolithic microfluidic system for on-chip biomolecular separation
by: Chan, A., et al.
Published: (2014)

Preparation of Furfuryl Alcohol-Derived Activated Carbon Monolith for Liquid Adsorption
by: Darmadi,
Published: (2009)

Analysis of Endotoxins Removal from Clarified Cell Lysates by Divalent Metal Cations-Induced Aggregation
by: Ongkudon, C., et al.
Published: (2014)

Mitigating the looming vaccine crisis: production and delivery of plasmid-based vaccines
by: Ongkudon, C., et al.
Published: (2011)

Theoretical studies of bioferroelectricity in DNA/RNA nucleobases and their base pairs / Yam See Chuan
by: Yam, See Chuan
Published: (2019)

Evaluation of porogen factors for the preparation of ion imprinted polymer monoliths used in mercury removal
by: Ab. Rahman, Siti Khadijah, et al.
Published: (2018)

Preparation, characterization and methylene blue adsorption of surfactant-templated mesoporous carbon coated monolith
by: Malekbala, Mohamad Rasool
Published: (2012)

Green Approaches Involving Liquid Phase Microextraction, Monolithic Column And Capacitively Coupled Contactless Conductivity Detection In Flow Techniques
by: Makahleh, Ahmad (Moh’d Ali) Hasan
Published: (2011)

Rapid determination of Papaver somniferum alkaloids in process streams using monolithic column high-performance liquid chromatography with chemiluminescence detection
by: Costin, J., et al.
Published: (2007)

Breaking the DNA-vaccine bottleneck
by: Danquah, Michael, et al.
Published: (2011)

Use of Box-Behnken design in the preparation of mesoporous carbon coated monolith - BET surface area
by: Darmadi,, et al.
Published: (2011)