An innovative monolithic column preparation for the isolation of 25 kilo base pairs DNA
The use of large DNAs in preparing multivalent vaccines that will eventually give protective immunity against multiple pathogenic microbes is becoming a major debate nowadays. One of the important issues in ensuring the successful implementation of the new vaccine technology is the development of a...
| Main Authors: | , , |
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| Format: | Journal Article |
| Published: |
Elsevier BV
2013
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| Online Access: | http://hdl.handle.net/20.500.11937/18093 |
| _version_ | 1848749645339033600 |
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| author | Ongkudon, C. Pan, S. Danquah, Michael |
| author_facet | Ongkudon, C. Pan, S. Danquah, Michael |
| author_sort | Ongkudon, C. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The use of large DNAs in preparing multivalent vaccines that will eventually give protective immunity against multiple pathogenic microbes is becoming a major debate nowadays. One of the important issues in ensuring the successful implementation of the new vaccine technology is the development of a chromatographic technique that can handle larger DNAs. This paper reports the development of a novel conical monolithic column format with pore and surface characteristics engineered for the isolation of 25. kbp DNA in a single step fashion. An effective method of eliminating wall channelling, a defect of most conventional monolithic chromatography systems which has caused significant loss of product, was applied to maximise DNA recovery. This method was based on a systematic reduction of wall channel size based on a predetermined correlation between column's back pressure and wall channel size of a particular monolith pore size. |
| first_indexed | 2025-11-14T07:24:14Z |
| format | Journal Article |
| id | curtin-20.500.11937-18093 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:24:14Z |
| publishDate | 2013 |
| publisher | Elsevier BV |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-180932017-09-13T13:43:52Z An innovative monolithic column preparation for the isolation of 25 kilo base pairs DNA Ongkudon, C. Pan, S. Danquah, Michael The use of large DNAs in preparing multivalent vaccines that will eventually give protective immunity against multiple pathogenic microbes is becoming a major debate nowadays. One of the important issues in ensuring the successful implementation of the new vaccine technology is the development of a chromatographic technique that can handle larger DNAs. This paper reports the development of a novel conical monolithic column format with pore and surface characteristics engineered for the isolation of 25. kbp DNA in a single step fashion. An effective method of eliminating wall channelling, a defect of most conventional monolithic chromatography systems which has caused significant loss of product, was applied to maximise DNA recovery. This method was based on a systematic reduction of wall channel size based on a predetermined correlation between column's back pressure and wall channel size of a particular monolith pore size. 2013 Journal Article http://hdl.handle.net/20.500.11937/18093 10.1016/j.chroma.2013.10.011 Elsevier BV restricted |
| spellingShingle | Ongkudon, C. Pan, S. Danquah, Michael An innovative monolithic column preparation for the isolation of 25 kilo base pairs DNA |
| title | An innovative monolithic column preparation for the isolation of 25 kilo base pairs DNA |
| title_full | An innovative monolithic column preparation for the isolation of 25 kilo base pairs DNA |
| title_fullStr | An innovative monolithic column preparation for the isolation of 25 kilo base pairs DNA |
| title_full_unstemmed | An innovative monolithic column preparation for the isolation of 25 kilo base pairs DNA |
| title_short | An innovative monolithic column preparation for the isolation of 25 kilo base pairs DNA |
| title_sort | innovative monolithic column preparation for the isolation of 25 kilo base pairs dna |
| url | http://hdl.handle.net/20.500.11937/18093 |