The incorporation and metabolism of amyloid-B into chylomicron-like lipid emulsions
The aggregation and deposition of amyloid-g (Ag) in the brain is thought to be an early event in the pathology of Alzheimer's disease (AD). Many studies have reported the association of Agwith lipoproteins from plasma suggesting an involvement of lipoprotein particles in Ag transport. Chylomic...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Published: |
IOS Press
2003
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| Online Access: | http://hdl.handle.net/20.500.11937/17668 |
| _version_ | 1848749524839825408 |
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| author | James, Anthony Pal, Sebely Gennat, H. Vine, Donna Mamo, John |
| author_facet | James, Anthony Pal, Sebely Gennat, H. Vine, Donna Mamo, John |
| author_sort | James, Anthony |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The aggregation and deposition of amyloid-g (Ag) in the brain is thought to be an early event in the pathology of Alzheimer's disease (AD). Many studies have reported the association of Agwith lipoproteins from plasma suggesting an involvement of lipoprotein particles in Ag transport. Chylomicron-like lipid emulsions, resembling chylomicrons in composition, size and metabolism were prepared in the presence of [^{125}I]Ag1-40. Ag was found to associate significantly with these lipid emulsions during their preparation. The chylomicron-like emulsions containing Ag were then injected into a lateral ear vein of conscious rabbits and blood sampled at regular intervals up to 30 mins. It was observed that there was no difference in the plasma clearance of [^{125}I]Ag and that of the ^3H-cholesteryl ester, a marker of the emulsion particles, demonstrating that Ag remains associated with these particles throughout both their lipolysis and tissue uptake. Our results show thatg can be metabolised in association with triglyceride rich lipoproteins (TRLs). In addition we report the presence of specific markers of TRLs of hepatic and intestinal origin in human CSF thus suggesting a potential means of cerebral Ag delivery. |
| first_indexed | 2025-11-14T07:22:19Z |
| format | Journal Article |
| id | curtin-20.500.11937-17668 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:22:19Z |
| publishDate | 2003 |
| publisher | IOS Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-176682017-05-30T08:14:26Z The incorporation and metabolism of amyloid-B into chylomicron-like lipid emulsions James, Anthony Pal, Sebely Gennat, H. Vine, Donna Mamo, John The aggregation and deposition of amyloid-g (Ag) in the brain is thought to be an early event in the pathology of Alzheimer's disease (AD). Many studies have reported the association of Agwith lipoproteins from plasma suggesting an involvement of lipoprotein particles in Ag transport. Chylomicron-like lipid emulsions, resembling chylomicrons in composition, size and metabolism were prepared in the presence of [^{125}I]Ag1-40. Ag was found to associate significantly with these lipid emulsions during their preparation. The chylomicron-like emulsions containing Ag were then injected into a lateral ear vein of conscious rabbits and blood sampled at regular intervals up to 30 mins. It was observed that there was no difference in the plasma clearance of [^{125}I]Ag and that of the ^3H-cholesteryl ester, a marker of the emulsion particles, demonstrating that Ag remains associated with these particles throughout both their lipolysis and tissue uptake. Our results show thatg can be metabolised in association with triglyceride rich lipoproteins (TRLs). In addition we report the presence of specific markers of TRLs of hepatic and intestinal origin in human CSF thus suggesting a potential means of cerebral Ag delivery. 2003 Journal Article http://hdl.handle.net/20.500.11937/17668 IOS Press restricted |
| spellingShingle | James, Anthony Pal, Sebely Gennat, H. Vine, Donna Mamo, John The incorporation and metabolism of amyloid-B into chylomicron-like lipid emulsions |
| title | The incorporation and metabolism of amyloid-B into chylomicron-like lipid emulsions |
| title_full | The incorporation and metabolism of amyloid-B into chylomicron-like lipid emulsions |
| title_fullStr | The incorporation and metabolism of amyloid-B into chylomicron-like lipid emulsions |
| title_full_unstemmed | The incorporation and metabolism of amyloid-B into chylomicron-like lipid emulsions |
| title_short | The incorporation and metabolism of amyloid-B into chylomicron-like lipid emulsions |
| title_sort | incorporation and metabolism of amyloid-b into chylomicron-like lipid emulsions |
| url | http://hdl.handle.net/20.500.11937/17668 |