AFLPs are incompatible with RAPD and morphological data in Pennisetum purpureum (Napier grass)
Accessions of Pennisetum purpureum in agricultural research centres in sub-Saharan Africa were genotyped using Amplified Fragment Length Polymorphisms (AFLPs) using primer combinations MluI/MseI. Cluster analyses of resulting bands suggested that the samples could be grouped into five large and five...
| Main Authors: | , , , , |
|---|---|
| Format: | Journal Article |
| Published: |
2009
|
| Online Access: | http://hdl.handle.net/20.500.11937/17475 |
| _version_ | 1848749476122984448 |
|---|---|
| author | Mucina, Ladislav Struwig, M. Mienie, C. van den Berg, J. Buys, M. |
| author_facet | Mucina, Ladislav Struwig, M. Mienie, C. van den Berg, J. Buys, M. |
| author_sort | Mucina, Ladislav |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Accessions of Pennisetum purpureum in agricultural research centres in sub-Saharan Africa were genotyped using Amplified Fragment Length Polymorphisms (AFLPs) using primer combinations MluI/MseI. Cluster analyses of resulting bands suggested that the samples could be grouped into five large and five small-sized clusters. Principal coordinate analysis revealed two major clusters being well separated, with bulk of the samples forming one, poorly structured cluster. Our analyses failed to reveal the regional groupings found in previous studies using a subset of our sample universe and based on RAPDs as well as morphological and agronomical data. The inability of the AFLP data to clearly demarcate accessions is ascribed to either the presence of high levels of genetic uniformity, or nonoptimal primers and/or bad germplasm management practices. The re-registration of all accessions, based on DNA barcoding, is suggested as a means to resolve the lingering problems regarding the identity of accessions. |
| first_indexed | 2025-11-14T07:21:32Z |
| format | Journal Article |
| id | curtin-20.500.11937-17475 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T07:21:32Z |
| publishDate | 2009 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-174752019-05-16T00:47:35Z AFLPs are incompatible with RAPD and morphological data in Pennisetum purpureum (Napier grass) Mucina, Ladislav Struwig, M. Mienie, C. van den Berg, J. Buys, M. Accessions of Pennisetum purpureum in agricultural research centres in sub-Saharan Africa were genotyped using Amplified Fragment Length Polymorphisms (AFLPs) using primer combinations MluI/MseI. Cluster analyses of resulting bands suggested that the samples could be grouped into five large and five small-sized clusters. Principal coordinate analysis revealed two major clusters being well separated, with bulk of the samples forming one, poorly structured cluster. Our analyses failed to reveal the regional groupings found in previous studies using a subset of our sample universe and based on RAPDs as well as morphological and agronomical data. The inability of the AFLP data to clearly demarcate accessions is ascribed to either the presence of high levels of genetic uniformity, or nonoptimal primers and/or bad germplasm management practices. The re-registration of all accessions, based on DNA barcoding, is suggested as a means to resolve the lingering problems regarding the identity of accessions. 2009 Journal Article http://hdl.handle.net/20.500.11937/17475 10.1016/j.bse.2009.09.010 restricted |
| spellingShingle | Mucina, Ladislav Struwig, M. Mienie, C. van den Berg, J. Buys, M. AFLPs are incompatible with RAPD and morphological data in Pennisetum purpureum (Napier grass) |
| title | AFLPs are incompatible with RAPD and morphological data in Pennisetum purpureum (Napier grass) |
| title_full | AFLPs are incompatible with RAPD and morphological data in Pennisetum purpureum (Napier grass) |
| title_fullStr | AFLPs are incompatible with RAPD and morphological data in Pennisetum purpureum (Napier grass) |
| title_full_unstemmed | AFLPs are incompatible with RAPD and morphological data in Pennisetum purpureum (Napier grass) |
| title_short | AFLPs are incompatible with RAPD and morphological data in Pennisetum purpureum (Napier grass) |
| title_sort | aflps are incompatible with rapd and morphological data in pennisetum purpureum (napier grass) |
| url | http://hdl.handle.net/20.500.11937/17475 |